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Yasuhiro Tanaka Tatsuro Endo Yasuko Yanagida Takeshi Hatsuzawa 《Microchemical Journal》2009,91(2):232-238
In this paper, we describe the design and fabrication of a dielectrophoresis (DEP)-based cell-positioning and cell-culture device for the construction of cell networks. This device enables both individual cell positioning and cell culture. Titanium electrodes were fabricated by deposition. Furthermore, microchambers and microchannels composed of SU-8, which is a negative photoresist, were used to carry out cell culture and enable cell differentiation. Using our device, N1E-115 cells were individually positioned in the microchambers, and the positioning yield was 45%. After positioning, the cells could be continuously cultured in the microchambers. Furthermore, the cells differentiated, and their neurites extended through the microchannels after cultivation for several days. These results indicate that our device greatly increases the prospects for individual cell positioning and can be used to construct cell networks that have several applications in the medical field, for example, in drug screening. 相似文献
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An immiscible liquid–liquid multiphase flow in a cross‐junction microchannel was numerically studied using the lattice Boltzmann method. An improved, immiscible lattice BGK model was proposed by introducing surface tension force based on the continuum surface force (CSF) method. Recoloring step was replaced by the anti‐diffusion scheme in the mixed region to reduce the side‐effect and control the thickness of the interface. The present method was tested by the simulation of a static bubble. Laplace's law and spurious velocities were examined. The results show that our model is more advantageous for simulations of immiscible fluids than the existing immiscible lattice BGK models. Computational results of multiphase flow in a cross‐junction microchannel were obtained and analyzed based on dimensionless numbers. It is found that the flow pattern is decided mostly by the capillary number at a small inlet flux. However, at the same capillary number, a large inlet flux will lead to much smaller droplet generation. For this case, the flow is determined by both the capillary number and the Weber number. Copyright © 2007 John Wiley & Sons, Ltd. 相似文献
14.
John Walton 《Surface and interface analysis : SIA》2007,39(4):337-342
Localised corrosion on a microchannel plate has been studied using X‐ray photoelectron spectromicroscopy. Curve fitting to the spectra at each pixel in the image data set reveals the presence of sodium and carbonate species at the corrosion sites, which optical microscopy suggests originated as droplets. It is proposed that aerosol contamination of the alkali enriched microchannel plate during removal of particulate material using a stream of compressed gas was responsible for the initiation of corrosion. Copyright © 2006 John Wiley & Sons, Ltd. 相似文献
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低温冷冻靶是实现惯性约束聚变(inertial confinement fusion,ICF)的关键部件之一.低温靶靶丸内杂质气体的去除程度和效率对低温靶燃料冰层的在线制备具有重要意义.依据低温靶物理对冰层杂质含量的设计要求,在计算靶丸内杂质气体最大允许分压的基础上,建立了靶丸内气体在微米级充气管内流动的抽空流洗模型.模拟研究了不同微管尺度及结构、温度对靶丸内杂质气体抽空流洗效率的影响规律,获得了靶丸充气微管的最佳管型设计方案.基于最佳管型设计,优化得到了具有最高抽空流洗效率的抽空时间与流洗次数组合策略. 相似文献
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叙述微通道板的噪声,着重介绍微通道板固定图案噪声及其简便新颖而又实用的一种测试方法,给出测试结果并进行分析。 相似文献
18.
Poisson-Boltzrnann equation for EDL (electric double layer) and Navier-Stokes equation for liquid flows were numerically solved to investigate resistance effect of electric double layer on liquid flow in microchannel. The dimension analysis indicates that the resistance effect of electric double layer can be estimated by an electric resistance number, which is proportional to the square of the liquid dielectric constant and the solid surface zeta potential, and inverse-proportional to the liquid dynamic viscosity, electric conductivity and the square of the channel width. An "electric current density balancing" (ECDB) condition was proposed to evaluate the flow-induced streaming potential, instead of conventional "electric current balancing" (ECB) condition which may induce spurious local backflow in neighborhood of the solid wall of the microchannel. The numerical results of the flow rate loss ratio and velocity profile are also given to demonstrate the resistance effect of electric double layer in microchannel. 相似文献
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A new method of pumping a fluid through a microchannel device using a gas bubble-piston, set in motion by the thermocapillary force induced by a light beam, is proposed. To demonstrate the method, a model micropump has been assembled. The model consists of two reservoirs connected by two channels with a bubble-piston driven by a light beam. The pumping rate and the volume per piston stroke are evaluated experimentally. The method proposed is compared with known microfluid pumping methods. Some advantages of the new method are indicated. 相似文献
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A numerical study is reported on the fully developed unsteady laminar fluid flow in microchannel parallel-plates partially filled with a uniform porous medium and partially filled with a clear fluid. The flow is induced by the movement of one of the plates and the pressure gradient. The Brinkman-extended Darcy model is utilized to model the flow in the porous region, while the Stokes equation is used in the clear fluid region. A theoretical analysis is also presented for the fully developed steady flow to find closedform expressions for the interfacial velocity and the velocity and skin frictions at the bounding plates. Numerical computations shows excellent agreement between the closedform solutions for fully developed steady flow and the numerical solution to unsteady flow at large values of time. 相似文献