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A granulocytic Leukemic cell line, called L_(833), has been established through culturing in vitro frommouse bone marrow of transplantable granulocytic leukemia. More than 280 passages have been performedin 3 years. Cell growth has been rapid and stable. Incidence of tumour formation was 100% with variousroutes of inoculation. The nature of granulocytic leukemia was confirmed by examination of cytology,cytochemistry, pathology and ultrastructural changes. Analysis proved chromosomes to be hypodiploid with model number of 39, loss of chromosomes, andpresence of a marker. Besides the chromosomal change as mentioned above, both L_(883-A) and L_(833-B)derived from colonies formed from the L_(833) cells cultuerd in semi-solid agar medium, have their ownmarker. The cell line was sensitive to various types of antitumour agents in varying degrees. It was alsosensitive to ionizing radiation. D_0 values of L_(833) and L_(833-A) cells were 98.8 and 104.9 rad, respec-tively. The results were similar to that of L_(8 相似文献
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本文用小鼠可移植性粒细胞白血病瘤株L_(801)的骨髓细胞,经体外培养建立了粒细胞白血病细胞系(株),定名为L(833)。三年来在体外已传至280余代,细胞生长迅速稳定,经不同途径动物接种致瘤率均为100％。经细胞学、细胞化学、病理学和超微结构等检查,均证明本细胞系(株)仍为粒细胞白血病细胞。L_(833)细胞系细胞染色体分析证明仍然是亚二倍体,众数39条,Y染色体丢失和存在一个标记染色体。L_(833)-A和L_(833)-B(利用半琼脂法分选出的细胞集落定名为A和B)染色体除了上述变化外,还各自存在一个标记染色体。本系细胞对所用各类抗癌药物均有不同程度的敏感性。对辐射(γ线)也很敏感,L_(833)和L_(833)-A的D_0值分别为98.8和104.9 rad。其结果和L_(801)基本是一致的,它的建立不但对于深入研究白血病和其药物筛选等有重要意义,而且利用体外瘤株进行有关研究更直观、经济和易于进行精确定量研究。 相似文献
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