Comparison of the Contributions of Tetrahydrofurfuryl Alcohol and PEG to a-Chymotrypsin Renaturation with High Performance Hydrophobic Interaction Chromatography

Theinvestigationofproteinrenaturation,orrefoldinghasbecomeaveryhotpointinbothliquidchromatography(LC)andlifesciencefields.Besidesusualdilutionanddialysismethods,manynewmethodsconcernproteinrenaturationhavebeenpresented,suchasreversemicelles1,chaperones2,polyethyleneglycol(PEG)3,surfactant4andantibodies5etc.Oneoftheauthorssuggestedhighperformancehydrophobicinter-actionchromatography(HPHIC)tobeanewtoolforproteinrenaturation6.Areviewonproteinrenaturationwithliquidchromatography(LC)wasrecentl…     

Study on the Synthesis of Metabolite of Clausenamide （CM1）

(-) Clausenamide (1) showed strong nootropic action, while its (+) antipode had no such an action. The content of CM1(2) in the metabolites of (-)1 is much higher than that of (+)11. For comparing the nootropic activity of enantiomers, (+) and (-) CM1 were synthesized. CM1 is the hydroxylated product of the N-methyl group of clausenamide. Oxidation of the C3-OH and C6-OH protected clausenamide to introduce the hydroxyl group into the methyl was tried but unsuccessful. Then de novo s…     

Studies on Hepatocyte-Targeting Magnetic Resonance Imaging Macromolecular Contrast Media

ＳｔｕｄｉｅｓｏｎＨｅｐａｔｏｃｙｔｅ┐ＴａｒｇｅｔｉｎｇＭａｇｎｅｔｉｃＲｅｓｏｎａｎｃｅＩｍａｇｉｎｇＭａｃｒｏｍｏｌｅｃｕｌａｒＣｏｎｔｒａｓｔＭｅｄｉａ＊ＦＵＹａｎ－ｊｕｎａｎｄＺＨＵＯＲｅｎ－ｘｉ＊＊（ＤｅｐａｒｔｍｅｎｔｏｆＣｈｅｍｉｓｔ...     

STUDY ON THE SYNTHESIS AND PHYSICOCHEMICAL PROPERTIES OF SUCROSE POLYESTER

1. INTRODUCTIONSucrose polyester (SPE) is neither metabolized nor absorbed, it makes no caloric contribution to the diet. Excessive dietary fat intake is associated with increasing risk of obesity, high blood cholesterol, hypertension, coronary heart disease and some types of cancer. At present, fat substitutes are fashionable all over the world. SPE is a new functional fat substitute, which is similar to ordinary oils in physical and chemical properties. In a word, the research and dev…     

Electrochemical Enzyme Immunoassay of Tumor Marker CA15-3 with Capillary Electrophoresis

CA15-3 is a circulating antigen that is relatively specific for human breast tissue. CA15-3 is significantly more sensitive than carcinoembryonic antigen in the evaluation of patients with both primary and metastatic breast cancer. CA15-3 levels are often measured by using radioimmunoassay and enzyme immunoassay (EIA) in clinic. These methods are carried out manually. Tedious processes, slow reaction, poor reproducibility are their major problems. Capillary electrophoretic immunoassay …     

Positional and Quantitative Characterization of the Hydroxyethyl Groups in Hydroxyethyl Starch by GC/MS or NMR

Hydroxyethyl starches (HES) have been used medically as plasma volume expanders. They are composed of a starch backbone and substituted hydroxyethyl groups. The backbone is ?(1,4)-glycosidic-linked anhydroglucose units, which has branches formed by ?(1,6)-glycosidic bonds. Hydroxyethylation can take place at the C-2, C-3 and C-6 sites of the glucose rings, as well as at the hydroxyl groups of the substituted hydroxyethyl groups. Variation of the position and quantity of the substituted …     

Study on the Surface Photovoltage and Fluorescence Properties of N, N-Bis(4-aminopheny)-1, 4 quinonenediimine Doped with H_4SiW_(12)O_(40)

Polyaniline, especially its acid-dopping composite material, is a kind of important photoelectric material due to its potential application in many fields1~3. However, it seems to be a problem that the polymers exhibit several deficiencies, including chemical and structural imperfections as a result of mislinkage, saturated sites, molecular weight distribution, the presence of the end groups and conformation defects. All of these defects affect the device efficiency and make it difficult to …     

Inhibitory Effect of Anti-HER-2 Anti-CD3 Bi-specific Antibody on the Growth of Gastric Carcinoma

Introduction Gastriccancerisoneofthemostcommonlyen counteredmalignantdiseasesworldwide,especiallyin AsiaandAfrica[1].Thecombinationofoperation,chemotherapyandradiotherapyisusedfortreatinggas triccarcinoma.However,the5yearsurvivalrateof patientsofgastricca…     

Fabrication and characterization of multidimensional tungsten oxide networks

Approaches for preparation of porous oxides with different length scales are currently available[1?3]. Materials with hierarchical pore systems at two or three different length scales have also been prepared[4?7]. Such materials are important both for the…     

Carbon Fiber-gold/mercury Dual-electrode Detection for Capillary Electrophoresis

Capillary electrophoresis (CE) has become a useful and powerful separation technique and has many applications. Electrochemical detection (ED) has been shown to be one of the most sensitive detection techniques available for use in CE. Dual-electrode detection can expand the applicability of CE-ED1-5, the materials such as gold/mercury1,3, carbon fiber2, Pt4 and Au3,5 have been used for the two electrodes in dual-electrodes. As is well known, the electrochemical response of different c…