野生大豆(Glycine soja SH1)球蛋白glycinin Gy4基因家族的两种表达拷贝

本文研究我国野生大豆(Glycine soja)种子贮藏蛋白基因的结构,并与栽培大豆进行比较,构建成野生大豆Glycine soja SH1和栽培大豆Glycine max子叶cDNA库。用已知含球蛋白glycinin Gy 4基因的克隆DNA λS 312为探针,从两个cDNA库中分离出6个克隆。其中两个克隆pWS 228与pWS 242含全长cDNA,克隆cDNA的限制酶图谱和cDNA与基因组DNA的Southern法杂交表明它们代表野生大豆球蛋白glycinin Gy 4基因家族的两种表达拷贝。pWS 228 cDNA的部分序列已测定并与栽培大豆相应顺序作比较。分子杂交还证明Ⅱ类glycinin基因家族的组编在野生大豆胚形成过程中的变动。     

TWO EXPRESSED COPIES OF GLYCININ Gy4 GENE SUBFAMILY IN WILD SOYBEAN Glycine soja SHI

To study the structure of genes encoding seed storage proteins of the wild variety of soybean (Glycine soja) in comparison with those of the cultivated (Glycine max),cDNA libraries from cotyledon poly(A)~+ RNA of both wild and cultivated soybeans were constructed.Six clones were isolated using the genomic clone λS312 which contains the coding sequence for the glycinin subunit precursor (A_5A_4B_3) as probe for molecular hybridization. Two clones pWS228 and pWS242 contain the full length of cDNA.Restriction mapping of the cloned cDNA and Southern hybridization of the cDNA with genomic DNA showed two expressed copies of glycinin Gy4 gene subfamily in wild soybean Glycine soja SHI. Alteration of DNA arrangement in group-Ⅱ glycinin gene subfamily during embryogenesis was also demonstrated.     

大豆(Glycine max)贮藏蛋白两种编码顺序的克隆与性质

本文证明大豆(Glycine max)贮藏蛋白的两种编码顺序已经克隆。并通过分子杂交和体外翻译研究了它们的性质。其中一顺序与两种分子量不同的mRNA杂交,杂交选择mRNA在体外翻译成60kd和42kd多肽,推测为7S贮藏蛋白的α和β亚基,另一顺序则与分子量为0.7×10~6dalton的mRNA杂交,杂交选择mRNA在体外翻译成57kd和49kd多肽。     

CLONING AND CHARACTERIZATION OF TWO CODING SEQUENCES FOR STORAGE PROTEIN IN SOYBEAN Glycine max

Two coding sequences for storage protein in Glycine max have been cloned. They were characterized by molecular hybridization and in vitro translation. One sequence happens to hybridize to two sized mRNAs which are translated in vitro to 60 Kd and 42 Kd polypeptides corresponding to a and β subunits of 7S storage protein, and the other is hybridizable with 0.7×10~6 dalton mRNA giving rise to 57 Kd and 49 Kd polypeptides in vitro translation.