The Functional Linkage Among the "ZSL"-Spinal Dorsal Horn-SN

Electrical stimulation of the Zusanli point (ZSL) and solitary tract nucleus (SN) as well as microelectrode recording from the laminae Ⅲ-Ⅴ of the lumbar spinal dorsal horn have been used on the pentobarbital anesthetized rats, finding and identifying 57 spinal neurons responding to the stimulations of both ZSL and SN. Among them, 34 responded antidromically to SN; the others responded orthodromically to SN. Among them, the lowthreshold mechano-receptive(LTM) neurons and wide-dynamic-range (WDR) neurons were 50 percent respectively. The results indicate that (ⅰ) a single spinal dorsal horn neuron receives somatic afferent input and then conveys it to the visceral sensory nucleus-SN; (ⅱ) some spinal dorsal horn neurons receive, in turn, innervation from the SN; (ⅲ) the convergence and integration between somatic and visceral sensory inputs might occur in the spinal dorsal horn neurons and/or SN.     

微波消解-非萃取分光光度法测定聚乙烯包材中的锑

建立聚乙烯包材中锑含量的测定方法。微波消解法消解处理聚乙烯包材后,利用表面活性剂OP对5-Br-PADAP、碘化钾和锑的三元复合物的增溶作用,采用络合分光光度法测定锑的含量,筛选了分光光度法测定锑含量的显色条件。锑质量浓度(0.100～1.00 mg·L~(-1))与锑吸光度呈良好的线性关系,加标回收率为103.3～104.0%,精密度RSD小于2%,与标准物质和国标方法相比较,该方法的准确性得到了验证。本方法简单、准确性高,精密度好,可用于聚乙烯包材中锑含量的测定。     

THE PROJECTION LINKAGE BETWEEN THE SPINAL DORSAL HORN NEURONS AND BOTH THE SOLITARY TRACT AND DORSAL COLUMN NUCLEI

Electrical stimulation of the solitary tract nucleus (SN) and dorsal column nuclei (DCN)as well as microelectrode recording from the lumbal spinal dorsal horn have been used tofind and identify the axonal projection of and the afferent innervation on the spinal neuronsof pentobarbital- anesthetized rats. A total of 92 neurons was recorded and identified mainly in laminae Ⅲ-Ⅴ of the lumbarspinal dorsal horn. Of them, 38 neurons were activated antidromically from stimulation ofboth the SN and DCN. The other 54 neurons responded synaptically to both the SN andDCN stimulations. The initial antidromic responses of 8 neurons in the first group werefollowed by one or more responses synaptically driven from the SN and/or DCN stimulation.Conduction velocities were in the range of A delta fibers, but faster in the antidromicresponses and slower in the synaptic responses. These results indicate that (i) some spinal neurons issue branched axons of larger-sizedA delta fibers and double project to both the SN and D     

脊髓背角神经元同孤束核及背索核的投射联系

在以戊巴比妥钠麻醉的大鼠上,应用电刺激孤束核(SN)和背索核(DCN)以及腰髓背角微电极记录技术,发现并鉴定脊髓神经元的轴突投射和传入支配。在腰髓背角Ⅲ—Ⅴ板层共记录和鉴定了92个神经元。其中38个神经元可被SN和DCN双重地逆向激动;其余54个神经元既对SN又对DCN的刺激发生突触反应;8例发生双重逆向反应的神经元还对SN和/或DCN的刺激发生突触反应。逆向和突触反应的传导速度均属Aδ纤维,但前者较快,后者较慢。结果提示,(1)有些脊髓神经元发出较粗的Aδ分叉轴突,向SN和DCN进行双投射;(2)这种双投射神经元有的还从SN和/或DCN接受较粗Aδ纤维支配;(3)另一些脊髓神经元受来自SN和DCN较细Aδ纤维的双重支配。