Effects of CRAC Channel on Spatiotemporal Ca2＋ Patterns in T Cells

A reaction-diffusion model is built to investigate the temporal and spatial patterns of cytoplasmic Ca2＋ dynamics under the effects of Ca2＋-release activated Ca2＋ （CRAC） channels in T cells. Simulation results show a strong dependence of the modulation mode of Ca2＋ oscillation and dynamic patterns of Ca2＋ wave on the influx rate through the CRAC channel （ksoc）. When ksoc is small, cytoplasmic Ca2＋ is modulated as a frequency-modulation （FM） signal, whereas it shows an amplitude modulation （AM） mode after ksoc passes through a critical value. The heterogeneity in spatial Ca2＋ distribution is mostly arising from the influx through CRAC channels in both FM and AM modes. During each Ca2＋ spike, a more sustained cytoplasmic Ca2＋ gradient is maintained in the AM mode rather than in the FM mode.     

果蝇昼夜生理节律之细胞质PER-TIM间隔定时器的大聚合物模型

昼夜生理节律使得生物体的生理和行为能够适应外界环境的昼夜循环变化. 实验发现在果蝇的细胞质中存在由PER-TIM复合体组成的间隔计时器. 本文提出了这个间隔定时器的一种可能的数学模型. 模型的特点是大聚合物, 快速交换, 不同磷酸化位点有不同功能. 模拟结果基本与实验相符, 符合PER-TIM间隔定时器的主要时间特征并重现了per^L 变异细胞系中定时器的特点, 以及PER和TIM蛋白进入细胞核的三种不同类型的过程.     

Cytoplasmic Ca2＋ Dynamics under the Interplay between the Different IP3R Gating Models and the Plasma Membrane Ca2＋ Influx

The cytoplasmic Ca2＋ oscillations are investigated under the effect of CRAC channels in non-excitable cells （especially in T cells）. The oscillatory Ca2＋ signals can be modulated as the amplitude-, frequency- and mixed amplitude-frequency modulation modes dependent on the different IP3R gating models. Bifurcation analyses show that Ca2＋ signals in the single positive feedback loop model is a mixed modulation mode. In contrast, Ca2＋ signals in the Mak-McBride-Foskett model demonstrates approximately the frequency modulation mode only with slight amplitude shifts.