公共安全视域下精神药物光谱学无损分类

该文对苯二氮类和吩噻嗪类镇静安眠药样品的红外光谱数据进行采集,构建了不同数据分类模型并对比了不同波段光谱数据集对其分析准确率的影响.结果表明,借助贝叶斯判别分析的基于全波段数据集的二阶导数模型分类效果优于其他单一模型,对于苯二氮类和吩噻嗪类两个不同类型精神药物的总体分类准确率达92.7％.借助贝叶斯判别分析的基于全波段和指纹区融合数据集的二阶导数融合模型分类效果最佳,对苯二氮类和吩噻嗪类两个不同类型精神药物的总体分类准确率达到100％;对苯二氮类和吩噻嗪类中不同种类精神药物的总体分类准确率则分别达到96.7％和100％.该研究实现了不同类型及同一类型不同种类镇静安眠药的快速准确定性分析,为此类管制类药物走私案件的准确定性提供了一定的技术支持.     

Metabolic markers of the synthetic cannabinoid 5F-EMB-PICA in zebrafishEI北大核心CSCD

利用斑马鱼实验模型,借助液相色谱组合型四极杆Orbitrap质谱联用技术对N-(1-乙氧基羰基-2-甲基丙基)-1-(5-氟戊基)吲哚-3-甲酰胺(5F-EMB-PICA)的体内代谢产物进行分析,筛选潜在代谢标志物。结果表明,在斑马鱼实验模型中5F-EMB-PICA经过代谢共产生13种Ⅰ相代谢产物和6种Ⅱ相代谢产物。Ⅰ相代谢的途径包括酰胺水解、氟戊烷基侧链氧化脱氟、N-脱烷基化、羟基化和酯水解等。Ⅱ相代谢的途径主要为葡萄糖醛酸化。其中酯水解代谢产物(M5.1)响应强度最高,推荐作为5F-EMB-PICA的潜在代谢标志物。为建立生物样品中5F-EMB-PICA的标准检验方法提供相关代谢研究数据,为相关案件的检验鉴定提供技术支持。     

Determination of insulin aspart in plasma by liquid chromatography-quadrupole electrostatic field orbitrap high-resolution mass spectrometryEI北大核心CSCD

A rapid detection method for insulin aspart in human plasma was established by using liquid chromatography-quadrupole electrostatic field orbitrap high-resolution mass spectrometry. Samples were pretreated with a precipitant of methanol-acetonitrile-acetic acid solution (49:49:2, V/V/V), an Agilent Eclipse Plus C18 column (50 mm×2.1 mm, 3.5 µm) was selected, with 0.1% aqueous formic acid and 0.1% formic acid[1]acetonitrile as mobile phases for gradient elution, and mass spectrometry was performed in selected ions monitoring (SIM) scan mode. The experimental results showed that the linear relationship of insulin aspart in human plasma was good in the range of 0.1-10 µg/mL (R2=0.9938), with the limit of quantification of 0.1 µg/mL, and the limit of detection of 0.05 µg/mL. The recoveries ranged from 71.6% to 106.2%, and the relative standard deviations (RSDs) of the intra-day and inter-day were both less than 15%. The method can meet the inspection needs of actual cases. © 2023, Youke Publishing Co.,Ltd. All rights reserved.