有机溶剂性质及其水含量对酶催化合成含D－氨基酸残基的二肽衍生物的影响

有机溶剂性质及其水含量对酶催化合成保护的寡肽及其衍生物有显著影响。系 统地研究了有机溶剂性质及在不同有机溶剂中水含量对α－胰凝乳蛋白酶催化合成 含D－氨基酸残基的二肽衍生物产率的影响。用α－胰凝乳蛋白酶、枯草杆菌蛋白 酶和嗜热杆菌蛋白酶在有机溶剂中催化合成了一系列保护的二肽及其衍生物，并研 究了水含量对反应产率的影响，得到了十分有意义的结果。     

Synthesis of Fragment Derivatives of Delta Sleep-inducing Peptide （1-4） Catalyzed by Enzymes in Organic Solvents

Synthesis of two fragment derivatives of delta sleep-inducing peptide (DSIP) (1-4), Boc-Trp-Ala-Gly-GlyNHNHPh (1) and Boc-Trp-Ala-Gly-GlyOEt (2), catalyzed by proteases via 2 2 or 1 3 synthetic routes with different protecting groups at C-terminal in organic solvents was reported. The yield of N-Boc tetrapeptide with phenylhydrazinyl protecting carboxyl group at C-terminal (1) was higher than that of N-Boc tetrapeptide ethyl ester (2) by 2 2 route. The factors influencing the synthetic yield of the fragments of DSIP (1-4) were discussed.