A proteomic approach based on multiple parallel separation for the unambiguous identification of an antibody cognate antigen

Autoantibodies obtained from cancer patients have been identified as useful tools for cancer diagnostics, prognostics, and as potential targets for immunotherapy. Serological proteome analysis in combination with 2‐DE is a classic strategy for identification of tumor‐associated antigens in the serum of cancer patients. However, serological proteome analysis cannot always indicate the true antigen out of a complex proteome identified from a single protein spot because the most abundant protein is not always the most antigenic. To address this problem, we utilized multiple parallel separation (MPS) for proteome separation. The common identities present in the fractions obtained using different separation methods were regarded as the true antigens. The merit of our MPS technique was validated using anti‐ARPC2 and anti‐PTEN antibodies. Next, we applied the MPS technique for the identification of glycyl‐tRNA synthetase as the cognate antigen for an autoantibody that was overexpressed in the plasma of breast cancer patients. These results reveal that MPS can unambiguously identify an antibody cognate antigen by reducing false‐positives. Therefore, MPS could be used for the characterization of diagnostic antibodies raised in laboratory animals as well as autoantibodies isolated from diseased patients.     

过硫酸钾存在下曲匹布通极谱平行催化波的研究及其应用

采用平行催化波法、循环伏安法等手段研究了在KH2PO4-Na2HPO4介质中过二硫酸钾存在下曲匹布通平行催化波产生的机理。曲匹布通的羰基官能团C=O在KH2PO4-Na2HPO4介质中以2e-,2H+方式极谱还原,产生了单个还原波。加入氧化剂过二硫酸钾时,曲匹布通还原中间体自由基在电极表面上能被过二硫酸钾及其中间还原体硫酸根阴离子自由基氧化,再生为原可还原基团C=O,从而产生了曲匹布通的平行催化波。基于此,提出了一种测定曲匹布通的新方法。     

平行五波长高效液相色谱指纹图谱全息整合法定量鉴定杞菊地黄丸的整体质量

建立了杞菊地黄丸(Qijudihuang Pill, QJDHP)平行五波长(PFW)高效液相色谱(HPLC)指纹图谱,并依据系统指纹定量法结合全息整合法定量鉴定了杞菊地黄丸的整体质量。采用反相HPLC法,以丹皮酚(POL)为参照物峰,分别于203、228、265、280和326 nm下检测,分别确定了51、49、52、49和47个共有指纹峰,建立了QJDHP的PFW-HPLC指纹图谱。分别以权重法、均值法和投影参数法整合5个波长下各样品的定性定量全信息,结果基于5个波长综合信息用系统指纹定量法鉴定11批QJDHP样品,其中有8批质量为好,1批为较好,质量一般为2批。评价时以均值法最为简捷和准确。本实验结果表明,平行多波长指纹图谱整合法是基于从全信息角度整体定性和定量鉴定中药质量的有效可信方法,是对HPLC-二极管阵列检测（DAD）三维指纹图谱的简化定量处理,其整体综合定量鉴定结果具有可靠性。     

基于乘客惰性行为的平行航班动态定价

在同一条航线上,航空公司提供起飞时间不同的多个航班,这样的航班称为平行航班。本文以同一航线上的多个平行航班为研究对象,在考虑乘客的惰性深度和惰性广度的基础上,利用动态规划方法,研究了乘客的惰性行为对各航班最优价格的影响。数值实验表明:乘客的惰性对各航班的最优价格起负面影响,即各航班的最优价格随乘客惰性深度的增加而减小,随乘客惰性广度的增加而减小。     

具有学习效应和加工时间可控的平行机排序问题

本文研究了一类不相关平行机的排序问题,在该问题中工件的加工时间既具有学习效应,又资源可控,也就是说在该问题模型中,工件的实际加工时间为其正常的加工时间、加工过程中工件所处位置以及加工时间可控这些变量的函数。该研究的目的是为使得总机器负载和总的控制费用的加权和最小以及总的完工时间和总的控制费用的加权和最小。文章通过对问题的相关性质的分析和证明找到了一个解决问题的最优化算法,并且也证明了在处理机的数量给定的条件下,该问题的时间复杂性为O(n^m+2),最后也给出了相应的数值例子来阐述该问题。     

An alternative globalization strategy for unconstrained optimization

We propose a new globalization strategy that can be used in unconstrained optimization algorithms to support rapid convergence from remote starting points. Our approach is based on using multiple points at each iteration to build a sequence of representative models of the objective function. Using the new information gathered from those multiple points, a local step is gradually improved by updating its direction as well as its length. We give a global convergence result and also provide the parallel implementation details accompanied with a numerical study. Our numerical study shows that the proposed algorithm is a promising alternative as a globalization strategy.     

A feasible memristive Chua's circuit via bridging a generalized memristor

By bridging a generalized memristor between a passive $LC$ oscillator and an active $RC$ filter, a simple and feasible memristive Chua''s circuit is presented. The generalized memristor without grounded limitation is equivalently achieved by a full-wave rectifier cascaded with a first-order parallel $RC$ filter. The dynamical characteristics of the proposed circuit are investigated both theoretically and numerically, from which it can be found that the circuit has three unstable equilibrium points and demonstrates complex nonlinear phenomena. The experimental circuit is easy to implement and the measurement results validate the results of theoretical analyses.     

A Characterization of the degree sequences of 2‐trees

A graph G is a 2‐tree if G = K₃, or G has a vertex v of degree 2, whose neighbors are adjacent, and G/ v is a 2‐ tree. A characterization of the degree sequences of 2‐trees is given. This characterization yields a linear‐time algorithm for recognizing and realizing degree sequences of 2‐trees. © 2008 Wiley Periodicals, Inc. J Graph Theory 58:191‐209, 2008     

用火焰检测电子管检测火焰的实验方法

介绍了采用新型传感元件GP-16火焰检测电子管实现非接触式火焰检测的方法。并给出了完成其功能的电路图．     

Parallel Spinors and Holonomy Groups on Pseudo-Riemannian Spin Manifolds

We describe the possible holonomy groups of simply connected irreducible non-locally symmetric pseudo-Riemannian spin manifolds which admit parallel spinors.