m-6-m型Gemini双季铵盐表面活性剂与DNA的相互作用

合成了5种m-6-m型Gemini双季铵盐表面活性剂,在对产物结构和表面活性进行分析的基础上,分别采用紫外分光光度法和荧光分光光度法考察了m-6-m型Gemini双季铵盐表面活性剂与DNA的相互作用.结果表明,m-6-m型Gemini表面活性剂的CMC随烷基疏水链的增长呈逐渐下降趋势.几种表面活性剂均没有使DNA的紫外吸收峰发生红移或蓝移现象,说明复合物无嵌插作用或氢键形成,表面活性剂与DNA作用后的吸光度随表面活性剂浓度的增大而增强,当表面活性剂的浓度相同时,吸光度随疏水链的增大而逐渐减弱.Gemini表面活性剂浓度增大导致荧光强度降低,表面活性剂与DNA作用时的猝灭为静态猝灭,随着疏水链长度的增长,荧光猝灭常数降低,表面活性剂与DNA之间的作用力减弱.     

Dynamic Docking and Undocking Processes Addressing Selectively the Outside and Inside of Polymersomes

Increasing complexity and diversity of polymersomes and their compartments is a key issue for mimicking cellular functions and protocells. Thus, new challenges arise in terms of achieving tunable membrane permeability and combining it with control over the membrane diffusion process, and thus enabling a localized and dynamic control of functionality and docking possibilities within or on the surface of polymeric compartments. This study reports the concept of polymersomes with pH‐tunable membrane permeability for controlling sequential docking and undocking processes of small molecules and nanometer‐sized protein mimics selectively on the inside and outside of the polymersome membrane as a further step toward the design of intelligent multifunctional compartments for use in synthetic biology and as protocells. Host–guest interactions between adamantane and β‐cyclodextrin as well as noncovalent interactions between poly(ethylene glycol) tails and β‐cyclodextrin are used to achieve selective and dynamic functionalization of the inner and outer spheres of the polymersome membrane.     

Thioether‐derived Macrocycle for Peptide Secondary Structure Fixation

Recently, we developed methods to stabilize peptides into various secondary structures, including α‐helix, type III turn and β‐hairpin via proper thioether based macrocyclization. These conformationally constrained peptidomimetics confer enhanced biophysical properties and provide a valuable avenue towards clinically‐relevant therapeutic molecules. In this personal account, thioether‐derived macrocyclization methods developed by our group for stabilization of α‐helix, type‐III β turn and β‐hairpin conformations are discussed.     

Effect of water on the retention on diol and amide columns in hydrophilic interaction liquid chromatography

The amount of water adsorbed on polar columns plays important role in hydrophilic interaction liquid chromatography. It may strongly differ for the individual types of polar columns used in this separation mode. We measured adsorption isotherms of water on an amide and three diol‐bonded stationary phases that differ in the chemistry of the bonded ligands and properties of the silica gel support. We studied the effects of the adsorbed water on the retention of aromatic carboxylic acids, flavonoids, benzoic acid derivatives, nucleic bases, and nucleosides in aqueous‐acetonitrile mobile phases over the full composition range. The graphs of the retention factors versus the volume fraction of water in mobile phase show “U‐profile” characteristic of a dual hydrophilic interaction–reversed phase retention mechanism. The minimum on the graph that marks the changing retention mechanism depends on the amount of adsorbed water. The linear solvation energy relationship model suggests that the retention in the hydrophilic interaction liquid chromatography mode is controlled mainly by proton–donor interactions in the stationary phase, depending on the column type. Finally, the accuracy of hydrophilic interaction liquid chromatography gradient prediction improves for columns that show a high water adsorption.     

8×4×2~n设计纯净两因子交互作用成分最大数的界

混水平部分因析设计在各类试验中有广泛应用.纯净效应准则是用于选取最优部分因析设计的重要准则之一.本文考虑含有一个八水平因子、一个四水平因子和若干二水平因子的8×4×2~n混水平设计,给出了分辨度为Ⅲ和Ⅳ的该类混水平设计包含纯净两因子交互作用成分最大数的上界和下界.下界通过构造特定设计而得到.     

桩基承载力计算程序GPILE

介绍了单桩和群桩承载力计算程序 GPILE。该程序利用 P-Y曲线、T-Z曲线和 Q-Z曲线将土体与桩之间的相互作用模拟为一系列非线性弹簧 ,然后通过迭代求解桩的变形和内力。在计算群桩时可以考虑群桩效应。另外 ,还可以在桩基上增加上部结构 ,从而考虑桩基和上部结构的共同作用     

Three-Dimensional Dynamic Contact Problem for an Elliptic Crack Interacting with a Normally Incident Harmonic Compression–Expansion Wave

The paper deals with the three-dimensional dynamic problem for an elliptic crack interacting with a normally incident harmonic compression–expansion wave, considering the contact interaction of the crack faces. An asymmetric solution is obtained using an iteration algorithm developed earlier. Numerical results are presented     

Lifting Surface in Subsonic Unsteady Regime

The paper refers to the solution of the integral equation for the acceleration (or pressure) potential for the study of subsonic linearized unsteady flow in view of aeroelastic applications. The case considered is relevant to a trapezoidal wing infinitely thin surface without discontinuities. As is well known [1, 2], the kernel of the integral equation exhibits three singularities, two of which are integrable in elementary form, whereas, for the third one integration in principal part according to Hadamard's rule is necessary. The kernel is therefore reworked in such a way that all the singularities are separated from the regular part, and eventually the discretization is performed in such a way that only the regular part is to be recalculated for each new value of the reduced frequency. Convergence tests, comparison with other methods of solution, and time saving associated with the technique of separation are also shown.Sommario. II lavoro tratta la risoluzione del problema relativo alla equazione integrale nel potenziale di accelerazione (o di pressione) per lo studio di una corrente subsonica linearizzata nonstazionaria, in vista di applicazioni aeroelastiche. Il caso considerato è quello di una superficie alare a pianta trapezoidale in assenza di discontinuità di spessore infinitesimo. Come è noto [1, 2], il nucleo della equazione integrale in parola presenta tre singolarità, due sole delle quali sono integrabili in forma elementare, (o riconducibili ad essa), mentre per la terza è necessario far ricorso alla integrazione in parte principale alla Hadamard. Il nucleo stesso viene quindi rielaborato in modo da isolare tutte le singolarità dalla componente regolare del nucleo; si procede così alla discretizzazione dell' equazione integrale, e, per ogni valore della frequenza ridotta, va ricalcolata solo la parte regolare della matrice risolvente. Vengono poi effettuati tests di convergenza, confronti con altri metodi di soluzione, analisi sui tempi di calcolo e risparmio di tempo di calcolo dovuto alla tecnica di separazione.     

跨声速激波边界层相互作用的被动控制

运用激波管风洞在Ｒ＿（ｅ∞／ｍ＝３×１０￣７，Ｍ＿∞＝０．７３２－０．８１７范围内，在厚度比为１２％的圆弧翼型半模型和厚度比为１４％的超临界翼型半模型上，对被动控制现象及其若干规律进行了实验研究。结果表明，不同空腔深度的开孔壁和导管连通壁均可对壁面激波与边界层的相互作用实现被动控制，使得沿以上两种模型表面的马赫数峰值、逆压力梯度和激波强度明显减小。这对于飞行器将起到减阻作用，如将这一原理和方法用于超、跨声速压气机内激波与边界层相互作用的控制，将提高压气机的效率和工作的稳定性。