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Applied Biochemistry and Biotechnology - 相似文献
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Simms PJ Jeffries CT Huang Y Zhang L Arrhenius T Nadzan AM 《Journal of combinatorial chemistry》2001,3(5):427-433
A micellar electrokinetic chromatography (MEKC) method has been developed that can evaluate the purity of samples generated in combinatorial chemistry libraries. This method uses an open tube capillary (27 cm x 50 microm) along with a run buffer composed of sodium dodecyl sulfate (SDS), hydroxypropyl-beta-cyclodextrin, and sodium tetraborate coupled with UV detection. Neutral compounds and compounds that were insoluble in aqueous buffers could be analyzed under these conditions in approximately 3 min. The concentration of SDS and the concentration of hydroxypropyl-beta-cyclodextrin effected the separation. The affect on selectivity resulting from the addition of an organic modifier to the run buffer was examined. The low background absorbency of the run buffer made for easy detection of compounds that absorbed at low UV wavelengths. The quick analysis time made this suitable for analysis of combinatorial chemistry samples. 相似文献
4.
Shi Nian-Qing Prahl Kristine Hendrick Jim Cruz Jose Lu Ping Cho Jae-Yong Jones Sharon Jeffries Thomas 《Applied biochemistry and biotechnology》2000,84(1-9):201-216
Pichia stipitis CBS 6054 will grow on d-xylose, d-arabinose, and l-arabinose. d-Xylose and l-arabinose are abundant in seed hulls of maize, and their utilization is important in processing grain residues. To elucidate
the degradation pathway for l-arabinose, we obtained a mutant, FPL-MY30, that was unable to grow on d-xylose and l-arabinose but that could grow on d-arabinitol. Activity assays of oxidoreductase and pentulokinase enzymes involved in d-xylose, d-arabinose, and l-arabinose pathways indicated that FPL-MY30 is deficient in d-xylitol dehydrogenase (D-XDH), d- and l-arabinitol dehydrogenases, and d-ribitol dehydrogenase. Transforming FPL-MY30 with a gene for xylitol dehydrogenase (PsXYL2), which was cloned from CBS 6054 (Gen Bank AF127801), restored the D-XDH activity and the capacity for FPL-MY30 to grow on
l-arabinose. This suggested that FPL-MY30 is critically deficient in XYL2 and that the d-xylose and l-arabinose metabolic pathways have xylitolas a common intermediate. The capacity for FPL-MY30 to grow on d-arabinitol could proceed through d-ribulose. 相似文献
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A. Raith W. T. Perkins N. J. G. Pearce T. E. Jeffries 《Fresenius' Journal of Analytical Chemistry》1996,355(7-8):789-792
Laser ablation inductive coupled plasma – mass spectrometry (LA-ICP-MS) was used to analyse minor and trace elements in the hard parts of a shellfish, representing the environmental changes. A large, long-lived bivalve such as Arctica islandica can produce historical records of trace element fluctuations in seawater. Analytical traverses using LA-ICP-MS were performed from the inner to the outer wall of the shell at different locations. The development of the UV laser system now offers spatial resolution of craters of 10 m diameter, enabling several analyses between the various growthbands. The elemental changes between growthbands of the shell are discussed, showing the potential for precise determination of heavy-metal pollution over the years. 相似文献
7.
A. Raith W. T. Perkins N. J. G. Pearce T. E. Jeffries 《Analytical and bioanalytical chemistry》1996,355(7-8):789-792
Laser ablation inductive coupled plasma – mass spectrometry (LA-ICP-MS) was used to analyse minor and trace elements in the hard parts of a shellfish, representing the environmental changes. A large, long-lived bivalve such as Arctica islandica can produce historical records of trace element fluctuations in seawater. Analytical traverses using LA-ICP-MS were performed from the inner to the outer wall of the shell at different locations. The development of the UV laser system now offers spatial resolution of craters of 10 μm diameter, enabling several analyses between the various growthbands. The elemental changes between growthbands of the shell are discussed, showing the potential for precise determination of heavy-metal pollution over the years. 相似文献
8.
A mutant strain ofPichia stipitis, FPL-061, was obtained by selecting for growth on L-xylose in the presence of respiratory inhibitors. The specific fermentation
rate of FPL-061, was higher than that of the parent,Pichia stipitis CBS 6054, because of its lower cell yield and growth rate and higher specific substrate uptake rate. With a mixture of glucose
and xylose, the mutant strain FPL-061 produced 29.4 g ethanol/L with a yield of 0.42 g ethanol/g sugar consumed. By comparison,
CBS 6054 produced 25.7 g ethanol/L with a yield of 0.35 gJg. The fermentation was most efficient at an aeration rate of 9.2
mmoles O2 L-1 h-1. At high aeration rates (22 mmoles O2 L-1 h-1), the mutant cell yield was less than that of the parent. At low aeration rates, (1.1 to 2.5 O2 L-1 h-1), cell yields were similar, the ethanol formation rates were low, and xylitol accumulation was observed in both the strains.
Both strains respired the ethanol once sugar was exhausted. We infer from the results that the mutant, P.stipitis FPL-061, diverts a larger fraction of its metabolic energy from cell growth into ethanol production. 相似文献
9.
A. D. McCartt S. Gates P. Lappas J. B. Jeffries R. K. Hanson 《Applied physics. B, Lasers and optics》2012,106(3):769-774
The interaction of endospore-laden bioaerosols and shock waves is monitored with a combination of laser absorption and scattering.
Tests are performed in the Stanford aerosol shock tube for post-shock temperatures ranging from 400–1100 K. In situ laser
measurements at 266 and 665 nm provide a real-time monitor of endospore morphology. Scatter of visible light measures the
integrity of endospore structure, while absorption of UV light provides a monitor of biochemicals released by endospore rupture.
For post-shock temperatures greater than 750 K endospore morphological breakdown is observed. A simple theoretical model is
employed to quantify the optical measurements, and mechanisms leading to the observed data are discussed. 相似文献
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