STUDY OF LUNG FLUKES FROM PHILIPPINES——A PRELIMINARY REPORT

In this paper taxonomic study on paragonimus including their metacercariae obtainedfrom the crabs collected from Sorsogon, Luzon, Phi1ippines and adult worms and ova obtain-ed through animal feeding, with light microscopy and scanning electron microscopy revealedthat there may exist two or more species of lung flukes in Philippines.The occurrence ofSemisulcospira libertina in addition to Antemelania dactylus (Lea) as an additional snailhost is mentioned for the first time. The chromosome Karyotyping of the adult worms revealed that they belong to thediploid pattern. Electronic scanning showed that the cuticular spines of the Paragonimus from Sorsogonlook like "pineapple ears" both in distribution and In appearance, a new finding that has not been recorded before. In the lungs of a white rat killed 290 days after infection, two adult worms wereobtained within the solitary eyst. These 2 adult worms measured 4.5×2.5mm and 4.5×3.0 mm respectively. Their unusually small size and somewhat round shape suggest that theymay be of a different species from those hitherto reported in the Philippines. Clearcut evidence is presented for the first time, demonstrating the occurrence of the phemomenon of cross fertilization (allogamy) of lung flukes.     

卫氏并殖吸虫病免疫学诊断的研究——卫氏并殖吸虫成虫粗抗原和各部分提纯抗原的理化性质和免疫活性的初步研究

本文已部分提纯一种并殖吸虫抗原(Fr.1)。以Fr.1致敏平板进行间接酶标试验时,不论对并殖吸虫病患者或感染犬的血清,其阳性率均达100％,而用粗抗原致敏平板时,其阳性率各为71.4％和75％,并且Fr.1的特异性也较优于粗抗原。因此,本实验室已建立了一个更有效地诊断并殖吸虫病的间接酶标测定法。     

A PRELIMINARY STUDY ON THE IMMUNODIAGNOSIS OF PARAGONIMIASIS——THE PHYSICO-CHEMICAL AND IMMUNOLOGICAL CHARACTERISTICS OF THE CRUDE AND PARTIALLY PURIFIED ANTIGENS OF THE PARAGONIMUS WESTERMANI (LIAONING)

We have now succeeded in preparing a partially purified antigen (Fr. 1). The positive rates of the indirect ELISA were 100% both for sera of proved human cases and dogs infected with Paragonimus westermani when Fr. 1 was used, whereas the positive rates of the test were 71.4% and 75% on sera from patients and infected dogs respectively when crude antigen was used. Moreover, it is also better than the crude antigen in specificity, so a more reliable test in immunodiagnosis of paragonimiasis is available in our laboratory.     

一种对人能致病的肺吸虫新种——会同肺吸虫的研究

本文报道了在湖南省会同县发现的一种肺吸虫新种,命名为会同肺吸虫Paragonimus hueit''ungensis sp. nov.. 对其成虫的形态特点以及第一、第二中间宿主、雷蚴、尾蚴、囊蚴和动物感染实验的结果进行了描述,并与已知的各种有关肺吸虫进行了对比。从两例当地患儿的皮下结节中获得虫体,均为童虫,其形态与从实验感染动物所获得的虫体基本一致,但生殖器官发育差,在人体内不产虫卵,到处游走,说明会同肺吸虫对人虽有致病性,但人并非其适宜的终末宿主。因此,其临床表现不同于一般肺吸虫病,但易与四川肺吸虫病相混淆,应引起注意。这种肺吸虫的调查研究对当地肺吸虫病的防治工作具有实际意义。     

菲律宾并殖吸虫的初步研究

本文是根据由菲律宾吕宋岛上索尔索贡(Sorsogon)采集的淡水蟹体内分离到的并殖吸虫囊蚴,和用它喂动物后获得的并殖吸虫成虫和虫卵所进行的分类学研究,经光学显微镜和扫描电子显微镜观察,显示在菲律宾可能存在着两种或更多种的并殖吸虫,本文并首次指出放逸短沟蜷可能是除Antemelania dactylus Lea螺以外的另一种第一中间宿主。 成虫的染色体核型研究证明它属于二倍体型,扫描电子显微镜检查发现,并殖吸虫皮棘的外形和分布与菠萝的刺相似,迄今均未见有类似的报道。 在一只感染后290天处死的大白鼠单个肺囊内获得了两条成虫,其大小分别为4.5×2.5毫米和4.5×3.0毫米。由于它们个体特别小,而且略呈圆形,故它们可能不同于菲律宾已报告过的并殖吸虫。同时,我们还首次观察到并殖吸虫间的交叉受精现象。