Flow and Sequential Injection Manifolds for the Spectrophotometric Determination of Captopril Based on its Oxidation by Fe(III)

 Two new simple and rapid methods are reported for the accurate and precise spectrophotometric determination of captopril (CPL) using flow (FI) and sequential injection (SI) analysis. The methods are based on the fast oxidation of CPL by Fe(III). The produced Fe(II) reacts with 2,2′-dipyridyl-2-pyridylhydrazone (DPPH) in acidic medium to form a colored complex which is monitored spectrophotometrically at 535 nm. Both methods allow the determination of the analyte up to 1000 mg L⁻¹ at a sampling rate of 120 and 60 injections per hour for FI and SI, respectively. The methods are very precise [s _r=0.8 and 1.2% at 500 mg L⁻¹ CPL (n=12) for FI and SI, respectively] and the 3σ detection limits (c _L=4.0 and 7.0 mg L¹, respectively) are quite satisfactory. Their application to a variety of anti-hypertensive commercial pharmaceutical formulations showed excellent results (relative errors, e _r, < ± 1.6% in all cases compared to an official HPLC method), while common pharmaceutical excipients were found not to interfere. Recovery experiments further verified the accuracy of the developed methods, as the percent recoveries were in the range of 98.1–102.5%. Author for correspondence. E-mail: themelis@chem.auth.gr Received May 9, 2002; accepted January 8, 2003 Published online May 5, 2003     

Liquid chromatographic retention behaviour and separation of promethazine and isopromethazine on a β-cyclodextrin bonded-phase column

A liquid chromatographic method for the separation of promethazine (PR) and its positional isomer isopromethazine (IPR) is described.PR is an N-substituted phenothiazine with the actions and the uses of the antihistamines (H1-receptor antagonists). IPR is an impurity in the pharmaceutical preparations of PR and must be controlled at a level below 1%. The liquid chromatographic behaviour of PR and IPR on a hydrolytically stable β-cyclodextrin (β-CD) column with respect to mobile phase composition, pH, ionic strength and the nature of the organic modifier was also investigated. Based on the results, conditions were chosen for the isocratic separation of the two isomers. The proposed separation method is simple and rapid and permits the simultaneous determination of PR and IPR. The separation selectivity of a cyclodextrin bonded-phase column was examined. Special attention was devoted to modelling the inclusion complexes of PR and IPR with β-CD in order to predict their optimum orientation within the β-CD cavity.     

Etude des dimensions moleculaires des polymeres dissous dans des melanges binaires de solvants polaires et non polaires—I. Polyvinyl-2 pyridine

We have studied the molecular dimensions of poly-2-vinylpyridine in solution in binary solvent mixtures consisting of a non polar and a polar component, viz. benzene-ethanol and benzene-chloroform. We have also studied the preferential solvation of the same polymer in the above mixtures using light scattering. We have observed a conformational transition of P2VP taking place in a composition region for each solvent mixture. This transition shows as a discontinuity in the unperturbed dimensions, in the long range interactions parameter and in the parameter of preferential solvation of the polymer. We think that this transition is related to the existence of two ordered structures of the polymer chain, one stable before and the other after the transition region.     

Universal behavior of flexible and wormlike polymers using relations derived from the blob theory and the statistical mechanical theory

The variation of the viscometric expansion factor of macromolecular chains, α_η, as a function of the number of blobs of which these chains consist, N/N_c, obeys a universal law, both for wormlike and for flexible polymers. Using also wormlike and flexible polymers, we show that the factor relating the excluded volume parameter, z, to the static expansion factor, α_G, varies with the number of statistical chain segments, in a universal manner. The universalities appear to be valid below chain lengths required for the appearance of a power law, and they do not seem to have an upper limit in molecular weight. For these two universalities concerning flexible and wormlike polymers we use relations derived from the blob theory [Polymer 20 (1979) 1083] and statistical mechanical theory [J. Chem. Phys. 57 (1972) 2843].     

Novel disposable bismuth-sputtered electrodes for the determination of trace metals by stripping voltammetry

This work describes a novel type of bismuth electrode for stripping voltammetry based on coating a silicon substrate with a thin bismuth film by means of sputtering. The bismuth-based sensors were characterized by optical methods (scanning electron microscopy (SEM), atomic force microscopy (AFM) and X-ray diffraction (XRD)) and as well as by linear sweep voltammetry. Subsequently, the electrodes were tested for the detection of low concentrations of trace metals (Cd(II), Pb(II) and Ni(II)) by stripping voltammetry. Well-formed stripping peaks were observed for trace concentrations of the target analytes demonstrating “proof-of-principle” for these sensors. This type of electrochemical device, utilizing thin-film technology for the formation of the bismuth film, holds promise for future applications in trace metal analysis.     

A new approach to the determination of the statistical segment length of wormlike polymers

The Stockmayer-Fixman-Burchard (SFB) and the Dondos-Benoit (DB) equations have been applied to determine the unperturbed dimensions parameterK of wormlike polymers. An empirical relation between the Flory's constant and the Mark-Houwink-Sakurada (MHS) exponenta has been proposed. The values found by this equation are lower than the value 2.5×10²³ used in the case of flexible polymers and this deviation is attributed to the influence of the draining effect. From theK value and the so calculated value of , we calculate the Kuhn statistical segment length of wormlike polymers. The obtained — for a great number of wormlike polymers — statistical segment lengths are almost the same as these calculated by the Yamakawa-Fujii and the Bohdanecky methods. The molecular mass regions in which the SFB, the DB, and the MHS equations are valid are explored. A criterion for the distinction between flexible and wormlike polymers is proposed based on the way of approach to the power law.     

QUANTITATION OF PHOTOSYSTEM II ACTIVITY IN SPINACH CHLOROPLASTS. EFFECT OF ARTIFICIAL QUINONE ACCEPTORS

Quantitation of photosystem II (PSII) activity in spinach chloroplasts is presented. Rates of PSII electron-transport were estimated from the concentration of PSII reaction-centers (Chl/PSII = 380:1 when measured spectrophotometrically in the ultraviolet [ΔA₃₂₀] and green [ΔA_540–550] regions of the spectrum) and from the rate of light utilization by PSII under limiting excitation conditions. Rates of PSII electron-transport were measured under the same light-limiting conditions using 2,5-dimethylbenzoquinone or 2,5-dichlorobenzoquinone as the PSII artificial electron acceptors. Evaluation is presented on the limitations imposed in the measurement of PSII electron flow to artificial quinones in chloroplasts. Limitations include the static quenching of excitation energy in the pigment bed by added quinones, the fraction of PSII centers (PSII_β) with low affinity to native and added quinones, and the loss of reducing equivalents to molecular oxygen. Such artifacts lowered the yield of steady-state electron transport in isolated chloroplasts and caused underestimation of PSII electron-transport capacity. The limitations described could explain the low PSII concentration estimates in higher plant chloroplasts (Chl/PSII = 600 ± 50) resulting from proton flash yield and/or oxygen flash-yield measurements. It is implied that quantitation of PSII by repetitive flash-yield methods requires assessment of the slow turnover of electrons by PSII_β and, in the presence of added quinones, assessment of the PSII quantum yield.     

Molecular dynamics simulations of gas diffusion in metal-organic frameworks: argon in CuBTC

The class of coordination polymers known as metal-organic frameworks (MOFs) has three-dimensional porous structures that are considered as a promising alternative to zeolites and other nanoporous materials for catalysis, gas adsorption, and gas separation applications. In this paper, we present the first study of gas diffusion inside an MOF and compare the observed diffusion to known behaviors in zeolites. Using grand canonical Monte Carlo and equilibrium molecular dynamics, we calculate the adsorption isotherm and self-, corrected, and transport diffusivities for argon in the CuBTC metal-organic framework. Our results indicate that diffusion of Ar in CuBTC is very similar to Ar diffusion in silica zeolites in magnitude, concentration, and temperature dependence. This conclusion appears to apply to a broad range of MOF structures.     

Investigation for the determination of ultra trace amounts of Co2+ complexed with 1,10-phenanthroline by adsorptive voltammetry

Summary The spontaneous adsorption of the Co²⁺ complex with 1,10-phenanthroline on HMDE was utilized for the development of a voltammetric analytical method employing phase selective alternating current, with first harmonic AC₁ in phase, =0°, voltammetry. The dependence of the analytical voltammetric response on physical and chemical parameters in various supporting electrolytes was investigated in detail. Optimum conditions for Co²⁺ determination were established. The method is very sensitive and selective. The determination limit is 205 ng/l Co²⁺ (adsorption time, t_ads=20 min). The precision and accuracy were evaluated: 1.84 g/l Co²⁺, RSD=3.24%, RE=–0.54%; for 205 ng/l Co²⁺, RSD=4.85%, RE=–3.90%. The results showed good reproducibility and reliabiality. The method was applied to natural waters, wines, mussel tissue and aquatic algae certified reference material.