Trapping and releasing of single microparticles and cells in a microfluidic chip

A microfluidic device was designed and fabricated to capture single microparticles and cells by using hydrodynamic force and selectively release the microparticles and cells of interest via negative dielectrophoresis by activating selected individual microelectrodes. The trap microstructure was optimized based on numerical simulation of the electric field as well as the flow field. The capture and selective release functions of the device were verified by multi-types microparticles with different diameters and K562 cells. The capture efficiencies/release efficiencies were 95.55% ± 0.43%/96.41% ± 1.08% and 91.34% ± 0.01%/93.67% ± 0.36% for microparticles and cells, respectively. By including more traps and microelectrodes, the device can achieve high throughput and realize the visual separation of microparticles/cells of interest in a large number of particle/cell groups.     

Development and validation of a forensic multiplex InDel assay: The AGCU InDel 60 kit

Marker sets based on insertion/deletion polymorphisms (InDels) combine the characteristics of both short tandem repeats (STRs) and single nucleotide polymorphisms and have served as effective complementary or stand-alone systems for human identification in forensics. We developed a novel multiplex amplification detection system, designated the AGCU InDel 60 kit, containing 57 autosomal InDels, 2 Y-chromosomal InDels, and the amelogenin locus and validated the kit in a series of studies, which included tests of the PCR conditions; tests for sensitivity, species specificity, reproducibility, stability, and mock case samples; degradation studies; and a population study. The results indicated that the AGCU InDel 60 kit was accurate, specific, reproducible, stable, and robust. Complete DNA profiles were obtained even with 125 pg of human DNA. In tests of artificially degraded samples, we found that the number of alleles detected by the validated kit was considerably greater than that detected by the STR-based AGCU 21+1 kit, even as the degree of degradation increased. Additionally, 564 unrelated individuals from three Han groups were investigated using this novel system, and the values of combined power of discrimination and combined power of exclusion were not less than 1–4.9026 × 10⁻²⁴ and 1–3.1123 × 10⁻⁵, respectively. Thus, the results indicated that the novel kit was more powerful than the previous version of the InDel kit (the AGCU InDel 50 kit). Our results suggest that the AGCU InDel 60 kit can serve as an efficient tool for human forensics and a supplementary kit for population genetics research.     

Algebraic logoi

We introduce normal cores, as well as the more general action cores, in the context of a semi-abelian category, and further generalise those to split extension cores in the context of a homological category. We prove that, if the category is moreover well-powered with (small) joins, then the existence of split extension cores is equivalent to the condition that the change-of-base functors in the fibration of points are geometric. We call a finitely complete category that satisfies this condition an algebraic logos. We give examples of such categories, compare them with algebraically coherent ones, and study equivalent conditions as well as stability under common categorical operations.     

量子相空间分布函数与压缩相干态表示间的变换关系

基于Husimi算符具有压缩相干态投影子形式, 首先介绍了一个新的量子算符表示, 即压缩相干态表示.当高斯展宽参数κ = 1时, 该函数约化为通常的P函数. 作为例子, 研究了热态的压缩相干态表示, 通过图示说明了压缩相干态表示与P函数的区别. 为更好地在量子光学问题中使用该表示, 我们揭示了压缩相干态表示与Wigner函数、Q函数以及Husimi函数间的积分变换关系.     

Sorting algorithms for single‐particle imaging experiments at X‐ray free‐electron lasers

Modern X‐ray free‐electron lasers (XFELs) operating at high repetition rates produce a tremendous amount of data. It is a great challenge to classify this information and reduce the initial data set to a manageable size for further analysis. Here an approach for classification of diffraction patterns measured in prototypical diffract‐and‐destroy single‐particle imaging experiments at XFELs is presented. It is proposed that the data are classified on the basis of a set of parameters that take into account the underlying diffraction physics and specific relations between the real‐space structure of a particle and its reciprocal‐space intensity distribution. The approach is demonstrated by applying principal component analysis and support vector machine algorithms to the simulated and measured X‐ray data sets.     

部分相干光在大气湍流中的光强闪烁效应

以修正Rytov方法为基础,利用部分相干高斯-谢尔(GSM)光束模型并结合Andrews的唯像闪烁模型,研究部分相干光在大气湍流中的光强闪烁效应。推导出适用于不同湍流情形的部分相干光对数光强起伏方差表达式,系统分析了部分相干光在大气湍流中的光强起伏方差。结果表明:在同等的湍流环境下,部分相干光比完全相干光的光强起伏方差要小;光束相干性越差,光强起伏越小,抗湍流能力越强。在不同大气湍流情形下,光源相干性、大气折射率常数等参数对光强起伏都有不同程度的影响。     

微波场作用下三能级原子系统的无反转光放大

研究了外加微波场作用下三能级原子系统的无粒子数反转放大特性. 通过求解系统的密度矩阵方程得到其探测吸收谱. 结果表明, 介质对探测场的吸收呈现多峰结构, 并且在强微波驱动场的作用下, 特定范围内出现了明显的负吸收(即光放大). 进一步定量分析各能级粒子数布居随作用场强度的变化, 揭示出该三能级原子系统呈现无粒子数反转光放大的规律. 最后, 采用缀饰态理论对探测吸收特性做出了准确的解释.     

Al掺杂对HfO2俘获层可靠性影响第一性原理研究

采用基于MS(Materials Studio)软件和密度泛函理论的第一性原理方法, 研究了HfO₂ 俘获层的电荷俘获式存储器(Charge Trapping Memory, CTM)中电荷的保持特性以及耐擦写性. 在对单斜晶HfO₂中四配位氧空位(V_O4) 缺陷和V_O4 与Al替位Hf掺杂的共存缺陷体(Al+V_O4)两种超晶胞模型进行优化之后, 分别计算了其相互作用能、形成能、Bader电荷、态密度以及缺陷俘获能. 相互作用能和形成能的计算结果表明共存缺陷体中当两种缺陷之间的距离为2.216 Å时, 结构最稳定、缺陷最容易形成; 俘获能计算结果表明, 共存缺陷体为双性俘获, 且与V_O4缺陷相比, 俘获能显著增大; Bader电荷分析表明共存缺陷体更有利于电荷保持; 态密度的结果说明共存缺陷体对空穴的局域能影响较强; 计算两种模型擦写电子前后的能量变化表明共存缺陷体的耐擦写性明显得到了改善. 因此在HfO₂俘获层中可以通过加入Al杂质来改善存储器的保持特性和耐擦写性. 本文的研究可为改善CTM数据保持特性和耐擦写性提供一定的理论指导.     

Optomagnetically Controlled Microparticles Manufactured with Glancing Angle Deposition

Optical trapping and magnetic trapping are common micromanipulation techniques for controlling colloids including micro‐ and nanoparticles. Combining these two manipulation strategies allows a larger range of applied forces and decoupled control of rotation and translation; each of which are beneficial properties for many applications including force spectroscopy and advanced manufacturing. However, optical trapping and magnetic trapping have conflicting material requirements inhibiting the combination of these methodologies. In this paper, anisotropic microscaled particles capable of being simultaneously controlled by optical and magnetic trapping are synthesized using a glancing angle deposition (GLAD) technique. The anisotropic alignment of dielectric and ferromagnetic materials limits the optical scattering from the metallic components which typically prevents stable optical trapping in three dimensions. Compared to the current state of the art, the benefits of this approach are twofold. First, the composite structure allows larger volumes of ferromagnetic material so that larger magnetic moments may be applied without inhibiting the stability of optical trapping. Second, the robustness of the synthesis process is greatly improved. The dual optical and magnetic functionality of the synthesized colloids is demonstrated by simultaneously optically translating and magnetically rotating a magnetic GLAD particle using a custom designed optomagnetic trapping system.