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Penna Thereza Christina Vessoni Marques Marcelo Machoshvili Irena A. Ishii Marina 《Applied biochemistry and biotechnology》2002,98(1-9):539-551
Large-volume parenteral solutions were submitted to heat treatments after being inoculated with Bacillus stearothermophilus ATCC 7953 (T
r
=121°C) and Bacillus subtilis ATCC 9372 (T
r
=104.5°C) spores. The average decimal reduction time for B. stearothermophilus ranged from a D
121°C value of 1.31 to 3.14 min, in glucophysiologic and Ringer’s solutions respectively. For B. subtilis, D
104.5°C value increased from 0.69 to 1.37 min, in Ringer’s (pH=5.91) and 50% glucose (pH 3.05) solutions respectively. The z value ranged from 7.95°C (20% mannitol solution) to 13.14°C (50% glucose solution), corresponding to an activation energy
(Ea) of 81.48 and 49.30 kcal/mol, respectively. 相似文献
2.
Penna Thereza Christina Vessoni Ishii Marina Machoshvili Irene Alexeevna Marques Marcelo 《Applied biochemistry and biotechnology》2002,98(1-9):525-538
Paper strips inoculated with spores of Bacillus stearothermophilus ATCC 7953 were conventionally dried (lot 1) and lyophilized (lot 2); stored in defined environments of 32 and 86% relative
humidity at 10, 25 and 33°C for 210 d; and submitted to moist heat treatments at 121°C. A significant decrease in thermal
resistance from initial starting levels was found for lyophilized bioindicators stored at 86% relative humidity. The respective
average D
121°C values were 1.55 ± 0.05 and 1.37 ± 0.10 min for lyophilized bioindicators stored at 32 and 86% relative humidity; and 1.65±0.15min
and 1.57 ± 0.11 min for dried bioindicators stored in the same environments. 相似文献
3.
Penna Thereza Christina Vessoni Chiarini Eb Machoshvili Irene Alexeevna Ishii Marina Pessoa Adalberto 《Applied biochemistry and biotechnology》2002,98(1-9):791-802
The recombinant green fluorescent protein (gfp
uv
) was expressed by Escherichia coli DH5-α cells transformed with the plasmid pGFPuv. The gfp
uv
was selectively permeabilized from the cells in buffer solution (25 mM Tris-HCl, pH 8.0), after freezing (−70°C for 15 h), by four freeze (−20°C)/thaw cycles interlaid by sonication. The average
content of released gfp
uv
(experiment 2) was 7.76, 34.58, 39.38, 12.90, and 5.38%, for the initial freezing (−70°C) and the first, second, third and
fourth freeze/thaw cycles, respectively. Superfusion on freezing was observed between −11°C and −14°C, after which it reached
−20°C at 0.83°C/min. 相似文献
4.
Thereza Christina Vessoni Penna Irene A. Machoshvili Marina Ishii 《Applied biochemistry and biotechnology》2003,106(1-3):287-294
The interference of eight components in the yield of sporulation and thermal resistance to moist heat (121°C) of Bacillus stearothermophilus spores suspended in 0.02 M calcium acetate solution and inoculated on paper strips previously treated with calcium acetate/calcium hydroxide was studied.
The spore yield of 1.0×108/mL was developed at 62°C in 17 media containing different concentrations of d-glucose, sodium chloride, l-glutamic acid, yeast extract, peptone, manganese sulfate, potassium phosphate, and ammonium phosphate. The combined effects
of yeast extract, peptone, and glucose contributed positively to the spore yield and to the stability of the thermal resistance
of both spores in suspension and on strips. 相似文献
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