排序方式: 共有91条查询结果,搜索用时 182 毫秒
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We measured the fluorescence spectra of the whole blood, the red blood cell (RBC) and the hemoglobin using 457.9-nm Ar+ laser excitation. It was found that the fluorescence spectra of the whole blood and the RBC have much similarities in the intensity, the emission peaks and the emitting region, and abundant peaks can be found. But for the hemoglobin, fluorescence could only be found in the wavelength range 580-650 nm. It was concluded that in the wavelength range of 650-850 nm, the fluorescence spectra were emitted by the new fluorophores generated by the breakdown of some weak bonds on the RBC membrane,such as the C-C bond and the C-N bond. In the wavelength range of 590 - 650 nm, the fluorescence spectra are mainly emitted by the hemoglobin, but the hemoglobin solution of cracked RBC has a strong quencher effect on the fluorescence spectrum. The experimental result and the theoretical analysis are meaningful for the medical diagnostics and the therapy. 相似文献
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研究了紫外光照射乙醇-水混合溶液的稳态和时间分辨荧光光谱.通过检测其荧光光谱和激发光谱,得到了稳态发射光谱的三个荧光峰,峰值分别位于290 nm、305 nm、330 nm,相应的最佳激励光分别为265 nm, 280 nm 和236 nm.在荧光光谱峰值波长处分别监测其荧光强度随时间的衰变过程,将获得的荧光衰减动力学曲线采用指数拟合并进行解卷积处理获得不同荧光光子的寿命值.乙醇-水溶液稳态光谱的特点和三个不同的荧光寿命都表明了溶液中含有三个不同的生色团,分析认为乙醇和水分子间通过氢键作用形成了不同结构的团簇分子. 相似文献
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A numerical model is developed for the calculation of transient temperature field of thin film coating induced by a long-pulsed high power laser beam. The electric field intensity distribution of HfO2/Si02 high reflective (HR) film is investigated to calculate the thermal field of the film. The thermal-mechanical relationships are discussed to predict the laser damage area of optical thin film under long pulse high energy laser irradiation. 相似文献
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A theoretical model is proposed to describe the mechanism of
laser-induced plasma shock wave evolution in air. To verify the
validity of the theoretical model, an optical beam deflection technique
is employed to track the plasma shock wave evolution process. The
theoretical model and the experimental signals are found to be in
good agreement with each other. It is shown that the laser-induced
plasma shock wave undergoes formation, increase and decay processes;
the increase and the decay processes of the laser-induced plasma
shock wave result from the overlapping of the compression wave and
the rarefaction wave, respectively. In addition, the laser-induced
plasma shock wave speed and pressure distributions, both a
function of distance, are presented. 相似文献
89.
乙醇-水溶液中团簇分子的基元荧光光谱研究 总被引:2,自引:0,他引:2
采用高斯分解法对236 nm的紫外光激发乙醇体积浓度为10%~95%的乙醇-水二元混合物产生的荧光光谱进行分解,得到了荧光谱线里面所包含的基元峰,发现每种浓度溶液的谱线都是由8个基元高斯峰组成的,并获得了每个基元谱线的相对强度、峰值位置数据。对这些高斯峰及相应参数进行分析,并结合乙醇-水混合物的荧光发射机理得到了各种发光团簇的相对大小以及构象信息,进一步计算了它们各自的荧光主发射跃迁能。另外,还对高斯峰半高宽与溶液中分子间缔合形态之间的联系做了初步探讨。研究结果可为进一步研究乙醇-水二元混合物的微观结构和特性提供理论和实验依据。 相似文献
90.
人血清血卟啉荧光光谱的双正交样条小波识别 总被引:2,自引:0,他引:2
人血清中血卟啉含量很低,其荧光光谱强度很弱,往往呈肩峰形态,难以识别其位置和荧光强度。双正交样条小波可实现对其弱信号的分辨。在血清背景荧光光谱上叠加血卟啉荧光信号,并改变其荧光强度,得到一系列强度改变的 血清血卟啉荧光光谱。用双正交样条小波bior5.5对获取的荧光光谱通过7次小波分解,将背景与卟啉荧光信号分离,得到了离散逼近信号(a1~a7)和离散细节信号(d1~d7)。结果 表明,信号频率随着分解次数的增加逐步降低。当分解到第7次时,出现了血卟啉荧光特征峰。信号峰的位置随着信号强度的下降蓝移约2.5 nm,而通过小波滤波器提取的信号峰的位置不变。随着信号的强度降低,信号峰的位置逐渐消失 ,因而其荧光强度与荧光峰的位置无法确定,而通过小波滤波器提取的信号则不受影响,且灵敏度高,从而实现了用双正交样条小波对人血清血卟啉荧光光谱的识别。由于小波变换是线性变换,离散细节信号保持信号原有的线性关系,可 以用来对血清中所含血卟啉的准确成份和定量信息,从而进行定性和定量分析,为血清荧光光谱用于肿瘤的早期诊断提供了一种方法。 相似文献