Preparation of a novel light-sensitive copolymer and its application in recycling aqueous two-phase systems

Aqueous two-phase systems (ATPSs) are potential bioseparation techniques in industry. However, a key problem is that aqueous two-phase systems could not be effectively recycled to result in high cost and environment pollution. Recently, how to prepare recycling copolymers forming aqueous two-phase systems is focused on in the area. In this study, a light-sensitive copolymer (P(NNC)) was synthesized by using N-isopropylacrylamide (NIPA), N-vinyl-2-pyrrolidone (NVP), chlorophyllin sodium copper salt (CHL) as monomers. The copolymer P(NNC) can form ATPSs with another novel pH-sensitive copolymer (P(ADB)) which was synthesized by co-worker in our laboratory. Over 98% of the P(NNC) copolymer could be recovered by using laser radiation at 488nm. The copolymer P(ADB) could be recovered by adjusting the isoelectric point (pI) to 4.1, with a recovery of 97%. Bovine serum albumin (BSA) and Tyr were partitioned in the P(NNC)-P(ADB) aqueous two-phase systems to examine the systems. It was found that partition coefficient of BSA and L-Tyr could reach 4.1 and 0.12 in the systems, respectively.     

垂直落球粘度计

介绍垂直落球粘度计的结构原理,进行了不确定度分析,并利用国家一级标准物质进行了验证。     

Theoretical studies of insertion reactions of singlet germylene into aryl C-C1 bond of 1-chlorobenzene

The insertion reactions of germylene into C-Cl bond of 1-chorobenzene have been explored using density functional theory. Five germylene species have been chosen for systematically studying.All the stationary points were determined at the B3LYP/ 6-31 1+G(d,p) level of the theory.The results show that,the smaller the△EsT of germylene,the lower the barrier height,and the electropositive substituents on the germylene can increase the reaction activity and exothermicity of insertion into C-Cl bond of 1-choro...     

A new ceramide from transgenic crown galls of Panax quinquefolium

A new ceramide(1) was isolated from transgenic crown galls of Panax quinquefolium.The structure was elucidated as(2S,3S, 4R,20E)-2-[(2′R)-2′-hydroxylpalmitoylamino]- 20- hexacosene- 1,3,4-triol on the basis of spectroscopic and chemical methods.     

基于磺酸酯的荧光探针用于活细胞内过氧化氢的成像检测

基于过氧化氢（H2O2）特异性催化水解磺酸酯,设计合成了新型绿色荧光探针：荧光素二磺酸酯（FS—1）和二氯荧光素二磺酸酯（FS-2）两种螺环内酯型化合物,用于活细胞内过氧化氢的检测．探针结构由元素分析、IR、^1H NMR及^13C NMR表征．实验表明：探针FS-1和FS-2在模拟生物体系中检测过氧化氢具有良好的选择性和灵敏度,且线性范围较宽．细胞成像显示：探针FS-1和FS-2用于PMA刺激或外加不同浓度H2O2孵育的小鼠腹膜巨噬细胞均呈现明亮的绿色荧光,且能对细胞内H2O2微摩尔级浓度变化产生响应,证明两探针均具有良好的膜渗透性、高的选择性及良好的灵敏度．该方法的建立对研究生物体内H2O2的产生,H2O2导致的各种疾病机制及H2O2介导的信号转导途径具有重要的理论及实际意义．     

岩石节理(断裂)表面的多重分形性质

提出了新的投影覆盖法———ＰｒｏｊｅｃｔｉｖｅＣｏｖｅｒｉｎｇＭｅｔｈｏｄ（ＰＣＭ），并结合无接触激光表面测量技术直接测定出断裂表面分维值Ｄ∈［２，３）．建立了表面粗糙性投影覆盖概率函数，分析了节理（断裂）表面的多重分形性质．     

条形空腔药包破坏范围和抛掷堆积规律的研究

本文讨论了条形空腔药包的破坏范围和抛掷、堆积规律,以及多层多排布药方案分段起爆时,后排药包临空面及最小抵抗线的确定方法。     

分数算子描述的黏弹性体力学问题数值方法

讨论由黎曼-刘维尔 (Riemann-Liouville)分数导数描述的黏弹性体力学问题的数 值方法. 该方法利用黎曼-刘维尔分数 导数定义中核函数的特性，并结合被积函数在单步中的逼近以及Newmark型数值法，建立 了分数导数计算公式. 算例表明，该算法具有收敛快、精度高、稳定性好和易于 应用和改进的优点. 在对动态系统的瞬态响应分析和有限元分析格式中，算法都获得了 满意的结果.     

MORSE程序中的MonteCarlo技巧扩充

扩充了MORSE程序的MonteCarlo技巧,针对球几何穿透概率计算,引入了三种MonteCarlo技巧,增强了MORSE程序处理屏蔽问题的能力和计算可靠性。