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41.
Keiko Doi-Kawano Etsuko Nishimoto Yoshiaki Kouzuma Daisuke Takahashi Shoji Yamashita Makoto Kimura 《Journal of fluorescence》2009,19(4):631-639
The steady-state and time-resolved fluorescece spectroscopy is one of the most powerful method to detect and analyze subtle
conformation change and interaction between peptide elements in protein. Phytocystatin Scb isolated from sunflower seeds includes
a single Trp residue at position 85. In an attempt to investigate the interaction of the N-terminal region of Scb with the
first and second hairpin loops by fluorescence spectroscopy of Trp residue, two Scb mutants in which single Trp locates at
position 52 and 58, respectively, and their N-terminal removed mutants were generated. The N-terminal truncation changed the
fluorescence decay kinetics of Trp52 from the triple exponential to double. Furthermore, the time-resolved fluorescence anisotropy
residue indicated that the segmental motion of Trp52 was significantly enhanced by its N-terminal truncation. In contrast,
Trp58 and Trp85 had little influence. The N-terminal successive truncations of Scb and its mutants resulted in the weaken
inhibitors to papain. These results suggested that the N-terminal region of Scb interacts with the peptide segment preceding
the first hairpin loop, thereby stabilizing the conformation of the hairpin loop structure. 相似文献
42.
If a small transparent particle is illuminated with a short laser pulse, the signals of the individual scattering light orders appear temporally successively. Since to each scattered light order belongs a specific optical path through the particle, the particle size can be determined from the time difference between the detected scattered light signals. For the case of a detector position within the backscatter region, which especially is important in measuring practice, the time difference between the specular reflection signal and the signal after a single internal reflection (refraction of second order) must to be evaluated. In the numerical simulation we generate the concerned scattered light signals by using time-resolved Mie calculations and in this paper we present the geometrical models, which permit a correct interpretation of the temporal behavior of these pulse-induced scattered light signals. 相似文献
43.
Algorithms for separable nonlinear least squares with application to modelling time-resolved spectra
Katharine M. Mullen Mikas Vengris Ivo H. M. van Stokkum 《Journal of Global Optimization》2007,38(2):201-213
The multiexponential analysis problem of fitting kinetic models to time-resolved spectra is often solved using gradient-based
algorithms that treat the spectral parameters as conditionally linear. We make a comparison of the two most-applied such algorithms,
alternating least squares and variable projection. A numerical study examines computational efficiency and linear approximation
standard error estimates. A new derivation of the Fisher information matrix under the full Golub-Pereyra gradient allows a
numerical comparison of parameter precision under variable projection variants. Under the criteria of efficiency, quality
of standard error estimates and parameter precision, we conclude that the Kaufman variable projection technique performs well,
while techniques based on alternating least squares have significant disadvantages for application in the problem domain. 相似文献
44.
45.
Time-ResolvedPhotoluminescenceSpectraofPorousSiFEIHao-sheng,HANLi,CHEYan-long,NIERui-juanandLITie-jin(DeparrmentofPhysicsandD... 相似文献
46.
W. G. J. H. M. van Sark P. L. T. M. Frederix D. J. van den Heuvel A. A. Bol J. N. J. van Lingen C. de Mello Donegá H. C. Gerritsen A. Meijerink 《Journal of fluorescence》2002,12(1):69-76
Room-temperature time-resolved luminescence measurements on single CdSe/ZnS quantum dots (QDs) are presented. Fluorescence emission spectra were recorded over periods of up to 30 minutes with a time resolution as small as 6 ms. For QDs in ambient air, a clear 30–40 nm blue shift in the emission wavelength is observed, before the luminescence stops after about 2–3 minutes because of photobleaching. In a nitrogen atmosphere, the blue shift is absent while photobleaching occurs after much longer times (i.e., 10–15 minutes). These observations are explained by photoinduced oxidation. The CdSe surface is oxidized during illumination in the presence of oxygen. This effectively results in shrinkage of the CdSe core diameter by almost 1 nm and consequently in a blue shift. The faster fading of the luminescence in air suggests that photoinduced oxidation results in the formation of non-radiative recombination centers at the CdSe/CdSeOx interface. In a nitrogen atmosphere, photoinduced oxidation is prevented by the absence of oxygen. Additionally, a higher initial light output for CdSe/ZnS QDs in air is observed. This can be explained by a fast reduction of the lifetime of the long-lived defect states of CdSe QDs by oxygen. 相似文献
47.
The adsorption of 4-cyanopyridine (PyCN) on Ag electrode has been studied by surface-enhanced Raman scattering. The Raman band of the CN group is at 2240 or 2100 cm?1; it appears in various ranges of potential. When a series of potentials was applied stepwise to the electrode, the growing and vanishing of both bands was monitored by an optical multichannel analyzer with a time resolution 1 s or less. Some information about the kinetics of the adsorption and desorption of CN group and about the adsorption geometry of PyCN on the electrode was obtained. 相似文献
48.
49.
《Journal of Macromolecular Science: Physics》2013,52(4-6):1023-1032
Real-time SAXS and WAXS patterns have been simultaneously obtained during isothermal melt-crystallization of blends of low-molecular-weight poly(ethylene oxide) (PEO) and poly(methyl methacrylate) (PMMA). The analysis of results shows that the originally homogeneous, single-phase polymer blend separates into two phases. The PMMA molecules diffuse from the blend and form completely segregated regions while PEO starts to crystallize. The first and dominating effect at the beginning of crystallization is the formation of unstable lamellae of nonintegrally folded chains (NIF). The real-time crystallinity and density of the PEO crystalline phase in absolute units were obtained from the time-resolved SAXS/WAXS results. The structure development proceeds in two steps. A very fast evolution of PEO crystals from the melt starts to crystallize in disordered NIF lamellae with thick amorphous interlayers and with a lower density of crystalline phase. The steep growth of crystallinity and crystalline density mean quick thickening of crystalline part of lamellae and improvement of their crystalline structure. In the second step, the structure of the crystalline phase gradually improves and crystallinity grows very slowly. The recrystallization of NIF lamellae into extended chain lamellae (EC) and lamellae with once folded chains (1F) proceeds during both stages of crystallization. 相似文献
50.
H. Qi D.L. Wang L.M. Ruan 《Journal of Quantitative Spectroscopy & Radiative Transfer》2011,112(15):2507-2519
Particle Swarm Optimization (PSO) algorithms, including standard PSO, Stochastic PSO, and Multi-Phase PSO, are applied to solve the time-domain inverse transient radiation problems in the present research. Time-resolved transmittance and reflectance signals of four different measuring models serve as the measurement data, which estimate absorption, scattering coefficients, and geometric position within one-dimensional non-homogeneous media by inverse simulation. To check retrieval performances and accuracies of PSO-based approaches, four different inverse transient radiation cases are investigated to deal with one homogeneous layer, two-layer, three-layer, and continuous non-homogenous media. The influences of different searching ranges, swarm sizes, and maximum fly velocities on the fitness function of PSO are discussed. Meanwhile, the effects of measurement errors on the reconstruction accuracy are also investigated. All the results confirm that radiative parameters could be estimated accurately with measurement noise using PSO-based approaches. 相似文献