烧伤病人血浆和痂下组织中头孢噻甲羧肟的高效液相色谱测定及其药代动力学研究

烧伤病人血浆和痂下组织中头孢噻甲羧肟的高效液相色谱测定及其药代动力学研究尤忠义，雷政宏，宗光全（第三军医大学烧伤研究所重庆６３００３８）１前言感染是烧伤病人的重要死亡原因之一￣［１］．而创面则是烧伤感染的重要来源￣［２］，抗生素是防治烧伤感染的常用药...     

烧伤病人血浆和痂下组织中头孢噻甲羧肟的高效液相色谱测定及其药代动力学研究

 A successful method for quantitative determination of ceftazidine in plasma and tissues by HPLC has beendeveloped with 10μm YWG C1 8 column of 4.6mm×250mm acetonilrile-methaiiol ( 85:15)mobile phase andUV detector dt 254nm. Average recoveries were 102. 06/for plasma and 90.09%for tissues. The detectionlimit for ceftazidine was 0.5μg/ML.     

高效液相色谱测定人体液和组织中的尿酸、黄嘌呤和次黄嘌呤

尿酸、次黄嘌呤和黄嘌呤广泛分布于生物体内,它们是形成超氧自由基(O_2~-)的酶促反应底物。     

反相高效液相色谱法测定核苷酸代谢产物

A method for the determination of high energy nucleotide′s metabolites of rabbit′s heart tissue by RP-HPLC with UltrasphereTM-C18 column at 254nm was established.The CVs were 1.4～8.8%。 The lowest de-tection limits were 6.25ng.This method is sensitive,reliable and suitable for the determination of nucleotide and it′s metabolites。