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The performance of small-bore columns of 1 mm I. D. for HPLC is described. The effect of mobile phase velocity and connecting effect on the values of H, a′, b′ and β are investigated. By operating at mobile phase velocity of 0.8 mm/s, the column of 200×1 mm I. D. packed with YWG-5μm (silica gel) can give the efficiency of about 11,000 plates which meet the needs of ordinary analysis. Oompared with column of 150×2 mm I. D., the solvent consumption is reduced to 1/3. The value of n~∞/t_r~0 about 700 T. P./s can be obtained. But the value of β is rather large. It needs further to be improved. Coupling 5 columns of 200×1 mm I. D., the efficiency of about 50,000 plates can be obtained. For a column of 1000×1 mm I. D. packed directly, the efficiency of 70,000 plates was achieved. Some applications of 1 mm I. D. column are given. We propose to use the column of 200×1mm I. D. in the routine analysis. 相似文献
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本文采用自制的专门设计的柱系统可使“边壁效应”减至最小.不同溶质在不同内径的柱子上其保留体积VR和峰宽(体积单位)间有一近似的线性关系,2△V1/2=a’+b’VR. 文中系统考查了鉴定器系统体积对a’和b’值的影响,指出鉴定器系统的体积对a’值有很大的影响,而对b’值几乎没有什么影响.使用改进的柱系统和自制的紫外鉴定器.在2毫米内径10厘米长的柱子上获得了6000理论塔板数. 相似文献
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本文报道了内径为1mm的高效液相色谱柱性能,考察了冲洗剂线速度和接头效应对理论塔板高度H和a′,b′,β′值的影响.当流动相线速为 0.8mm/s时,φ1×200mm柱柱数可达11000理论塔板以上,已能满足大多数常规分析的需要.其溶剂消耗量仅为柱效相近的φ2×150mm 柱的 1/3,n~∞/t_r~o值可达700 T.P/s,但此时β较大,有待进一步改进.5根20 cm长柱串联后柱效可达50000 T.P,直接装填的1m 柱柱效可达70000 T.P.文中给出了快速分离谱图和复杂样品的分离谱图.建议在日常分析中采用φ1×200mm柱。 相似文献
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