间歇电喷雾的初步研究

高灵敏度是电喷雾质谱应用最重要的需要之一[1].虽然电喷雾与基体辅助激光解吸技术的发展已经极大地提高了生物质谱的分析性能[2],但是这样的灵敏度对于许多生物样品的测试还是远远不够的,而现在采用了间歇电喷雾的方法,将灵敏度提高到了500 amol.这种质谱方法显著地改善了信噪比,提高了溶解、输出和离子化的效率,降低了样品消耗和仪器污染.间歇电喷雾的方法很容易在各种质谱仪上实现,尤其适合分子较大的蛋白质的质谱测定.     

Electrospray Lonization Fourier Transform Ion Cyclotron Resonance Mass Spectrometric Study on Sodium Azide Cluster Ions

Sodium azide has rarely been studied in gas phase or in the form of cluster ions and as a model of solid energetic substances and inorganic azide salt was ionized by electrospray ionization (ESI) and studied by high resolution Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) systematically. This paper highlights the effects of experimental conditions on the formation of salt cluster and the collision activation dissociation pathways of cluster ions to develop a microscopic understanding of inorganic azide salt clusters.     

间歇电喷雾的初步研究

设计了一种高灵敏的质谱离子化方法-间歇电喷雾，并介绍了间歇电喷雾的基本原理、稳定性和应用在蛋白质分析上的优势。这种质谱离子化方法能够显著地改善信噪比，降低对样品溶解度的要求，提高样品的离子化效率，减少样品的消耗和仪器的污染，可以预言间歇电喷雾的方法在蛋白质的分析上将会有广泛的应用前景。     

Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometric Study on Sodium Azide Cluster Ions

Introduction Mass spectrometry (MS) has been an important technique for studying size-dependent cluster properties with unparalleled sensitivity, specificity, and versatil-ity.1,2 Especially Fourier transform ion cyclotron reso-nance (FT-ICR) MS offers the benefits of ultra-high mass resolving power, superior mass accuracy, and the ability of affording useful structure information with tandem MS. The introduction of electrospray ionization (ESI) as a gentle mode of ion generation for MS…     

液相色谱/电喷雾质谱(LC/ESI/MS)在蛋白质分析鉴定中的应用

对液相色谱／电喷雾质谱（LC／ESI／MS）在蛋白质分子量测定、一级结构的分析、蛋白质和多肽纯度的鉴定、肽质量酶谱、蛋白质分子内二硫键的定量和定位、磷酰化位置的测定以及在蛋白质组中的应用等方面进行了综述和讨论。