Anticancer Activity of Lesbicoumestan in Jurkat Cells via Inhibition of Oxidative Stress-Mediated Apoptosis and MALT1 Protease

This study explores the potential anticancer effects of lesbicoumestan from Lespedeza bicolor against human leukemia cancer cells. Flow cytometry and fluorescence microscopy were used to investigate antiproliferative effects. The degradation of mucosa-associated lymphoid tissue lymphoma translocation protein 1 (MALT1) was evaluated using immunoprecipitation, Western blotting, and confocal microscopy. Apoptosis was investigated using three-dimensional (3D) Jurkat cell resistance models. Lesbicoumestan induced potent mitochondrial depolarization on the Jurkat cells via upregulated expression levels of mitochondrial reactive oxygen species. Furthermore, the underlying apoptotic mechanisms of lesbicoumestan through the MALT1/NF-κB pathway were comprehensively elucidated. The analysis showed that lesbicoumestan significantly induced MALT1 degradation, which led to the inhibition of the NF-κB pathway. In addition, molecular docking results illustrate how lesbicoumestan could effectively bind with MALT1 protease at the latter’s active pocket. Similar to traditional 2D cultures, apoptosis was markedly induced upon lesbicoumestan treatment in 3D Jurkat cell resistance models. Our data support the hypothesis that lesbicoumestan is a novel inhibitor of MALT1, as it exhibited potent antiapoptotic effects in Jurkat cells.     

Solvent effects on the structure of the triplet excited state of xanthone: a time‐resolved resonance Raman study

Solvent effects, especially intermolecular hydrogen bonding, play a central role in the photophysics and photochemistry of aromatic ketones. To gain insight into the solute–solvent interactions and their implications for structure and reactivity, we studied xanthone (XT) in two different solvents of similar dipolarity: acetonitrile (ACN; aprotic) and methanol (MeOH; protic), using time‐resolved resonance Raman (TR³) spectroscopy in conjunction with time‐dependent density functional theory calculations. Raman excitation profiles of XT in ACN followed the triplet‐triplet absorption band with a shoulder at the blue end, but for MeOH, they followed the triplet‐triplet absorption band quite closely; therefore, we propose that the resonance enhancement of Raman peaks are from two states in ACN and from a single state in the MeOH solvent. Furthermore, a resonance Raman peak at 614 cm⁻¹ (a₂ symmetry) that appeared in ACN but not in the MeOH solvent has been identified as a vibronic active mode that could be involved in coupling the two lowest 1³ππ* (1³A₁) and 1³nπ* (1³A₂) excited states. This was further confirmed by depolarization ratio measurements of some of the representative TR³ peaks in ACN, which showed a depolarized intensity for the 614 cm⁻¹ peak while the other peaks were polarized. Interestingly, we also observed blue shifting of some of the vibrational frequencies of XT in the 1³ππ* state compared with the ground state with increasing solvent polarity. This anomalous blue shift casts doubt on the general use of the resonance canonical structure to explain the structure of the excited states. In summary, we propose that the different hydrogen bonding mechanisms exhibited by the two lowest triplet states of XT separate them further in energy and that this can contribute to its low reactivity towards H atom abstraction in protic solvents. Copyright © 2016 John Wiley & Sons, Ltd.     

An enhanced polarization mechanism for the metal cations modified amorphous TiO<Subscript>2</Subscript> based electrorheological materials

In the present work, we developed a new kind of electrorheological (ER) materials, metal cations modified amorphous TiO₂ gels. The static yield stress of Sn⁴⁺ modified amorphous TiO₂ gel based ER fluid with a volume fraction = 38% reaches 26.2 kPa at E = 3.5 kV/mm. The result shows that metal cations can significantly enhance the ER activity of amorphous TiO₂ gels. We then proposed a novel ER effect mechanism (metal cations enhanced polarization mechanism) to clarify the experimental results. We believe that it is the metal cations that enhanced the polarization of the polar groups (-OH) which results in the corresponding enhancement of the interfacial polarization.     

Diffusion in layered random flows,polymers, electrons in random potentials,and spin depolarization in random fields

Several related models are studied in a common framework. We first reconsider the model of Matheron and de Marsilly for (anomalous) tracer dispersion in a stratified porous medium. In each horizontal layer the flow velocity is constant, parallel to the layer, and depends randomly on the vertical coordinate z. This model is mapped onto ad=1 localization problem in a random potential and, equivalently, onto ad=1 polymer. At larget theaveraged distribution of horizontal displacementsx takes the scaling form [P(x, t, z=0)]=at ^–5/4 Q(bxt ^–3/4), whereQ(y) is independent of the details of the model.Q(y),a, andb are obtained exactly for a large class of models. From the Lifschitz tails of the localization problem we find in the regionxt ^3/4, i.e.,y, thatQ(y)¦y¦ exp(–C¦y¦^4/3). We also obtain exactly ind=1 the scaling functions for the local and total average magnetization of spins diffusing in a random magnetic field, by mapping onto a polymer problem, as well as the average local concentration for diffusion in the presence of random sources and sinks. These mappings are then used to study higher-dimensional extensions of these models.     

VDF/TrFE共混物晶区相容性的热释电流研究

Ferroelectric blends of vinylidene fluoride（VDF）/ trifluoroethylene（TrFE）copolymer with different molar ratio of 56/44,60/40,63/37,67/33,70/30,73/27 and 77/23 were fabricated by casting from methyl ethyl ketone solution. Wide angle X-ray diffraction shows two peaks（17. 6o and 19. 5o）for all blends and copolymers,associating with amorphous region and（110）（200）reflections,respectively. It indicates that blending does not introduce any new crystal lattice. Analysis of differential scanning calorimetry（DSC）presents that the blends display the phase transition characters of both copolymers of 77/23 and 56/44. The blend components are immiscible in the crystalline phase. Thermally stimulated depolarization current of the blends exhibits the characters of both components at different polarization temperatures. In accordance with DSC results,it also proves that the crystalline phases of 56/44 and 77/23 are just independent. In addition,it is also proved that the space charge can stabilize the remnant polarization to some extent.     

应用于AGV自动导引车的快速充电系统

设计建立一种用于AGV自动导引车镍氢动力蓄电池的快速充电系统,该系统在充电过程中能实时监测蓄电池组的各个运行参数,完成数据运算并逻辑判断、控制充电过程至其结束,并利用去极化放电脉冲提高充电效率.     

激光雷达偏振成像遥感的望远镜系统偏振分析

激光雷达偏振成像主要是利用不同目标散射光偏振度的差异来实现对目标成像的。由于大部分光学系统都存在消偏振效应,因此系统自身的消偏振问题在成像过程中必须考虑。根据近轴理论和消像差的要求设计出卡塞格伦望远镜的几何结构,应用琼斯理论、光波的相干矩阵和菲涅耳反射理论对空气和卡塞格伦望远镜的交界面进行了消偏振分析,并使用Matlab软件仿真了卡塞格伦望远镜在镀金属反射膜前后,反射光偏振度在望远镜径向直径上各点的变化曲线。根据仿真结果可以看出偏振成像系统中使用的卡塞格伦望远镜在镀铝金属反射膜时其消偏振效应在成像过程中可以忽略。     

Quantitative analysis of crystallization kinetics by light depolarization technique. Possibilities and limitations

The kinetics of isothermal crystallization of various polymers was investigated by light depolarization technique (LDT) using the new setup with direct registration of depolarization ratio. Experimental data were analyzed using new method proposed by Ziabicki who shown that degree of crystallinity is a non-linear function of degree of depolarization, crystal thickness, and its birefringence. Other experimental methods were involved providing supplementary information on crystal thickness (SAXS) and allowing comparison of crystallization kinetics (WAXS, DSC). The advantage of LDT relies on high sampling rate allowing on-line measurements and lack of inertia effects that exist in other methods like calorimetry. The limitations of the applicability of the method are discussed. The method needs supplementary information not only on crystal thickness but also on variable optical birefringence of real crystals. Our results show that LDT can be used in a simple way for investigation of crystallization kinetics at relatively high temperatures, providing large and perfect crystals. In such a case it is sufficient to use crystal intrinsic birefringence and final crystal thickness typical at particular temperature of crystallization. On the other hand, depolarization ratio combined with measurements by other methods (crystallinity and crystal thickness) can be used for estimation of crystal birefringence.     

高功率隔离器单模与多模工作退偏特性

以传统结构隔离器为模型,应用琼斯理论,比较分析了单模与多模激光入射时高功率隔离器热致退偏特性。研究结果表明:高功率隔离器热致退偏可分为线性双折射致退偏与圆双折射致退偏;两种退偏均与入射激光功率的平方成正比;在光功率一定的条件下,线性双折射致退偏不随光斑半径的变化而变化,圆双折射致退偏随光斑半径的增大而减小;在其它条件相同的条件下,多模光场能够有效地降低隔离器热致退偏,多模时线性双折射致退偏仅为单模时退偏的60%,圆双折射致退偏也比单模时要小,减小幅度随光斑半径的增加而增加。     

一种斜入射F-P型薄膜滤光片消偏振设计方法

薄膜滤光片在斜入射使用时,s偏振光和p偏振光的特性会产生分离:两偏振光的中心波长不一致,且s偏振光的通带宽度要小于p偏振光.而采用多种材料且满足特定条件的膜系结构,可使角度入射滤光片两偏振方向的特性趋于一致.通过理论分析,建立了两偏振光反射率的表达式,由表达式可看出非偏振的条件.通过计算,求出相应材料折射率值,从而设计出消偏振的膜系.对一个三腔129层实例膜系进行了计算求解、仿真分析及误差分析.最后的结果验证表明此方法是可行的.