全文获取类型
收费全文 | 602篇 |
免费 | 17篇 |
国内免费 | 2篇 |
专业分类
化学 | 527篇 |
晶体学 | 1篇 |
力学 | 7篇 |
数学 | 9篇 |
物理学 | 77篇 |
出版年
2023年 | 2篇 |
2022年 | 4篇 |
2021年 | 7篇 |
2020年 | 3篇 |
2019年 | 14篇 |
2018年 | 6篇 |
2017年 | 4篇 |
2016年 | 6篇 |
2015年 | 6篇 |
2014年 | 12篇 |
2013年 | 25篇 |
2012年 | 33篇 |
2011年 | 34篇 |
2010年 | 17篇 |
2009年 | 17篇 |
2008年 | 29篇 |
2007年 | 32篇 |
2006年 | 35篇 |
2005年 | 29篇 |
2004年 | 30篇 |
2003年 | 29篇 |
2002年 | 32篇 |
2001年 | 10篇 |
2000年 | 18篇 |
1999年 | 12篇 |
1997年 | 5篇 |
1996年 | 7篇 |
1995年 | 9篇 |
1994年 | 7篇 |
1993年 | 4篇 |
1992年 | 9篇 |
1991年 | 6篇 |
1990年 | 8篇 |
1989年 | 7篇 |
1988年 | 5篇 |
1987年 | 9篇 |
1986年 | 10篇 |
1985年 | 8篇 |
1984年 | 13篇 |
1983年 | 4篇 |
1982年 | 6篇 |
1981年 | 5篇 |
1980年 | 6篇 |
1979年 | 11篇 |
1978年 | 6篇 |
1977年 | 6篇 |
1976年 | 8篇 |
1975年 | 4篇 |
1974年 | 6篇 |
1972年 | 3篇 |
排序方式: 共有621条查询结果,搜索用时 19 毫秒
1.
Simultaneous quantification of leukotrienes and hydroxyeicosatetraenoic acids in cell culture medium using liquid chromatography/tandem mass spectrometry 下载免费PDF全文
Leukotrienes (LTs) and hydroxyeicosatetraenoic acids (HETEs) are important bioactive lipid mediators that participate in various pathophysiological processes. To advance understanding of the mechanisms that regulate these mediators in physiological and pathological processes, an analytical method using liquid chromatography/tandem mass spectrometry for the simultaneous quantification of LTB4, LTC4, LTD4, LTE4, 5‐HETE, 8‐HETE, 12‐HETE and 15‐HETE in cell culture media was developed. A Supel?‐Select HLB solid‐phase extraction cartridge was used for sample preparation. The compounds were separated on a C18 column using gradient elution with acetonitrile–water–formic acid (20:80:0.1, v/v/v) and acetonitrile–formic acid (100:0.1, v/v). The calibration curves of LTB4, LTD4, LTE4 and HETEs were linear in the range of 0.025–10 ng/mL, and the calibration curve of LTC4 was linear in the range of 0.25–10 ng/mL. Validation assessment showed that the method was highly reliable with good accuracy and precision. The stability of LTs and HETEs was also investigated. Using the developed method, we measured LTs and HETEs in the culture supernatant of the human mast cell line HMC‐1. The present method could facilitate investigations of the mechanisms that regulate the production, release and signaling of LTs and HETEs. Copyright © 2014 John Wiley & Sons, Ltd. 相似文献
2.
Masashi Ohkawa Yuki Shirai Takeshi Goto Seishi Sekine Takashi Sato 《Fiber and Integrated Optics》2002,21(2):105-113
In this paper, a silicon-based integrated optic pressure sensor using an intermodal interference between the fundamental TM-like and TE-like modes is described. The sensor consists of a micromachined rectangular diaphragm and a straight polystyrene optical waveguide passing over the diaphragm. Its sensitivity is theoretically known to be strongly dependent on the position of the waveguide over the diaphragm. To experimentally investigate such dependence, we fabricated a sensor with a 1.2 mm ×10 mm ×20 μm diaphragm, over which waveguides were placed at 50 μm intervals. The measured phase sensitivity was 98 mrad/kPa for the waveguide nearest to the diaphragm edge. The measurement was also carried out for the other waveguides. As theoretically expected, the largest sensitivity was obtained for the waveguide nearest to the edge. 相似文献
3.
T Utsunomiya M. Sekine 《International Journal of Infrared and Millimeter Waves》2005,26(11):1651-1660
We have conducted a millimeter wave propagation experiment at 103 GHz (2.9 mm) on a propagation path of 390 m. The results
were compared with the rain attenuation calculations from the Marshall-Palmer, Best, Joss-Thomas-Waldvogel and Weibull distributions
for raindrop-size. It has been shown that the Weibull distribution has a good agreement with the experiments. 相似文献
4.
The rain attenuation was calculated by using the Marshall-Palmer, Best, Joss-Thomas-Waldvogel and Weibull distributions for raindrop-size. The results were compared with the recent measurements from 8 to 312.5 GHz at the rain rate R = 50mm/hr. Especially, the Weibull distribution has a good agreement with the measurements at 312.5 GHz (0.96 mm) in the submillimeter wavelength. Specific attenuation values from 1 to 1000 GHz were calculated for a rain temperature of –10°C, 0°C and 20°C by using the Weibull distribution. 相似文献
5.
Ryoko Chiba Ayako Ogasawara Teppei Kubo Hiroyuki Yamazaki Masuo Umino Yoichi Ishizuka 《Analytical sciences》2003,19(5):785-789
A column-switching high-performance liquid chromatographic method with fluorescence detection was developed for the simultaneous determination of four benzamide-type anti-psychotic drugs: sulpiride, tiapride, sultopride and metoclopramide in human serum. In this method, a TSKgel Super-ODS column was used as an analytical column, and a TSKgel G 2000SW was prepared as a pretreatment column. Under the optimized analytical conditions, four benzamide-type anti-psychotic drugs were eluted within 18 min. The detection limits (S/N = 3) for sulpiride, tiapride, sultopride and metoclopramide are 1 ng/ml, 4 ng/ml, 2 ng/ml and 0.5 ng/ml, respectively. Finally, the method was applied to the determination of sulpiride in human serum samples obtained after a single oral dose of sulpiride. 相似文献
6.
7.
Jun Sekine 《Applied Mathematics and Optimization》2006,54(2):131-158
The dual optimization problem for the exponential hedging problem is addressed with a cone constraint. Without boundedness
conditions on the terminal payoff and the drift of the Ito-type controlled process, the backward stochastic differential equation,
which has a quadratic growth term in the drift, is derived as a necessary and sufficient condition for optimality via a variational
method and dynamic programming. Further, solvable situations are given, in which the value and the optimizer are expressed
in closed forms with the help of the Clark-Haussmann-Ocone formula. 相似文献
8.
Tomomi Kawasaki Miyuki Nagaoka Tomoko Satoh Ayako Okamoto Rie Ukon Atsuyo Ogawa 《Tetrahedron》2004,60(15):3493-3503
Claisen rearrangement triggered by enolization of 2-allyloxyindolin-3-ones with DBU was performed in order to prepare 3-allyl-3-hydroxyindolin-2-ones. Total synthesis of 3-hydroxyindolin-2-one alkaloids, (±)-donaxaridine, as well as (±)-convolutamydines A and E, was achieved by transformation of the allyl moiety of 3-allyl-3-hydroxyindolin-2-ones. 相似文献
9.
Yasufumi Otsubo Masahiro Sekine Shingo Katayama 《Journal of colloid and interface science》1992,150(2)
Monodisperse silica particles were formed by hydrolyzing tetraethylorthosilicate in an ethanol solution and the silica suspensions dispersed in a silicone oil were prepared by a different procedure. The effects of adsorbed water on the electrorheological (ER) behavior were studied under oscillatory shear. The amounts of adsorbed water and surface silanol groups were determined by thermogravimetric analysis. The magnitude of the complex viscosity, |η*|, increases with the application of electric fields. The physically adsorbed water is primarily responsible for the ER effect. However, the fluids containing large amounts of adsorbed water do not always show excellent ER performance. The surface silanol groups have an important role in promoting the ER effect. Not only the amount but also the situation of silanol groups determines the ER activity of adsorbed water. 相似文献