导电高分子纳米粒子负载探针的凝血酶电化学发光适配体传感器

以特异性识别凝血酶的适配体为分子识别物质,以氨基钌联吡啶衍生物（Ru1）为电化学发光信号物质,基于吡咯/N-（2-羧乙基）吡咯纳米粒子（Ppy-pa NPs）负载适配体和Ru1研制了一种高灵敏检测凝血酶的电化学发光适配体传感器.以N-（2-羧乙基）吡咯和吡咯为单体,采用微乳液聚合方法制备了Ppy-pa NPs.通过EDC/NHS将Ru1与Ppy-pa NPs表面的羧基共价连接制备了Ru1功能化Ppy-pa NPs（Ru1-Ppy-pa NPs）.利用核磁共振图谱、傅里叶变换红外光谱和投射电子显微镜图对Ppy-pa NPs和Ru1-Ppy-pa NPs进行了表征.将Ppy-pa NPs Nafion混合物滴涂在石墨电极表面制备成电化学发光化学传感器,可高灵敏检测三丙胺（检出限为3.0×10-8M）.通过电化学氧化将对氨基苯磺酸共价键合在石墨电极表面,将5′修饰氨基凝血酶适配体I（TBA-I）共价连接在对氨基苯磺酸修饰的石墨电极表面制备成电化学发光适配体传感器.将5′修饰氨基凝血酶适配体Ⅱ（TBA-Ⅱ）标记在Ru1-Ppy-pa NPs表面制得电化学发光适配体信号探针（Ppy-pa NPs-Ru1-TBA-II）.当凝血酶存在时,凝血酶与电极表面的TBA-I特异性结合,再与信号探针结合形成夹心结构,产生很强的电化学发光信号.该传感器具有极低的检出限（3.0×10-16M）和良好的选择性.本工作表明以Ppy-pa NPs为电化学光探针的载体可构建高灵敏度和选择性的电化学发光生物传感器.     

A Unique Heteronuclear Metal Organic Framework Based on Amino-acid and N-donor Ancillary Ligand: Synthesis,Structure and Characterization

A novel heteronuclear metal organic framework(MOF) incorporating both transition and alkali metal ions, namely, {(Ni_2K_2L_2·bipy]×6 H_2O]×2 H_2O}n(1, L = N-phosphonomethyl amino diacetic acid, bipy = 4,4?-dipyridine) has been obtained. Its structure was established by single-crystal X-ray diffraction analysis and further characterized by elemental analysis and IR spectroscopy. The complex crystallizes in orthorhombic system, space group Pccn with a = 14.650(11), b = 36.56(3), c = 7.112(5) ? and V = 3809.3(8) ?~3. In this framework, bipy connects adjacent Ni–K heteronuclear coordination units into 1 D railway type chains, which are further propagated into 2 D deck-like array bearing large tunnels through the carboxylic oxygen from N-phosphonomethyl amino diacetic acid. The molar electrical conductivity of heteronuclear complex increases gradually with increasing the temperature. Complex 1 also exhibits strong photoluminescent emission in the visible region.