Late‐Stage Peptide Diversification by Bioorthogonal Catalytic CH Arylation at 23 °C in H2O

The step‐economical late‐stage diversification of tryptophan‐containing peptides was accomplished through chemo‐ and site‐selective palladium‐catalyzed C?H arylation under exceedingly mild reaction conditions. Thus, the C?H functionalization occurred efficiently at 23 °C with a catalyst loading as low as 0.5 mol %, and/or in H₂O.     

层间剪切带粒度分形几何特征及工程地质意义

本文对分形几何方法表征土的粒度组成加以了补充, 并用它来描述和表征层间剪切带的粒度组成, 据此将其划分为五个类型。在此基础上较为深入地阐述了其工程地质意义, 并着重讨论了用它来识别层间剪切带所遭受的构造变形破坏程度、演化阶段问题等。     

航空发动机压气机叶片振动频率与温度的关系

以某型航空发动机压气机第六级叶片为例，研究了温度对叶片振动频率的影响。发现由于温度的升高导致了材料弹性模量的下降，而弹性模量的下降又导致了叶片在发动机一定的转速范围内，除一阶振动外，其余各阶振动的动频均小于静频，因此与通常的工程处理方法不同，研究压气机叶片的动频时，应考虑温度的影响。     

香港大屿山残坡积土的残余强度试验研究

粘性土的残余强度是边坡稳定性评价、桩基与土的相互作用机理研究及填土边坡设计中的重要参数。本文在综述大量文献的基础上，结合香港大屿山火山岩风化残坡积土的残余强度试验研究，分析了残余强度的测试方法和影响残余强度的因素。研究结果表明，残余强度与有效法向应力间具有明显的非线性关系；与单剪测试结果相比，多级剪测试结果明显偏高。     

Suspension settings for optimal ride comfort of off-road vehicles travelling on roads with different roughness and speeds

This paper reports on an investigation to determine the spring and damper settings that will ensure optimal ride comfort of an off-road vehicle, on different road profiles and at different speeds. These settings are required for the design of a four stage semi-active hydro-pneumatic spring damper suspension system (4S₄). Spring and damper settings in the 4S₄ can be set either to the ride mode or the handling mode and therefore a compromise ride-handling suspension is avoided. The extent to which the ride comfort optimal suspension settings vary for roads of different roughness and varying speeds and the levels of ride comfort that can be achieved, are addressed. The issues of the best objective function to be used when optimising and if a single road profile and speed can be used as representative conditions for ride comfort optimisation of semi-active suspensions, are dealt with. Optimisation is performed with the Dynamic-Q algorithm on a Land Rover Defender 110 modelled in MSC.ADAMS software for speeds ranging from 10 to 50 km/h. It is found that optimising for a combined driver plus rear passenger seat weighted root mean square vertical acceleration rather than using driver or passenger values only, returns the best results. Results indicate that optimisation of suspension settings using one road and speed will improve ride comfort on the same road at different speeds. These settings will also improve ride comfort for other roads at the optimisation speed and other speeds, although not as much as when optimisation has been done for the particular road. For improved ride comfort damping generally has to be lower than the standard (compromised) setting, the rear spring as soft as possible and the front spring ranging from as soft as possible to stiffer depending on road and speed conditions. Ride comfort is most sensitive to a change in rear spring stiffness.     

S波段微波热致超声成像系统研究

微波热致超声成像技术通过向物体发射微波脉冲, 导致物体吸收电磁波温度迅速升高, 产生瞬时压力波, 从而激发产生超声波信号, 通过传感器对产生的超声波信号进行采集并成像, 最终还原了反映物体吸收电磁波能量特性的图像, 由于此方法兼具了微波成像的高对比性和超声成像的高分辨率特点, 理论上验证了热声成像技术对早期乳腺肿瘤检测的可行性. 本实验兼顾系统成像深度和分辨率, 采用S波段的微波脉冲信号源对物体进行辐射, 利用圆形扫描方式对待测物体进行检测, 同时为了更好的验证成像性能, 本实验同时使用了肿瘤仿体及实际生物组织进行成像实验. 通过实验分析, 验证了该系统对肿瘤仿体和生物组织检测的有效性, 以及系统的高分辨率和高对比度特性, 为早期乳房肿瘤检测提供了进一步的理论支撑.     

Metabolite identification by liquid chromatography-mass spectrometry

Metabolite identification (Met ID) is important during the early stages of drug discovery and development, as the metabolic products may be pharmacologically active or toxic in nature. Liquid chromatography-mass spectrometry (LC-MS) has a towering role in metabolism research.This review discusses current approaches and recent advances in using LC-MS for Met ID. We critically assess and compare various mass spectrometers, highlighting their strengths and limitations. Citing appropriate examples, we cover recent LC and ion sources, isotopic-pattern matching, hydrogen/deuterium-exchange MS, data dependent analyses, MS^E, mass defect filter, 2D and 3D approaches for the elucidation of molecular formula, polarity switching, and background-subtraction and noise-reduction algorithms. A flow chart outlines a comprehensive strategy for Met ID, including a focus on reactive metabolites.     

Proteomic analysis of a spring wheat cultivar in response to prolonged cold stress

Cold represents one of the major abiotic factors influencing plant growth and development worldwide. We analysed the long-term responsiveness of an Iranian spring wheat (cv. Kohdasht) to cold from a proteomic point of view, in order to unravel the molecular mechanisms helping a cold-sensitive cultivar to survive exposure to suboptimal temperatures. Plants were grown at 20 or 4°C until entering the reproductive stage and a cross-comparison on the leaf proteomes was performed. Quantitative analyses on protein alterations occurring upon low-temperature exposure showed a reinforcement in ascorbate recycling (dehydroascorbate reductase, ascorbate peroxidase) and protein processing (proteasome subunit, cysteine proteinase), as well as the accumulation of the enzyme devoted to tetrapyrrole resynthesis (glutamate semialdehyde aminomutase). In contrast, among proteins down-regulated after cold stress, we could identify some key Krebs cycle enzymes (isocitrate dehydrogenase, malate dehydrogenase), together with many photosynthesis-related proteins (oxygen-evolving complex proteins, ATP synthase subunits, ferredoxin NADPH oxidoreductase and some Calvin cycle enzymes). Physiological and biochemical parameters (such as shoot apex dissection, chlorophyll, proline and sugar content determination) sustained proteomics findings allowing the present research to contribute to the current knowledge on these long-term responses, which may be crucial to stress adaptation under field conditions.     

Electrospray ionization ion-trap multiple-stage mass spectrometry of Quillaja saponins

Fifteen identified C-18 fatty acyl-containing saponin structures from Quillaja saponaria Molina have been investigated by electrospray ionization ion-trap multiple-stage mass spectrometry (ESI-IT-MS(n)) in positive ion mode. Their MS(1)-MS(3) spectra were analyzed and ions corresponding to useful fragments, important for the structural identification of Quillaja saponins, were recognized. A few key fragments could describe the structural variations in the C-3 and the C-28 oligosaccharides of the Quillaja saponins. A flowchart involving a stepwise procedure based on key fragments from the MS(1)-MS(3) spectra of these saponins, together with key fragments from these saponins and 13 previously investigated saponins, was constructed for the identification of structural elements in Quillaja saponins. Peak intensity ratios in MS(3) spectra were found to be correlated to structural features of the investigated saponins and is therefore of value for the identification of regioisomers.     

非自治的具有阶段结构和时滞的捕食系统的动力学行为

本文研究了一个非自治的具有阶段结构和时滞的捕食-食饵模型.利用比较原理获得了系统持久性的条件,在假设系统为周期系统的条件下通过构造Liapunov函数证明了正周期解的存在唯一性和全局渐近稳定性.