非放射性多碘标记-偶合催化反应的极谱免疫分析研究

提出非放射性多碘标记-偶合催化反应的极谱免疫分析新体系,该体系以人白蛋白(HSA)作模型抗原,用含4个非放射性碘有机化合物藻红B(EB)经一步化学反应标记HSA.该标记方法简便,条件温和.所得标记物中EB:HSA摩尔比为12:1.用本方法获得的标记蛋白质含碘量远高于其它现有方法.利用标记的含碘抗原对As(Ⅲ)-Ce(Ⅳ)反应的催化作用,用极谱法检测底物中 As(Ⅲ)量变化,建立了异相竞争极谱免疫分析新方法.HSA可测浓度范围为1～200μg/mL,检测限为0.66μg/mL.提出的新体系也适用于其他蛋白质的免疫分析.     

多边形螺环链关于Hosoya指标的极值问题

Hosoya指标是化学图论研究中较为流行和重要的拓扑指标之一.首先,根据线性的、无分支的、饱和的多螺环化合物的简单分子结构图定义了"多边形螺环链";其次,研究了多边形螺环链关于Hosoya指标的极值问题;同时,得到了多边形螺环链关于Hosoya指标的排序.     

Polarographic immunoassay coupled with catalysis of non-radioactive multiple iodine label

A now polarographic immunoassay was developed In this assay,human serum albumin (HSA) as the model antigen was covalently labeled with organic compound erythrosin B(EB) containing four non-radioactive iodides through Ⅰ step chemical reaction The labeling procedure is simple and the conditions needed are moderate.The molar labeling ratio of KB HSA was 12 Ⅰ The content of iodine in the conjugate obtained by the proposed procedure is ninth higher than that by the other existing methods.A heterogeneous competitive immunoassay was established by compling the catalysis of the conjugate to substrate As(Ⅲ)-Ce(Ⅳ) reaction with the linear-sweep polarographic detec-tion of As(Ⅲ) amount HSA can be determined in the HSA concentration range from 1 to 200μg/mL,with the de-tection hum of 0 66μg/ml.     

藻红B、玫瑰红和3，5－二碘酪氨酸的催化动力学研究

藻红Ｂ、玫瑰红和３，５－二碘酪氨酸的催化动力学研究白亚丽，过玮，宋俊峰（西北大学电分析化学研究所，西安，７１００６９）关键词藻红Ｂ，玫瑰红，３，５－二碘酪氨酸，催化动力学，单扫描示波极谱法碘催化Ａｓ（３＋）－Ｃｅ（４＋）反应的现象被广泛应用于动力学测...