Effect of media on spore yield and thermal resistance of Bacillus stearothermophilus |
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| Authors: | Thereza Christina Vessoni Penna Irene A Machoshvili Marina Ishii |
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| Institution: | 1. Department of Biochemical and Pharmaceutical Technology, School of Pharmaceutical Science, University of S?o Paulo, Rua Antonio de Macedo Soares, 452 04607-000, S?o Paulo/SP, Brazil
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| Abstract: | The interference of eight components in the yield of sporulation and thermal resistance to moist heat (121°C) of Bacillus stearothermophilus spores suspended in 0.02 M calcium acetate solution and inoculated on paper strips previously treated with calcium acetate/calcium hydroxide was studied.
The spore yield of 1.0×108/mL was developed at 62°C in 17 media containing different concentrations of d-glucose, sodium chloride, l-glutamic acid, yeast extract, peptone, manganese sulfate, potassium phosphate, and ammonium phosphate. The combined effects
of yeast extract, peptone, and glucose contributed positively to the spore yield and to the stability of the thermal resistance
of both spores in suspension and on strips. |
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| Keywords: | Bacillus stearothermophilus thermoresistance sporulation bioindicator |
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