全文获取类型
收费全文 | 73篇 |
免费 | 8篇 |
专业分类
化学 | 46篇 |
力学 | 1篇 |
数学 | 3篇 |
物理学 | 31篇 |
出版年
2024年 | 1篇 |
2023年 | 1篇 |
2020年 | 3篇 |
2019年 | 2篇 |
2018年 | 1篇 |
2017年 | 3篇 |
2016年 | 3篇 |
2015年 | 7篇 |
2014年 | 1篇 |
2013年 | 1篇 |
2012年 | 3篇 |
2011年 | 4篇 |
2010年 | 4篇 |
2007年 | 1篇 |
2006年 | 5篇 |
2005年 | 6篇 |
2004年 | 4篇 |
2002年 | 2篇 |
2000年 | 1篇 |
1999年 | 3篇 |
1997年 | 2篇 |
1996年 | 2篇 |
1995年 | 4篇 |
1994年 | 1篇 |
1993年 | 3篇 |
1991年 | 2篇 |
1990年 | 1篇 |
1977年 | 1篇 |
1976年 | 1篇 |
1973年 | 3篇 |
1972年 | 2篇 |
1958年 | 1篇 |
1914年 | 2篇 |
排序方式: 共有81条查询结果,搜索用时 15 毫秒
1.
2.
Tomá? Sára Thomas C Schwarz Dennis Kurzbach Christoph H Wunderlich Christoph Kreutz Robert Konrat 《ChemistryOpen》2014,3(3):115-123
Protein–protein interactions are of utmost importance to an understanding of biological phenomena since non-covalent and therefore reversible couplings between basic proteins leads to the formation of complex regulatory and adaptive molecular systems. Such systems are capable of maintaining their integrity and respond to external stimuli, processes intimately related to living organisms. These interactions, however, span a wide range of dissociation constants, from sub-nanomolar affinities in tight complexes to high-micromolar or even millimolar affinities in weak, transiently formed protein complexes. Herein, we demonstrate how novel NMR and EPR techniques can be used for the characterization of weak protein–protein (ligand) complexes. Applications to intrinsically disordered proteins and transiently formed protein complexes illustrate the potential of these novel techniques to study hitherto unobserved (and unobservable) higher-order structures of proteins. 相似文献
3.
Felix Nußbaumer Raphael Plangger Manuel Roeck Christoph Kreutz 《Angewandte Chemie (International ed. in English)》2020,59(39):17062-17069
We present the access to [5‐19F, 5‐13C]‐uridine and ‐cytidine phosphoramidites for the production of site‐specifically modified RNAs up to 65 nucleotides (nts). The amidites were used to introduce [5‐19F, 5‐13C]‐pyrimidine labels into five RNAs—the 30 nt human immunodeficiency virus trans activation response (HIV TAR) 2 RNA, the 61 nt human hepatitis B virus ? (hHBV ?) RNA, the 49 nt SAM VI riboswitch aptamer domain from B. angulatum, the 29 nt apical stem loop of the pre‐microRNA (miRNA) 21 and the 59 nt full length pre‐miRNA 21. The main stimulus to introduce the aromatic 19F–13C‐spin topology into RNA comes from a work of Boeszoermenyi et al., in which the dipole‐dipole interaction and the chemical shift anisotropy relaxation mechanisms cancel each other leading to advantageous TROSY properties shown for aromatic protein sidechains. This aromatic 13C–19F labeling scheme is now transferred to RNA. We provide a protocol for the resonance assignment by solid phase synthesis based on diluted [5‐19F, 5‐13C]/[5‐19F] pyrimidine labeling. For the 61 nt hHBV ? we find a beneficial 19F–13C TROSY enhancement, which should be even more pronounced in larger RNAs and will facilitate the NMR studies of larger RNAs. The [19F, 13C]‐labeling of the SAM VI aptamer domain and the pre‐miRNA 21 further opens the possibility to use the biorthogonal stable isotope reporter nuclei in in vivo NMR to observe ligand binding and microRNA processing in a biological relevant setting. 相似文献
4.
Structural principles of biomembranes 总被引:1,自引:0,他引:1
W Kreutz 《Angewandte Chemie (International ed. in English)》1972,11(7):551-567
The determination of the electron density distribution of the membrane cross sections of nerve myelin and the discs of the outer segments of retinal rods by a new X-ray evaluation method indicates, as in the case of the cross-sectional structure of the photosynthetic membrane, an “asymmetric” mass distribution. In connection with the detection of planar protein lattices in the membranes of photosynthetic bacteria, the chloroplasts of higher plants, erythrocytes, and retinal discs as well as the detection of a new category of lipid characteristics, comprising isothermal confromational and phase changes by specific ligand binding, a basis is established for the development of a general concept of biomembrane structure. 相似文献
5.
J. Jandeleit G. Urbasch H. D. Hoffmann H. -G. Treusch E. W. Kreutz 《Applied Physics A: Materials Science & Processing》1996,63(2):117-121
The ablation process of thin copper films on fused silica by picosecond laser pulses is investigated. The ablation area is characterized using optical and scanning electron microscopy. The single-shot ablation threshold fluence for 40 ps laser pulses at 1053 nm has been determinated toF
thres = 172 mJ/cm2. The ablation rate per pulse is measured as a function of intensity in the range of 5 × 109 to 2 × 1011 W/cm2 and changes from 80 to 250 nm with increasing intensity. The experimental ablation rate per pulse is compared to heat-flow calculations based on the two-temperature model for ultrafast laser heating. Possible applications of picosecond laser radiation for microstructuring of different materials are discussed. 相似文献
6.
7.
8.
R. Weichenhain A. Horn E.W. Kreutz 《Applied Physics A: Materials Science & Processing》1999,69(7):S855-S858
Nano- and picosecond laser radiation offers better possibilities by achieving higher accuracy in microstructuring because of much lower heat load compared to larger pulses. The influence of wavelength, pulse energy, overlap and the properties of processing gas atmosphere on material removal and roughness of ceramics are investigated for ns-pulses. The removal threshold and the removal rate per pulse for ns- and ps- laser pulses are presented. Strategies, which reduce the surface roughness and enhance the efficieny of the production of three dimensional structures are reported. These results show the parameter field which permits a precise material removal during the production of microstructures without damaging the remaining structure. 相似文献
9.
Dr. Samuel M. Meier M. Sc. Dominique Kreutz Dr. Lilli Winter M. Sc. Matthias H. M. Klose M. Sc. Klaudia Cseh M. Sc. Tamara Weiss Dr. Andrea Bileck M. Sc. Beatrix Alte Dr. Johanna C. Mader M. Sc. Samir Jana Dr. Annesha Chatterjee Dr. Arindam Bhattacharyya Michaela Hejl Dr. Michael A. Jakupec Priv.-Doz. Dr. Petra Heffeter Prof. Dr. Walter Berger Prof. Dr. Christian G. Hartinger Prof. Dr. Bernhard K. Keppler Prof. Dr. Gerhard Wiche Prof. Dr. Christopher Gerner 《Angewandte Chemie (International ed. in English)》2017,56(28):8267-8271
Organometallic metal(arene) anticancer agents require ligand exchange for their anticancer activity and this is generally believed to confer low selectivity for potential cellular targets. However, using an integrated proteomics-based target-response profiling approach as a potent hypothesis-generating procedure, we found an unexpected target selectivity of a ruthenium(arene) pyridinecarbothioamide (plecstatin) for plectin, a scaffold protein and cytolinker, which was validated in a plectin knock-out model in vitro. Plectin targeting shows potential as a strategy to inhibit tumor invasiveness as shown in cultured tumor spheroids while oral administration of plecstatin-1 to mice reduces tumor growth more efficiently in the invasive B16 melanoma than in the CT26 colon tumor model. 相似文献
10.
U. Milkau M. L. Begemann-Blaich E. -M. Eckert G. Imme P. Kreutz A. Kühmichel M. Lattuada U. Lynen C. Mazur W. F. J. Müller J. B. Natowitz C. Ngô J. Pochodzalla G. Raciti M. Ribrag H. Sann W. Trautmann R. Trockel 《Zeitschrift für Physik A Hadrons and Nuclei》1993,346(3):227-236
Inclusive cross sections of intermediate mass fragments from the reaction84Kr+197Au atE/A=35 MeV were measured over the range 8°≦Θ lab≦70° with a low detection threshold. A moving-source parameterization was used to fit the double-differential cross sections. The integrated cross section for fragment production exceeds the total reaction cross section thus indicating a large probability for multi-fragment processes. The deduced large temperature parameters can be explained by assuming emission from a rotating source. From the comparison to reactions with12C and40Ar projectiles at E/A=30 MeV a systematics of inclusive fragment production as a function of the projectile mass is obtained. 相似文献