Two Coordination Polymers: Protective Activity On Nerve Injury by Increasing miR-219 and Reducing Inflammatory Response

Two novel transition metal coordination polymers (CPs) of {Cu(tdc)(Hdmea)₂ (1, Hdmea?=?N,N-dimethylethanolamine, tdc?=?2,5-thiopenedicarboxylate) and [Co₃(H₂O)₃(tdc)₃(dioxane)]·2(dioxane) (2) were prepared via the reaction of Co(NO₃)₂·6H₂O or Cu(NO₃)₂·3H₂O and H₂tdc with or without the template reagent Hdmea. The following experiments were carried out to determine the treatment activity for compounds against nerve injury. Firstly, the real time RT-PCR was carried out to determine the miR-219 relative expression in the nerve cells. Then the activation level of the AMPK signaling pathway in the nerve cells was measured with western blot. In addition to this, the ELISA detection was also used in this research, for the inflammatory cytokines’ evaluation. Finally, the inhibition of the compound 1 and compound 2 on the death of the nerve cells was determined with MTT assay.