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81.
We propose a mathematical model to analyse the membrane tether formation process on a cell surface with reservoir. Based on the experimental results, the membrane reservoir density of breast cancer cell was obtained, ρ=8.02. The membrane surface viscosity between membrane and environment η is 0.021(pN.s/μm^3), and the static force F0=5.71pN. 相似文献
82.
基于标准的Hanbury-Brown-Twiss探测方式下,研究了具有Poisson统计背景的实际单分子光子源与理想双分子系统的光子统计特性,通过比较分析二者在具有相同平均光子数n—时的Mandel参数,研究给出一种利用双光子的统计概率P(2)判别单分子的方法为P(2)<12(2-4-2n—)2.同时研究了单分子光子源的信号/背景比对分析结果的影响.
关键词:
单分子
Mandel参数
Poisson分布
信号/背景比 相似文献
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An efficient scheme for photon statistics measurement is presented based on the Hanbury-Brown-Twiss configuration. We set the sampling time Ts to satisfy the relationship of Ts 〈 Td 〈 Tm, where Td is the dead time of each detector and Tm is the laser pulse repetition period. And each single photon detector cannot detect more than one photon in each pulse. The approach can sufficiently eliminate the influences of the detector's dead time on photon statistics. At last, the photon statistics of coherent field is experimentally determined. 相似文献
86.
Appropriate Gate Time for Single Molecular Photon Detection Based on Optimal Signal-to-Noise Ratio Analyses 下载免费PDF全文
Signal-to-noise ratio of fluorescence detection from a single molecule & analysed by using time-gated techniques. It is found that the optimal signal-to-noise ratio can be obtained by choosing an appropriate gate time with a certain optical background. The dependences of molecular fluorescence lifetime and the optimal signal-to-noise ratio on the appropriate gate time are respectively discussed with two kinds of background sources~ chaotic state with uniform distribution and coherent state with exponential distribution in time domain. For chaotic state background we find that a certain range for appropriate gate time can be obtained with a definite fluorescence lifetime, larger fluorescence lifetime would lower the value of optimal signal-to-noise ratios. For coherent state background we find that there is also a narrow range of appropriate gate time when lifetime of single molecule is less than that of background photons. 相似文献
87.
An ultrahigh resolution photoassociation spectrum of caesium atoms in a magneto-optical trap is presented. Hyperfine structure of the excited state molecule is obtained by using the lock-in method based on modulated cold atoms in this spectrum. Amplitude of resonant lines related to the rotational levels increases with photoassociation laser intensity, and saturation effect of photoassociation of cold atoms is observed in our experiment. The saturation intensity of photoassociation is deduced by fitting the experimental data to asaturation model based on scattering theory. Differences among saturation intensities of different rotational progressions in the υ=55 vibrational state of the caesium molecular long-range 0g- state have been found. 相似文献
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通过在不同信号背景比下的最佳信号噪声比分析,讨论单分子光子源背景信号分别为时间均匀分布和指数特性分布时同步取样时间的选取.在Hanbury-Brown-Twiss探测方式下,测量光子源的单事件光子统计概率P(n,n=0、1、2).研究给出在P(1)2P(2)-3P(2)时,直接测量单分子光子源的信号背景比的方法SBR=[P(1)]2/2P(2).当信号背景比SBR>2.41时研究给出判别单分子的方法P(2)<(2-4-2n-)2/2,n为每个激发周期内探测到的平均光子数. 相似文献
90.