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邻苯二甲醛衍生高效液相色谱法快速测定血小板中组胺含量 总被引:1,自引:0,他引:1
An HPLC method for the determination of histamine(HA)in platelet.based on pre-column derivatiza-tion with o-phthalaldehvde(OPA ).is described.The HA was allowed to react with OPA at -10℃ for30min.HPLC was performed on a reversed-phase column with isocratic elution using sodium acetate buffer inmethanol as eluent system.A fluorescence detection system was used;excitation was set at 350.0nm andemission was read at 445.5nm,A good linear relationship was found in the range of 3.75×10 ̄(-3)~10. 5nmol. 相似文献
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