E玫瑰花形成与猪淋巴细胞内cAMP水平的变化——影响因素

为进一步研究E玫瑰花形成后淋巴细胞内环-磷酸腺苷(cAMP)的变化规律,观察了淋巴细胞与绵羊红血球(SRBC)膜碎片和完整SRBC作用后cAMP的上升情况,结果发现前者的cAMP水平上升幅度远大于后者,而且cAMP的上升幅度与所用的SRBC细胞膜的浓度以及玫瑰花百分率呈正相关的趋势。还发现,玫瑰花形成后,淋巴细胞内的cAMP水平于1分钟内即上升,10分钟达到高峰,8小时左右降至对照水平。     

PAA修饰稀土掺杂NaYF_4上转换发光中空微管的合成及表征

采用水溶性聚合物聚丙烯酸(PAA)调介的水热法,一步合成具有中空微管结构的PAA修饰β-Na YF_4:Yb~(3+)/Er~(3+)上转换材料。通过扫描电镜、X射线衍射等对产物进行了表征。结果表明,合成中空微管晶体具有均匀的尺寸(平均尺寸为2.0μm×0.6μm);产物呈现出纯β-Na YF_4单晶结构,结晶度高;且稀土离子(Yb~(3+)和Er~(3+))被均匀地掺杂在β-Na YF_4单晶的晶格中。红外光谱表明,中空微管结构的β-Na YF_4:Yb~(3+)/Er~(3+)材料表面含有PAA,PAA修饰赋予中空微管结构的β-Na YF_4:Yb~(3+)/Er~(3+)材料在水中具有良好的分散性。荧光光谱表明,该材料在980nm激光激发下具有优异的荧光性能。这种独特的结构,良好的水分散性,较高的荧光强度且表面含有大量的羧基官能团等特性均使得该材料在生物成像、防伪、药物运输等领域具有潜在的应用价值。     

E-ROSETTE FORMATION WITH SRBC AND CHANGES IN cAMP LEVEL OF HOG LYMPHOCYTES——A STUDY OF CERTAIN AFFECTING FACTORS

A further study of cAMP change in porcine lymphocytes after E-rosette formation ispresented in the present report using peripheral blood lymphocytes of pig mixed with differentconcentrations of SRBC membrane as well as SRBC treated with different enzymes formodification of the rosette formation. It has been found that cAMP enhancement in lymphocytefollows the increase in the concentration of SRBC membrane used, and that a similar relationappeared between modification of cAMP and enzyme-treated SRBC in the rosette formation. Ithas also been found that after rosetting wtth SRBC, cAMP level of lymphocytes began to risewithin 1 min, reached its peak at 10 min and then returned to control leveI in about 8 hr.