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1.
G. Kuncova O. Podrazky S. Ripp J. Trögl G.S. Sayler K. Demnerova R. Vankova 《Journal of Sol-Gel Science and Technology》2004,31(1-3):335-342
Three different types of cells, Pseudomonas fluorescens HK44, Saccharomyces cerevisiae strain SP4 and plant cells Nicotiana tabacum L. BY-2, were immobilized by entrapment in tetramethoxysilane prepolymer (TMOS) gel or in composite gel containing prepolymer TMOS and alginate in various ratios. Their growth and viability were monitored by bioluminescence and 2-D fluorescence spectra, which are fast and do not need the dissolution of a matrix. The resulting biocomposite gels were obtained by gelation of the mixtures of TMOS prep. or TMOS/alginate sols and the particular cells in proper media on glass supports to provide films ~1 mm thick. The effect of the following parameters on the growth and viability of the cells was studied: (a) the composition of the biocomposites, (b) the preparation conditions of TMOS and (c) the conditions of the procedure of entrapment. All three types of cells were tested in TMOS gel and the composite TMOS/alginate = 1:1 (v/v). The sensitivity of the cells to the changes of conditions increased in the sequence: P. fluorescence HK44 < S. cerevisiae strain SP4 < N. tabacum L. BY-2. Cell viability decreased with the increasing content of Si in biocomposites. The entrapment into alginate–silica composites resulted in the leakage of microbial and yeast cells. However, it had positive effects on the growth and metabolic activity of plant cells. 相似文献
2.
Burlage Robert S. Palumbo Anthony V. Heitzer Armin Sayler Gary 《Applied biochemistry and biotechnology》1994,45(1):731-740
Applied Biochemistry and Biotechnology - Reporter strains of bacteria were tested using soil samples from several sites near a leaking fuel oil storage facility. The reporter bacteria utilized the... 相似文献
3.
Digrazia Philip M. Blackburn James W. Bienkowski Paul R. Hilton Barry Reed Gregory D. King J. M. Henry Sayler Gary S. 《Applied biochemistry and biotechnology》1990,(1):237-252
An experimental and mathematical method is developed for the microbial systems analysis of polyaromatic hydrocarbon (PAH)-degrading
mixed cultures in PAH-contaminated “town gas” soil systems. Frequency response is the primary experimental and data analysis
tool used to probe the structure of these complicated systems. The objective is to provide a fundamental protocol for evaluating
the performance of specific mixed microbial cultures on specific soil systems by elucidating the salient system variables
and their interactions. Two well-described reactor systems, a constant volume stirred tank reactor (CSTR) and a plug flow
differential volume reactor, are used in order to remove performance effects that are related to reactor type as opposed to
system structure. These two reactor systems are well-defined systems that can be described mathematically and represent the
two extremes of one potentially important system variable, macroscopic mass transfer. The experimental and mathematical structure
of the protocol is described, experimental data is presented, and data analysis is demonstrated for the stripping, sorption,
and biodegradation of napththalene. 相似文献
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We have measured and explained a new mechanism of molecular ionization near the appearance intensity that produces a sequence of peaks in the nuclear kinetic energy spectrum separated by the photon energy. Our interpretation is based on an internally consistent model for the nuclear motion during an intense laser pulse. Within this model, the same concepts and language can be used for both dissociation and ionization, leading to a more unified understanding of the dynamics. 相似文献
6.
McKenna J Sayler AM Anis F Gaire B Johnson NG Parke E Hua JJ Mashiko H Nakamura CM Moon E Chang Z Carnes KD Esry BD Ben-Itzhak I 《Physical review letters》2008,100(13):133001
High-order (three-photon or more) above-threshold dissociation (ATD) of H(2)(+) has generally not been observed using 800 nm light. We demonstrate a strong enhancement of its probability using intense 7 fs laser pulses interacting with beams of H(2)(+), HD(+), and D(2)(+) ions. The mechanism invokes a dynamic control of the dissociation pathway. These measurements are supported by theory that additionally reveals, for the first time, an unexpectedly large contribution to ATD from highly excited electronic states. 相似文献
7.
Laroussi M. Sayler G.S. Glascock B.B. McCurdy B. Pearce M.E. Bright N.G. Malott C.M. 《IEEE transactions on plasma science. IEEE Nuclear and Plasma Sciences Society》1999,27(1):34-35
Among the various industrial uses of the glow discharge at atmospheric pressure (GDAP), biological applications such as sterilization are under investigation. In this paper, we present images of a liquid medium (Luria-Bertani broth with tetracycline) contaminated by Escherichia coli bacteria (strain PER 322) undergoing plasma treatment. In most cases, it is found that an exposure time of two to 20 minutes leads to nearly a complete kill of a 108/ml E. coli population. The treatment time necessary to obtain a complete kill depends on the plasma power density, the type of gas used, the type of bacteria, and the type of medium 相似文献
8.
Smartt AE Xu T Jegier P Carswell JJ Blount SA Sayler GS Ripp S 《Analytical and bioanalytical chemistry》2012,402(10):3127-3146
Bacteriophages, or phages, are bacterial viruses that can infect a broad or narrow range of host organisms. Knowing the host
range of a phage allows it to be exploited in targeting various pathogens. Applying phages for the identification of microorganisms
related to food and waterborne pathogens and pathogens of clinical significance to humans and animals has a long history,
and there has to some extent been a recent revival in these applications as phages have become more extensively integrated
into novel detection, identification, and monitoring technologies. Biotechnological and genetic engineering strategies applied
to phages are responsible for some of these new methods, but even natural unmodified phages are widely applicable when paired
with appropriate innovative detector platforms. This review highlights the use of phages as pathogen detector interfaces to
provide the reader with an up-to-date inventory of phage-based biodetection strategies. 相似文献
9.
Ripp S Jegier P Johnson CM Brigati JR Sayler GS 《Analytical and bioanalytical chemistry》2008,391(2):507-514
Escherichia coli O157:H7 remains a continuous public health threat, appearing in meats, water, fruit juices, milk, cheese, and vegetables,
where its ingestion at concentrations of perhaps as low as 10 to 100 organisms can result in potent toxin exposure and severe
damage to the lining of the intestine. Abdominal pain and diarrhea develop, which in the very young or elderly can progress
towards hemolytic uremic syndrome and kidney failure. To assist in the detection of E. coli O157:H7, a recombinant bacteriophage reporter was developed that uses quorum sensing (luxI/luxR) signaling and luxCDABE-based bioluminescent bioreporter sensing to specifically and autonomously respond to O157:H7 serotype E. coli. The bacteriophage reporter, derived from phage PP01, was tested in artificially contaminated foodstuffs including apple
juice, tap water, ground beef, and spinach leaf rinsates. In apple juice, detection of E. coli O157:H7 at original inoculums of 1 CFU mL−1 occurred within approximately 16 h after a 6-h pre-incubation, detection of 1 CFU mL−1 in tap water occurred within approximately 6.5 h after a 6-h pre-incubation, and detection in spinach leaf rinsates using
a real-time Xenogen IVIS imaging system resulted in detection of 1 CFU mL−1 within approximately 4 h after a 2-h pre-incubation. Detection in ground beef was not successful, however, presumably due
to the natural occurrence of quorum sensing autoinducer (N-3-(oxohexanoyl)-l-homoserine lactone; OHHL), which generated false-positive bioreporter signals in the ground beef samples. 相似文献
10.
Heiko Schmidt Alan R. Kerstein Scott Wunsch Renaud Nédélec Ben J. Sayler 《Theoretical and Computational Fluid Dynamics》2013,27(3-4):377-395
Numerical simulations using the one-dimensional-turbulence (ODT) model are compared to water-tank measurements emulating convection and entrainment in stratiform clouds driven by cloud-top cooling. Measured dependences of the entrainment rate on Richardson number were numerically reproduced for water trials in which the initial stratification is due to temperature differences. For an additional set of trials where the initial stratification is obtained by adding dextrose to the lower layer of the tank, measured dependences of the entrainment rate on Richardson number were partially reproduced, and importantly, the model also captures the measured sensitivity of entrainment to molecular transport. Additional parameter variations suggest other dependences of the entrainment rate. Analysis suggests possible qualitative differences between laboratory and cloud entrainment behaviors that might be testable using ODT. 相似文献