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The increased interest in fish skin collagen results from the risk of BSE disease and the production of native collagen by the acidic hydration method. As a material, FSC derived by the novel acidic hydration method was used. The bovine Achilles tendon (BAT) collagen type I was applied as a control material. Measurements were carried out at the temperature range 2980 K-510 K. Each sample was heated twice. During the first heating run for FSC, to 380 K, the maximum was localized at 343 K, at electrical conductivity of 7 · 10−6 S/m. In the second heating run to 510 K, the maximum was observed at 443 K for FSC, and at 487 K for BAT collagen. The peaks revealed in the temperature range 320-350 K were related to the free water and bound water release, alike for FSC and BAT collagen. The process of water removal for both types of collagen takes place in similar temperature ranges. 相似文献
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Marlena Pięta Jacek Kędzia Dorota Kowalczyk Jakub Wojciechowski Wojciech M. Wolf Tomasz Janecki 《Tetrahedron》2019,75(17):2495-2505
An efficient, versatile, enantioselective synthesis of 1,3-disubstituted and 1,3,6-trisubstituted 5-methylidenedihydrouracils applying Horner-Wadsworth Emmons methodology was developed. Starting 1,3-disubstituted 5-diethoxyphosphoryluracils were subjected to reduction of the double bond or addition of various Grignard reagents and obtained Horner-Wadsworth Emmons reagents were used for the olefination of formaldehyde. Enantioselective synthesis of 1,3,6-trisubstituted 5-methylidenedihydrouracils was accomplished by introducing (R,R)- or (S,S)-di(1-phenylethylamino)phosphoryl groups as chiral auxiliary. Additions of Grignard reagents in the presence of these groups were highly and complimentary diastereoselective (de?~?80%). Further separation of the diastereomeric mixtures by column chromatography enabled synthesis of (R)- and (S)-1,3,6-trisubstituted-5-methylidenedihydrouracils with ee?≥?98%. Furthermore, absolute configuration of the adducts and final products was established using single crystal X-ray analysis. Stereochemical course of the addition reactions is also discussed. 相似文献
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Paulina Grocholska Andrzej Konieczny Zuzanna Ka
mierczak Krystyna Dbrowska Karolina Panek-Laszczyska Marlena Kak Wojciech Witkiewicz Zbigniew Szewczuk Remigiusz Bchor 《Molecules (Basel, Switzerland)》2021,26(23)
Early detection of any preeclampsia biomarkers may lower the risk of mortality, both for a mother and a child. Our study focuses on techniques for preeclampsia biomarker identification by comparing the results of a method using liquid chromatography mass spectrometry in multiple reaction monitoring mode (LC-MS/MS) with those by the enzyme-linked immunosorbent assay (ELISA) test, as well as by comparing the obtained results with clinical data. In the proposed LC-MS/MS method a tryptic digest peptide charge derivatization strategy was used as a tool for sensitive detection of podocin, i.e., a previously discovered preeclampsia biomarker present in urine samples from pregnant women. Urine samples from pregnant women with diagnosed preeclampsia were collected at different stages of pregnancy and from healthy subjects, and then were analyzed by ELISA test and the proposed method with LC-MS/MS. Charge derivatization of the ε amino group of C-terminal lysine residues in tryptic digests by 2,4,6-triphenylpyrylium salt was performed to increase the ionization efficiency in the LC-MS/MS mode. Podocin was identified at the early stage of pregnancy, while its detection using an ELISA test was not possible. The protocol for urine sample preparation was optimized. Our results show that the proposed method by LC-MS/MS in combination with peptide charge derivatization, provides an ultrasensitive tool for diagnosis of preeclampsia, and provides earlier detection than a clinical diagnosis or ELISA test. The proposed solution may revolutionize medical diagnostics. 相似文献
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Tran BL Washington MP Henckel DA Gao X Park H Pink M Mindiola DJ 《Chemical communications (Cambridge, England)》2012,48(10):1529-1531
Treatment of d(1) [(nacnac)TiCl(Ntol(2))] with NaN(3) results in NaCl formation and N(2) ejection to yield the first four coordinate, parent imide [(nacnac)Ti=NH(Ntol(2))] (nacnac(-)=[ArNC(CH(3))](2)CH, Ar = 2,6-iPr(2)C(6)H(3), tol = 4-CH(3)C(6)H(4)). 相似文献
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Joanna Wojnarska Katarzyna Ostrowska Marlena Gryl Katarzyna Marta Stadnicka 《Acta Crystallographica. Section C, Structural Chemistry》2019,75(9):1228-1233
The carboxylic acid group is an example of a functional group which possess a good hydrogen‐bond donor (–OH) and acceptor (C=O). For this reason, carboxylic acids have a tendency to self‐assembly by the formation of hydrogen bonds between the donor and acceptor sites. We present here the crystal structure of N‐tosyl‐l ‐proline (TPOH) benzene hemisolvate {systematic name: (2S)‐1‐[(4‐methylbenzene)sulfonyl]pyrrolidine‐2‐carboxylic acid benzene hemisolvate}, C12H15NO4S·0.5C6H6, (I), in which a cyclic R22(8) hydrogen‐bonded carboxylic acid dimer with a strong O—(H)…(H)—O hydrogen bond is observed. The compound was characterized by single‐crystal X‐ray diffraction and NMR spectroscopy, and crystallizes in the space group I2 with half a benzene molecule and one TPOH molecule in the asymmetric unit. The H atom of the carboxyl OH group is disordered over a twofold axis. An analysis of the intermolecular interactions using the noncovalent interaction (NCI) index showed that the TPOH molecules form dimers due to the strong O—(H)…(H)—O hydrogen bond, while the packing of the benzene solvent molecules is governed by weak dispersive interactions. A search of the Cambridge Structural Database revealed that the disordered dimeric motif observed in (I) was found previously only in six crystal structures. 相似文献
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Marlena Pięta Jacek Kędzia Jakub Wojciechowski Tomasz Janecki 《Tetrahedron: Asymmetry》2017,28(4):567-576
A series of enantiomerically pure or highly enriched (R)- or (S)-3-methylidenetetrahydroquinolin-2-ones was readily prepared by highly diastereoselective Michael additions of various Grignard reagents to quinolin-2(1H)-ones, containing an (R,R)- or (S,S)-di(1-phenylethylamino)phosphoryl group as chiral auxiliary, followed by Horner-Wadsworth-Emmons olefination of formaldehyde. An efficient synthesis of the starting (R,R)- and (S,S)-3-({di[(1-phenylethyl)amino]}phosphoryl)-1-alkyl-quinolin-2(1H)-ones is also described. The relative and absolute configurations of the intermediate adducts and final methylidenequinolinones were established by NMR and X-ray analysis. 相似文献
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Agnieszka Pawlos Marlena Broncel Ewelina Wo
niak Paulina Gorzelak-Pabi 《Molecules (Basel, Switzerland)》2021,26(23)
Patients with diabetes are at higher risk of cardiovascular diseases and cognitive impairment. SGLT2 inhibitors (Empagliflozin, Canagliflozin, Dapagliflozin, Ertugliflozin, Sotagliflozin) are newer hypoglycemic agents with many pleiotropic effects. In this review, we discuss their neuroprotective potential. SGLT2 inhibitors (SGLT2i) are lipid-soluble and reach the brain/serum ratio from 0.3 to 0.5. SGLT receptors are present in the central nervous system (CNS). Flozins are not fully SGLT2-selective and have an affinity for the SGLT1 receptor, which is associated with protection against ischemia/reperfusion brain damage. SGLT2i show an anti-inflammatory and anti-atherosclerotic effect, including reduction of proinflammatory cytokines, M2 macrophage polarization, JAK2/STAT1 and NLRP3 inflammasome inhibition, as well as cIMT regression. They also mitigate oxidative stress. SGLT2i improve endothelial function, prevent remodeling and exert a protective effect on the neurovascular unit, blood-brain barrier, pericytes, astrocytes, microglia, and oligodendrocytes. Flozins are also able to inhibit AChE, which contributes to cognitive improvement. Empagliflozin significantly increases the level of cerebral BDNF, which modulates neurotransmission and ensures growth, survival, and plasticity of neurons. Moreover, they may be able to restore the circadian rhythm of mTOR activation, which is quite a novel finding in the field of research on metabolic diseases and cognitive impairment. SGLT2i have a great potential to protect against atherosclerosis and cognitive impairment in patients with type 2 diabetes mellitus. 相似文献
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