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Streubel R Schiemann U Jones PG Tran Huy NH Mathey F 《Angewandte Chemie (International ed. in English)》2000,39(20):3686-3688
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Cruz-Aguado JA Chen Y Zhang Z Elowe NH Brook MA Brennan JD 《Journal of the American Chemical Society》2004,126(22):6878-6879
Firefly luciferase (FL) was entrapped in sol-gel-derived silica containing precursors based on covalent linkage of d-gluconolactone or d-maltonolactone to (aminopropyl)triethoxysilane to form N-(3-triethoxysilylpropyl)gluconamide or N-(3-triethoxysilylpropyl)maltonamide. The enzyme was active and stable in this material and showed catalytic constants close to those in solution. As little as 20 amol ATP could be detected with the entrapped FL, and the entrapped enzyme could be used over several cycles. 相似文献
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The lipopolysaccharide (LPS)-rich outer membrane of gram-negative bacteria provides a protective barrier that insulates these organisms from the action of numerous antibiotics. Breach of the LPS layer can therefore provide access to the cell interior to otherwise impermeant toxic molecules and can expose vulnerable binding sites for immune system components such as complement. Inhibition of LPS biosynthesis, leading to a truncated LPS molecule, is an alternative strategy for antibacterial drug development in which this vital cellular structure is weakened. A significant challenge for in vitro screens of small molecules for inhibition of LPS biosynthesis is the difficulty in accessing the complex carbohydrate substrates. We have optimized an assay of the enzymes required for LPS heptose biosynthesis that simultaneously surveys five enzyme activities by using commercially available substrates and report its use in a small-molecule screen that identifies an inhibitor of heptose synthesis. 相似文献
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Background
Genetically manipulated embryonic stem (ES) cell derived neurons (ESNs) provide a powerful system with which to study the consequences of gene manipulation in mature, synaptically connected neurons in vitro. Here we report a study of focal adhesion kinase (FAK), which has been implicated in synapse formation and regulation of ion channels, using the ESN system to circumvent the embryonic lethality of homozygous FAK mutant mice. 相似文献8.
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MR Eskildsen AB Abrahamsen PL Gammel DJ Bishop NH Andersen K Mortensen PC Canfield 《Pramana》2002,58(5-6):903-905
We compare the results of small angle neutron scattering on the flux line lattice (FLL) obtained in the borocarbide superconductor
LuNi2B2C with the applied field along the c- and a-axes. For H‖c the temperature dependence of the FLL structural phase transition from square to hexagonal symmetry was investigated. Above
10 K the transition onset field. H
2(T), rises sharply, bending away from H
c2(T) in contradiction to theoretical predictions of the two merging. For H‖a a first order FLL reorientation transition is observed at H
tr=3–3.5 kOe. Below H
tr the FLL nearest neighbor direction is parallel to the b-axis, and above H
tr to the c-axis. This transition cannot be explained using nonlocal corrections to the London model. 相似文献
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