全文获取类型
收费全文 | 107篇 |
免费 | 2篇 |
国内免费 | 3篇 |
专业分类
化学 | 67篇 |
力学 | 1篇 |
数学 | 19篇 |
物理学 | 25篇 |
出版年
2023年 | 1篇 |
2022年 | 1篇 |
2021年 | 6篇 |
2020年 | 1篇 |
2019年 | 1篇 |
2017年 | 4篇 |
2016年 | 2篇 |
2015年 | 2篇 |
2014年 | 2篇 |
2013年 | 2篇 |
2012年 | 4篇 |
2011年 | 1篇 |
2010年 | 1篇 |
2009年 | 1篇 |
2008年 | 11篇 |
2007年 | 6篇 |
2006年 | 13篇 |
2005年 | 6篇 |
2004年 | 2篇 |
2003年 | 9篇 |
2002年 | 4篇 |
2001年 | 5篇 |
2000年 | 1篇 |
1999年 | 1篇 |
1998年 | 3篇 |
1996年 | 3篇 |
1995年 | 2篇 |
1993年 | 1篇 |
1990年 | 2篇 |
1988年 | 1篇 |
1987年 | 2篇 |
1985年 | 3篇 |
1984年 | 1篇 |
1983年 | 1篇 |
1982年 | 1篇 |
1980年 | 2篇 |
1979年 | 1篇 |
1977年 | 1篇 |
1976年 | 1篇 |
排序方式: 共有112条查询结果,搜索用时 46 毫秒
1.
2.
Thereza Christina Vessoni Penna Marina Ishii Adalberto Pessoa Junior Laura de Oliveira Nascimento Luciana Cambricoli de Souza Olivia Cholewa 《Applied biochemistry and biotechnology》2004,114(1-3):453-468
To determine the influence of various culture conditions, transformed cells of Escherichia coli expressing recombinant green fluorescent protein (GFPuv) were grown in nine cultures with four variable conditions (storage
of inoculated broth at 4°C prior to incubation, agitation speed, isopropyl-β-d-thiogalactopyranoside [IPTG] concentration, and induction time). The pelleted cells were resuspended in extraction buffer
and subjected to the three-phase partitioning (TPP) extraction method. To determine the most appropriate purification resin,
protein extracts were eluted through one of four types of HiTrap hydrophobic interaction chromatography (HIC) columns prepacked
with methyl, butyl, octyl, or phenyl resins and analyzed further on a 12% sodium dodecylsulfatepolyacrylamidegel. With Coomassie
staining, a single band between 27 (standard GFPuv) and 29 kDa (molecular weight standard) was visualized for every HIC column
sample. TPP extraction with HIC elution provided about 90% of the GFPuv recovered and eight-fold GFPuv enrichment related
to the specific mass. Rotary speed and IPTG concentration showed, respectively, greater negative and positive influences on
GFPuv expression at the beginning of the logarithmic phase for the set culture conditions (37°C, 24-h incubation). 相似文献
3.
Thereza Christina Vessoni Penna Eb Chiarini Adalberto Pessoa Junior 《Applied biochemistry and biotechnology》2003,107(1-3):481-491
Transformed cells of Escherichia coli expressing recombinant green fluorescent protein (GFPuv) were subjected to two methods of extraction: (1) freezing/thawing/sonication
(FTS) cycles prior to the three-phase partitioning (TPP) method, or (2) directly to TPP extraction. The amount of GFPuv released
by the FTS plus TPP method varied: 374μg/mL (first cycle), 93–442 μg/mL (second cycle), 32–359 μg/mL (third cycle), 18–115
μg/mL (fourth cycle). The GFPuv yields by the second method (TPP only) were, 23–54 μg/mL for the first extract and 33–91 μg/mL
for the second. The FTS plus TPP method released similar amounts of GFPuv to that extracted by TPP; and provided a better
mixture elution through the hydrophobic interaction column: 13–63 μg/mL for FTS plus TPP methods, and 2.5–13 μg/mL for TPP.
The results showed that although selective permeation is a more laborious methodology, it was more efficient for obtaining
of GFPuv in relation to the direct extraction of the cells for TPP. 相似文献
4.
Elenise Bannwart de Moraes Patricia Fazzio Martins César Benedito Batistella Mario Eusebio Torres Alvarez Rubens Maciel Filho Maria Regina Wolf Maciel 《Applied biochemistry and biotechnology》2006,132(1-3):1066-1076
Molecular distillation was studied for the separation of tocopherols from soya sludge, both experimentally and by simulation,
under different operating conditions, with good agreement. Evaporator temperatures varied from 100°C to 160°C and feed flow
rates ranged from 0.1 to 0.8 kg/h. The process pressure was maintained at 10−6 bar, the feed temperature at 50°C, the condenser temperature at 60°C, and the stirring at 350 rpm. For each process condition,
samples of both streams (distillate and residue) were collected and stored at −18°C before tocopherols analyses. Owing to
the differences between molecular weights and vapor pressures of free fatty acids and tocopherols, tocopherols preferentially
remained in the residue at evaporator temperatures of 100°C and 120°C, whereas for higher temperatures (140°C and 160°C) and
lower feed flow rate, tocopherols tended to migrate to the distillate stream. 相似文献
5.
The determination of safe sampling volumes of benzene and acetone on air samplers filled with Porapak Q, Chromosorb 101, 102 and 103 is described. Two direct methods are employed; one method is based on frontal chromatography, while the second one is a concentration method followed by thermal desorption. The results are compared to those determined by the indirect pulse chromatographic method. The safe sampling volumes determined by the direct methods were in accordance. The indirect method gives slightly different values; however, this procedure is carried out at "infinite dilution". The effect of the pollutant concentration on the safe sampling volumes was also examined. The values decrease with increasing pollutant concentration. 相似文献
6.
Alexsandra Conceição Apolinário Antonio Diogo Silva Vieira Susana Marta Isay Saad Adalberto Pessoa Jr José Alexsandro da Silva 《Natural product research》2020,34(16):2367-2371
Abstract This work aimed at evaluating the prebiotic potential of the aqueous extract and crude polysaccharides from Agave sisalana boles by an in vitro screening. Crude polysaccharides were obtained from the aqueous bole extract by precipitation with acetone and resuspension in water. The liquid extract and the polysaccharide solution were then spray dried and submitted to thermal analysis and quantification of metabolites. Prebiotic activity was checked on probiotic strains belonging to the Lactobacillus genus using inulin, fructo-oligosaccharides, fructose and glucose as positive controls. The powder of A. sisalana bole extract, which has recently been identified as a rich source of inulin, exhibited higher potential of fermentation compared with crude polysaccharides. 相似文献
7.
8.
Formulation of Aluminum Chloride Phthalocyanine in Pluronic™ P‐123 and F‐127 Block Copolymer Micelles: Photophysical properties and Photodynamic Inactivation of Microorganisms 下载免费PDF全文
Bruno Henrique Vilsinski Adriana Passarella Gerola Junior Adalberto Enumo Katieli da Silva Souza Campanholi Paulo Cesar de Souza Pereira Gustavo Braga Noboru Hioka Elza Kimura André Luiz Tessaro Wilker Caetano 《Photochemistry and photobiology》2015,91(3):518-525
Aluminum Chloride Phthalocyanine (AlPcCl) can be used as a photosensitizer (PS) for Photodynamic Inactivation of Microorganisms (PDI). The AlPcCl showed favorable characteristics for PDI due to high quantum yield of singlet oxygen (ΦΔ) and photostability. Physicochemical properties and photodynamic inactivation of AlPcCl incorporated in polymeric micelles of tri‐block copolymer (P‐123 and F‐127) against microorganisms Staphylococcus aureus, Escherichia coli and Candida albicans were investigated in this work. Previously, it was observed that the AlPcCl undergoes self‐aggregation in F‐127, while in P‐123 the PS is in a monomeric form suitable for PDI. Due to the self‐aggregation of AlPcCl in F‐127, this formulation did not show any effect on these microorganisms. On the other hand, AlPcCl formulated in P‐123 was effective against S. aureus and C. albicans and the death of microorganisms was dependent on the PS concentration and illumination time. Additionally, it was found that the values of PS concentration and illumination time to eradicate 90% of the initial population of microorganisms (IC90 and D90, respectively) were small for the AlPcCl in P‐123, showing the effectiveness of this formulation for PDI. 相似文献
9.
Existence and Regularity of Periodic Solutions to Certain First-Order Partial Differential Equations
Adalberto?P.?BergamascoEmail author Paulo?L.?Dattori da Silva Rafael?B.?Gonzalez 《Journal of Fourier Analysis and Applications》2017,23(1):65-90
We present conditions on the coefficients of a class of vector fields on the torus which yield a characterization of global solvability as well as global hypoellipticity, in other words, the existence and regularity of periodic solutions. Diophantine conditions and connectedness of certain sublevel sets appear in a natural way in our results. 相似文献
10.
A. C. Pavto M. Braga A. Fazzio J. R. Leite 《International journal of quantum chemistry》1980,18(5):1165-1173
An exchange-correlation potential model to be used in connection with the multiple scattering method is presented. Retaining the main advantages of the multiple scattering method with the Xα potential, particularly its low computational requirements, this new formalism does not require any adjustable parameter. Test calculations on the NiF64? system are reported and compared with experimental and ab initio results. 相似文献