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21.
A novel strategy for selective collection and detection of breast cancer cells (MCF-7) based on aptamer–cell interaction was developed. Mucin 1 protein (MUC1) aptamer (Apt1) was covalently conjugated to magnetic beads to capture MCF-7 cell through affinity interaction between Apt1 and MUC1 protein that overexpressed on the surface of MCF-7 cells. Meanwhile, a nano-bio-probe was constructed by coupling of nucleolin aptamer AS1411 (Apt2) to CdTe quantum dots (QDs) which were homogeneously coated on the surfaces of monodispersed silica nanoparticles (SiO2 NPs). The nano-bio-probe displayed similar optical and electrochemical performances to free CdTe QDs, and remained high affinity to nucleolin overexpressed cells through the interaction between AS1411 and nucleolin protein. Photoluminescence (PL) and square-wave voltammetric (SWV) assays were used to quantitatively detect MCF-7 cells. Improved selectivity was obtained by using these two aptamers together as recognition elements simultaneously, compared to using any single aptamer. Based on the signal amplification of QDs coated silica nanoparticles (QDs/SiO2), the detection sensitivity was enhanced and a detection limit of 201 and 85 cells mL−1 by PL and SWV method were achieved, respectively. The proposed strategy could be extended to detect other cells, and showed potential applications in cell imaging and drug delivery. 相似文献
22.
Comparison of the performance of three ion mobility spectrometers for measurement of biogenic amines
The performance of three different types of ion mobility spectrometer (IMS) devices: GDA2 with a radioactive ion source (Airsense, Germany), UV-IMS with a photo-ionization source (G.A.S. Germany) and VG-Test with a corona discharge source (3QBD, Israel) was studied. The gas-phase ion chemistry in the IMS devices affected the species formed and their measured reduced mobility values. The sensitivity and limit of detection for trimethylamine (TMA), putrescine and cadaverine were compared by continuous monitoring of a stream of air with a given concentration of the analyte and by measurement of headspace vapors of TMA in a sealed vial. Preprocessing of the mobility spectra and the effectiveness of multivariate curve resolution techniques (MCR-LASSO) improved the accuracy of the measurements by correcting baseline effects and adjusting for variations in drift time as well as enhancing the signal to noise ratio and deconvolution of the complex data matrix to their pure components. The limit of detection for measurement of the biogenic amines by the three IMS devices was between 0.1 and 1.2 ppm (for TMA with the VG-Test and GDA, respectively) and between 0.2 and 0.7 ppm for putrescine and cadaverine with all three devices. Considering the uncertainty in the LOD determination there is almost no statistically significant difference between the three devices although they differ in their operating temperature, ionization method, drift tube design and dopant chemistry. This finding may have general implications on the achievable performance of classic IMS devices. 相似文献
23.
Different-shaped aluminas were readily prepared via hydrothermal reaction. It was found that the morphology and the electrochemical sensing properties of alumina were heavily dependent on the reaction time. When extending the reaction time from 6 h to 24 h, the obtained alumina samples changed from amorphous bumps to regular microfibers in diameter of 200 nm, as confirmed by scanning electron microscopy. Transmission electron microscopy observation revealed that longer reaction time was beneficial for the formation of porous and uniform fiber-like structures. Electrochemical tests proved that alumina microfibers were more active for the oxidation of amaranth and exhibited much higher enhancement effect, compared with alumina bumps. On the surface of alumina microfibers, the oxidation peak currents of amaranth increased remarkably. The influences of pH value, amount of alumina microfibers, and accumulation time on the signal enhancement of amaranth were discussed. As a result, a novel electrochemical method was developed for the detection of amaranth. The linear range was from 1 to 150 nM, and the detection limit was 0.75 nM after 1-min accumulation. The analytical application in drink samples was investigated, and the results consisted with the values that obtained by high-performance liquid chromatography. 相似文献
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为了抑制蛟龙号载人潜水器水声通信受母船色噪声干扰的影响,本文提出了一种统计匹配滤波器在色噪声环境下使用的新方法,它通过将接收信号映射到一个生成子空间中进行信号检测,可提高输出水声通信信号的信噪比。受客观条件限制,向阳红09船——蛟龙号载人潜水器的试验母船噪声环境比较恶劣,对水声通信产生了较强的干扰。如何在向阳红09船的这种强、色噪声环境下,提升水声同步信号的检测能力,对提高水声通信的可靠性是非常有帮助的。实际采集试验数据分析结果验证了该检测算法比传统匹配滤波器有更强的信号检测能力。 相似文献
26.
新一代运载火箭时序仿真系统具有数字电路速度快、集成度高的特点,系统要求发出多路高精度时序、时串信号以满足新一代运载火箭地面测试设备的检查与校准需求,因此信号完整性问题在系统设计中不容忽视。针对仿真系统的典型模块(USB 3.0 Super-speed差分线、FPGA外设数据走线、时钟走线)进行建模分析仿真得出PCB硬件电路设计参数,给出时序仿真系统设计信号完整性问题的抑制和解决方法,优化了板级信号质量,改善系统可靠性、工作连续性和输出精度,可有效提高新一代运载火箭测试效率和测试可靠性。 相似文献
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噪声等效温差(NETD)是表征红外成像系统灵敏度的关键参数,也是评估红外成像系统性能的重要参数之一,应用广泛。通过对红外成像系统3D噪声、信号传递函数(SiTF)、NETD等参数测试方法、算法和流程研究,给出一种基于视频文件的3D噪声离线对比测试方法和一种SiTF线性区自动判断计算斜率算法,在此基础上针对某型技术保障装备国产化光电检测平台开发了配套应用软件功能模块。实现了通过计算单位均方根噪声所对应的SiTF斜率值,分析得出系统NETD参数值的功能。以某型热像仪为被测对象,开展了测试结果重复性和一致性试验,并与美国Optikos公司I-SITE红外整机测试系统进行了对比测试。实验结果表明开发的NETD参数测试功能模块测试精度和重复性满足设计要求,具有较高实用价值,已进行了工程应用。 相似文献
30.
Small target detection is one of the major concern in the development of infrared surveillance systems. Detection algorithms based on Gaussian target modeling have attracted most attention from researchers in this field. However, the lack of accurate target modeling limits the performance of this type of infrared small target detection algorithms. In this paper, signal to clutter ratio (SCR) improvement mechanism based on the matched filter is described in detail and effect of Point Spread Function (PSF) on the intensity and spatial distribution of the target pixels is clarified comprehensively. In the following, a new parametric model for small infrared targets is developed based on the PSF of imaging system which can be considered as a matched filter. Based on this model, a new framework to boost model-based infrared target detection algorithms is presented. In order to show the performance of this new framework, the proposed model is adopted in Laplacian scale-space algorithms which is a well-known algorithm in the small infrared target detection field. Simulation results show that the proposed framework has better detection performance in comparison with the Gaussian one and improves the overall performance of IRST system. By analyzing the performance of the proposed algorithm based on this new framework in a quantitative manner, this new framework shows at least 20% improvement in the output SCR values in comparison with Laplacian of Gaussian (LoG) algorithm. 相似文献