用峰鉴别技术反相高效液相色谱法测定火锅汤料中微量吗啡

本文报道了以峰鉴别技术为定性依据的测定火锅汤料中微量吗啡的反相高效液相色谱法。本法采用改良的Ｓｔａｓ－ｏｔｔｏ法分离提取复杂样品中的微量吗啡。由于采用峰鉴别技术，增加了定性参数，大大提高了定性方法的准确性。本法简便、快速。最低检出浓度为０．０４ｍｇ／Ｌ。在数十例样品分析中，与其它仪器分析结果比照，非常符合。     

Quality assurance system for a decomposition method as demonstrated for the Wickbold combustion technique

 A five-step model for a quality assurance system is developed for an internal quality control check. It includes the quality control of the decomposition method and the detection method as steps belonging together. The Wickbold combustion technique as decomposition method in combination with atomic absorption spectrometry was chosen. The vaporization of the elements mercury, arsenic, lead, antimony and selenium is based on combustion in an oxyhydrogen flame. To check the efficiency of the analytical system, the uncertainty of results was calculated on the basis of the "Guide to the Expression of Uncertainty in Measurement". Received: 13 January 1997 · Accepted: 29 March 1997     

科学仪器工作状态监测装置的设计与实现

建立了支持网络通讯的科学仪器工作状态监测装置。该检测装置以单片机系统为核心,运用信号检测技术获取仪器的工作状态信息,实现有效机时的记录。基于自制编码表对数据进行BASE64编码,保证了记录信息的安全;支持嵌入式互联网技术将被监测仪器的实时状态上传到远程服务器,实现大型科学仪器的网络化监测。     

Velocity map imaging of the photolysis of n-C4H9Br in UV region

Using velocity map ion imaging technique, the photodissociation of n-C₄H₉Br in the wavelength range 231–267 nm was studied. The results and our ab initio calculations indicated that the absorption of n-C₄H₉Br in the investigated region originated from the excitations to the lowest three repulsive states, as assigned as 1A″, 2A′ and 3A′ in C_s symmetry. Dissociations occurred on the PES surfaces of the three states, terminating in C₄H₉+Br (²P_3/2) or C₄H₉ + Br^* (²P_1/2) as two channels, and being impacted by an avoided crossing between the PES surfaces of the 2A′ and 3A′ states. The transition dipole to the 1A″ state was perpendicular to the symmetry plane, so perpendicular to the C–Br bond. The transitions to the 3A′ state was polarized parallel to the symmetry plane, and also parallel to the C–Br bond. While the transition dipole to the 2A′ state was in the symmetry plane, but formed an angle of about 53.1° with the C–Br bond. We have also determined the avoided crossing probabilities, which affected the relative fractions of the individual pathways, for the photolysis of n-C₄H₉Br near 234 nm and 267 nm.     

Deux methodes photothermiques a l'etude d'une preparation de contact d'un melange binaire de cristaux liquides

The photopyroelectric (PPE) method is proposed as a sensitive technique to study a binary mixture of liquid crystals in a contact preparation. The photothermal signal is generated while scanning the contact preparation. The crystal-smectic A, smectic A-nematic, and nematic-isotropic interphase boundaries are detected. The displacement of these boundaries due to the variation of the temperature is monitored. The study of these displacements allows us to draw the complete temperature-concentration phase diagram of a binary mixtures. This revised version was published online in August 2006 with corrections to the Cover Date.     

Estimation of parameters characterizing the adsorption of sodium,potassium, and rubidium cryptates at the mercury electrode/solution interface

The adsorption of complexes formed by sodium, potassium, and rubidium cations with macrobicyclic ligand (kryptofix 222 with C₁₈H₃₆N₂O₆ composition) is studied as a function of the ligand concentration on a stationary mercury drop in 0.1 M solutions of corresponding sulfates and chlorides by using the differential capacitance technique. Based on the model of two parallel capacitors supplemented by the Frumkin isotherm, the adsorption parameters of studied cryptates are estimated by using the regression analysis technique. Differential capacitance curves calculated with the parameters found are compared with experimental data. The comparison of the found adsorption parameters makes it possible to reveal the effects of the nature of included cations and specifically adsorbed supporting-electrolyte anions on the adsorption behavior of cryptates under study.__________Translated from Elektrokhimiya, Vol. 41, No. 4, 2005, pp. 475–481.Original Russian Text Copyright © 2005 by Stenina, Sviridova.     

基于核酸探针的光学传感方法和细胞成像研究

许多疾病的特征在于各种生物分子表现出的异常活性,这些物质通常在细胞内外显示过表达现象,因此对其灵敏靶向识别可以提供诊断和治疗效用。由于基因诊疗和化学传感技术的发展,用于灵敏检测细胞内外生物化学物质的核酸探针突显优势。核酸探针可以在稳定进入细胞的同时,特异性地结合目标物质,通过光学方法检测或通过成像技术标识出来。本文综述了采用光学传感方法和成像技术,基于核酸探针检测生物分子的新进展。根据检测对象进行分类,概括分析了几个代表性体系:核酸序列、蛋白质和酶、化学物质和物理化学条件,并详细阐述其关键设计原理、灵敏度及样品检测等结果,同时指出了各类核酸探针的优缺点。     

High-resolution Hadamard transform microscope fluorescence imaging: quantifying the DNA content in single cells

This study presents a novel miniaturized Hadamard transform fluorescence imaging microscope, by combining a conventional fluorescence microscope with Hadamard transform multiplexing encoding using a one-dimensional movable mask to realize spatial resolution and a linear CCD for multichannel detection. The microscope can provide high-resolution automatically-generated 0–255 gray level HT images for morphological analysis and visualization of a single cell, and normalized HT images for cellular quantitative measurements. The microscopes imaging capability was applied to measure the DNA content in human lymphocyte, chicken erythrocyte and eel erythrocyte, and a comparative study was performed. The results show that the calibrated DNA content in a chicken erythrocyte is 2.32 pg when human lymphocyte is used as the standard, and eel erythrocyte may be a potentially reliable and novel standard for determining DNA contents in other species because it has a stable DNA value of 2.06 pg, with a CV of 4.3% when 20 eel erythrocytes are measured. The results also demonstrate that the HT imaging microscope should be valuable in the fields of medicine and cell biology.     

顺磁性聚酯金属配合物的合成及其驰豫性能的研究

本文通过二乙三胺五乙酸（ＤＴＰＡ）或乙二胺四乙酸（ＥＤＴＡ）的双酸酐与二元醇或二元酚进行聚合反应，制备了两个系列共１５种新的聚酯型大分子配体及其顺磁性金属配合物，用核磁、红外光谱以及元素分析表征了配体和配合物的结构。初步试验结果表明，与相应的小分子金属配合物相比，聚酯金属配合物具有较高的弛豫性能。