散射光场中的光学涡旋

散射光场中的光学涡旋叶茂，王鹏业，戴建华，张洪钧（四川大学物理系成都６１００６４）（中国科学院物理研究所北京１０００８０）ＯｐｔｉｃａｌＶｏｒｔｉｃｅｓｉｎＡＴｈｅｒｍａｌＲａｙｌｅｉｇｈＳｃａｔｔｅｒｉｎｇＬｉｇｈｔＦｉｅｌｄ￥ＭａｏＹｅ；ｐｅｎｇ...     

原子力显微镜观测生物大分子图像的一种处理方法

针对具有复杂结构的生物大分子图像,本文改进了已有的半自动化方法,设计了一种新的算法去消除背景噪声,目的在于能够从生物大分子图像高效准确地提取量化信息.通过利用这种新的处理程序对生物大分子DNA原子力显微镜图像进行了研究,不仅可以对线性构像的DNA图像进行了处理,而且对于具有分支构像复杂DNA图像也能够处理.本文所提出的数字图像处理方法具有普适性,它也可以被应用到其他成像领域. 关键词： 原子力显微镜 数字图像处理 DNA     

用单分子磁镊研究顺铂导致的DNA凝聚

本文报道了用单分子磁镊研究抗癌药顺铂导致的DNA凝聚过程.结果表明,当拉力比较小时,DNA凝聚的长度-时间曲线是连续缩短和阶跃缩短并存的复杂曲线.而当拉力较大但又不足以阻止DNA的凝聚时,凝聚曲线为连续缩短的双曲线.增加顺铂浓度只会增大凝聚速度而不会改变反应曲线的形状.实验结果与下列成环凝聚模型一致：在水溶液中顺铂能够与DNA形成双臂加合物,也能形成单臂加合物.当顺铂使DNA长链上相距较远的碱基间发生远程交联时,形成小环,导致DNA凝聚.小环间的进一步交联会引起DNA的完全凝聚.DNA凝聚产物十分稳定. 关键词： 顺铂 DNA凝聚 单分子操纵 抗癌药     

Modelling of a DNA packaging motor

During the assembly of many viruses, a powerful molecular motor packages the genome into a preassembled capsid. The Bacillus subtilis phage φ 29 is an excellent model system to investigate the DNA packaging mechanism because of its highly efficient \textitin vitro DNA packaging activity and the development of a single-molecule packaging assay. Here we make use of structural and biochemical experimental data to build a physical model of DNA packaging by the φ 29 DNA packaging motor. Based on the model, various dynamic behaviours such as the packaging rate, pause frequency and slip frequency under different ATP concentrations, ADP concentrations, external loads as well as capsid fillings are studied by using Monte Carlo simulation. Good agreement is obtained between the simulated and available experimental results. Moreover, we make testable predictions that should guide future experiments related to motor function.     

Cytoplasmic Ca2＋ Dynamics under the Interplay between the Different IP3R Gating Models and the Plasma Membrane Ca2＋ Influx

The cytoplasmic Ca2＋ oscillations are investigated under the effect of CRAC channels in non-excitable cells （especially in T cells）. The oscillatory Ca2＋ signals can be modulated as the amplitude-, frequency- and mixed amplitude-frequency modulation modes dependent on the different IP3R gating models. Bifurcation analyses show that Ca2＋ signals in the single positive feedback loop model is a mixed modulation mode. In contrast, Ca2＋ signals in the Mak-McBride-Foskett model demonstrates approximately the frequency modulation mode only with slight amplitude shifts.     

Spiking Regularity and Coherence in Complex Hodgkin-Huxley Neuron Networks

We study the effects of the strength of coupling between neurons on the spiking regularity and coherence in a complex network with randomly connected Hodgkin-Huxley neurons driven by colored noise. It is found that for the given topology realization and colored noise correlation time, there exists an optimal strength of coupling, at which the spiking regularity of the network reaches the best level, Moreover, when the temporal regularity reaches the best level, the spatial coherence of the system has already increased to a relatively high level. In addition, for the given number of neurons and noise correlation time, the values of average regularity and spatial coherence at the optimal strength of coupling are nearly independent of the topology realization. Furthermore, there exists an optimal value of colored noise correlation time at which the spiking regularity can reach its best level. These results may be helpful for understanding of the real neuron world.     

Brownian dynamics simulation of the cross-talking effect among modified histones on conformations of nucleosomes

Using Brownian dynamics simulation,we studied the effect of histone modifications on conformations of an array of nucleosomes in a segment of chromatin.The simulation demonstrated that the segment of chromatin shows the dynamic behaviour that its conformation can switch between a state with nearly all of the histones being wrapped by DNA and a state with nearly all of the histones being unwrapped by DNA,thus involving the "cross-talking" interactions among the histones.Each state can stay for a sufficiently long time.These conformational states are essential for gene expression or gene silence.The simulation also shows that these conformational states can be inherited by the daughter DNAs during DNA replication,giving a theoretical explanation of the epigenetic phenomenon.     

Electric Field-Induced Fluid Velocity Field Distribution in DNA Solution

We present an analytical solution for fluid velocity field distribution of polyelectrolyte DNA. Both the electric field force and the viscous force in the DNA solution are considered under a suitable boundary condition. The solution of electric potential is analytically obtained by using the linearized Poisson-Boltzmann equation. The fluid velocity along the electric field is dependent on the cylindrical radius and concentration. It is shown that the electric field-induced fluid velocity will be increased with the increasing cylindrical radius, whose distribution also varies with the concentration