Gold nanoparticle aggregation-based highly sensitive DNA detection using atomic force microscopy

The potential ability of atomic force microscopy (AFM) as a quantitative bioanalysis tool is demonstrated by using gold nanoparticles as a size enhancer in a DNA hybridization reaction. Two sets of probe DNA were functionalized on gold nanoparticles and sandwich hybridization occurred between two probe DNAs and target DNA, resulting in aggregation of the nanoparticles. At high concentrations of target DNA in the range from 100 nM to 10 μM, the aggregation of gold nanoparticles was determined by monitoring the color change with UV-vis spectroscopy. The absorption spectra broadened after the exposure of DNA–gold nanoparticles to target DNA and a new absorption band at wavelengths >600 nm was observed. However, no differences were observed in the absorption spectra of the gold nanoparticles at low concentrations of target DNA (10 pM to 10 nM) due to insufficient aggregation. AFM was used as a biosensing tool over this range of target DNA concentrations in order to monitor the aggregation of gold nanoparticles and to quantify the concentration of target DNA. Based on the AFM images, we successfully evaluated particle number and size at low concentrations of target DNA. The calibration curve obtained when mean particle aggregate diameter was plotted against concentration of target DNA showed good linearity over the range 10 pM to 10 nM, the working range for quantitative target DNA analysis. This AFM-based DNA detection technique was three orders of magnitude more sensitive than a DNA detection method based on UV-vis spectroscopy.     

最优化方法在拟合正交系晶体弹性及压电系数中的应用

由布里渊散射实验可以得到晶体弹性及压电系数。本文介绍了最优化方法在拟合正交晶体弹性及压电系数中的应用 ,并对几种主要的最优化算法的稳定性、初值选取、拟合数据结果的判断及评价等关键问题做了详细的讨论     

中性线修正型变截面梁类构件压电控制

传统绝对节点坐标法(absolute nodal coordinate formulation,ANCF)在变截面梁类构件建模过程中常以几何中位线等效构造单元中性线,难以对变截面单元位移场状态进行精确描述.为解决此类问题,本文以中细型变截面梁类构件为研究对象,深入考虑变截面结构几何因素及复合材料属性对变截面梁类构件中性...     

Electro-elastic green's functions for a piezoelectric half-space and their application

I.IntroductionItiswell'knownthatoneofthemostpowerfultoolsinlinearfieldtheoriesistheGreen'sfunction.Fore1asticity,considerableresearchcanbefoundintheliterature.However,theGreen'sfunctionforpiezoe1ectricityisratherlimitedduetotheanisotropyandelectromechanic…     

Two-dimensional electroelastic fundamental solutions for general anisotropic piezoelectric media

I.IntroductionDuetotheirintrinsiccouplingeffectbetweenmechanicalandelectricalfields,piezoelectricmaterialshavebeenwidelyusedintechnologyastransducersandsensorsand,morerecently,asactuatorsinsmartstructures.lnordertooptimizetheirmicrostructuresandunderstand…     

压电材料空间轴对称问题的通解及其应用

本文根据横观各向同性压电材料空间轴对称问题场方程的结构特点,利用逐次引进势函数的方法,最后得到将位移分量和电势函数用满足特定偏微分方程的单一势函数表示的所谓通解,推导过程表明这种形式的通解是完备的,作为应用举例,文中用通解求解了压电材料半无限体表面受集中力的问题,得到位移、应力、电位移分量及电势函数的解析表达式,本文所提供的通解可作为分析含空腔、夹杂或币形裂纹等缺陷的压电材料的机-电耦合行为的工具,算例所得结果可直接用于求解压电体相互间或压电体与普通弹性体间的接触问题。     

A general solution and the application of space axisymmetric problem in piezoelectric material

ＡＧＥＮＥＲＡＬＳＯＬＵＴＩＯＮＡＮＤＴＨＥＡＰＰＬＩＣＡＴＩＯＮＯＦＳＰＡＣＥＡＸＩＳＹＭＭＥＴＲＩＣＰＲＯＢＬＥＭＩＮＰＩＥＺＯＥＬＥＣＴＲＩＣＭＡＴＥＲＩＡＬＷａｎｇＺｉ－ｋｕｎｇ（王子昆）ＣｈｅｎＧｅｎｇ－ｃｈａｏ（陈庚超）（ＸｉａｎＪｉａｏ...     

The study of applicability for PLK method

This paper researches the applicability of the PLK method. We give the general formulae of the asymptotic solution and strained coordinates, and we establish necessary conditions for the applicability PLK method. Besides, the applicability of the approach is also exemplified by means of examples in this paper.     

电涡流传感器所受电磁力研究

本文研究电涡流传感器的探测线圈与电涡流之间的电磁作用力。理论计算与测量结果表明,对于某些应用电涡流传感器的灵敏装置而言,这个力的影响是不能忽略的。     

THE METHOD OF WEIGHTED DIFFERENCE FOR SINGULAR PERTURBATION PROBLEM

In this paper, we introduce a new difference approximation to the first order derivative u′and give a class of uniformly convergent difference schemes.