Mesoporous tungsten titanate as matrix for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis of biomolecules

In this paper, mesoporous tungsten titanate (WTiO) with different nano-pore structures was utilized as matrix for the analysis of short peptides by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS). Effect of characteristic features of mesoporous matrices on laser desorption/ionization process was investigated. Experiments showed that the ordered two-dimensional and three-dimensional mesoporous matrices were superior in performance to the non-ordered WTiO matrix. The dramatic enhancement of signal sensitivity by the ordered mesoporous matrices can be reasonably attributed to the ordered structure, which facilitated the understanding on structure-function relationship in mesoporous cavity for laser desorption process of adsorbed biomolecules. With the ordered mesoporous matrix, the short peptides are successfully detected. The presence of trace alkali metal salt effectively increased the analyte ion yields and the MALDI-TOFMS using the inorganic mesoporous matrices displayed a high salt tolerance. The developed technique also showed a satisfactory performance in peptide-mapping and amino-acid sequencing analysis.     

Changes of antioxidative enzymes and cell membrane osmosis in tomato colonized by arbuscular Mycorrhizae under NaCl stress

Salinity toxicity is a worldwide agricultural and eco-environmental problem. Many literatures show that arbuscular mycorrhizal fungi (AMF) can enhance salt tolerance of many plants and some physiological changes occurred in AM symbiosis under salt stress. However, the role of ROS-scavenging enzymes in AM tomato is still unknown in continuous salt stress. This study investigated the effect of Glomus mosseae on tomato growth, cell membrane osmosis and examined the antioxidants (superoxide-dismutase, SOD; catalase, CAT; ascorbate peroxidase, APX; peroxidase, POD) responses in roots of mycorrhizal tomato and control under different NaCl stress for 40 days in potted culture. NaCl solution (0, 0.5 and 1%) was added to organic soil in the irrigation water after 45 days inoculated by AMF (Glomus mosseae). (1) AMF inoculation improved tomato growth under salt or saltless condition and reduced cell membrane osmosis, MDA (malonaldehyde) content in salinity. So the salt tolerance of tomato was enhanced by AMF; (2) SOD, APX and POD activity in roots of AM symbiosis were significantly higher than corresponding non-AM plants in salinity or saltless condition. However, CAT activity was transiently induced by AMF and then suppressed to a level similar with non-AM seedlings; (3) higher salinity (1% level) and long stress time suppressed the effect of AMF on SOD, APX, POD and CAT activity; (4) this research suggested that the enhanced salt tolerance in AM symbiosis was mainly related with the elevated SOD, POD and APX activity by AMF which degraded more reactive oxygen species and so alleviated the cell membrane damages under salt stress. Whereas, the elevated SOD, POD and APX activity due to AMF depended on salinity environment.     

几种新型手性季铵盐-卟啉化合物的合成与表征(Ⅰ)

本文合成了四个新的Ｎ－甲基麻黄素或奎宁手性季铵盐－卟啉化合物,并用元素分析、红外光谱、紫外可见光谱、核磁共振谱、质谱确定了其结构。     

甲酸-乙酸-水-氯化钙体系汽液平衡盐效应的研究

本文采用Otsuki-Williams式汽液平衡釜测定了甲酸-乙酸-水体系及甲酸-乙酸-水-氯化钙体系在98658.28Pa下汽液平衡数据。氯化钙的加入量为0.5mol/(kg溶剂)。结合盐效应的作用情况,对相图进行了分析。研究结果表明:氯化钙的加入对体系起了物理化学作用,它与盐溶、盐析现象有关。     

甲酸-乙酸-水-醋酸钠体系汽液平衡的盐效应

采用Otsuki-Williams式汽液平衡釜测定了甲酸-乙酸-水-醋酸钠体系在9.866×10⁴Pa下汽液平衡数据。醋酸钠的加入量为2mol/(kg溶剂)。结果表明:醋酸钠对体系起物理化学作用,也起化学作用。它涉及到了两种盐对各组分的盐溶、盐析和盐效应的不规则现象。根据实验数据,绘制了汽液平衡相图、各组分挥发度等于1的曲线和甲酸-乙酸在体系中相对挥发度等于1的曲线,用以判别、分析盐效应的作用。     

火焰原子吸收光谱法测定钙盐试剂中的痕量镉

采用巯基棉分离富集钙盐试剂中的前量镉,用火焰原子吸收光谱法进行测定。方法简便、快速,结果满意。样品测定的相对标准偏差均小于４．８１％。     

盐卤硼酸盐化学研究进展

综述了近２０年来从事青藏高原新类型硼酸盐盐湖“盐卤硼酸盐化学”的研究进展。     

地质工程自动监测硬件系统若干技术问题

地质工程自动监测系统具有监测对象信息变化差异大,监测环境恶劣等显着特点。其硬件系统须具备监测范围广,抗干扰能力强,抗雷击等特殊功能。同时,为了适应现代化工程建设的要求,还应具备先进的智能化和网络化等功能。为此,本文对地质工程自动监测系统的功能和组成、当前的发展趋势及所涉及到一些关键硬件技术问题展开了讨论,着重总结了作者有关地质工程自动监测硬件系统关键技术问题的研究。最后介绍了作者为五强溪水电站边坡工程建立的自动监测系统,以及该系统双端防雷保护和模块化信号调理等难题的解决。     

清江隔河岩坝基工程岩体质量评价研究

采用《工程岩体分级标准》定性和定量相结合的方法,根据岩石坚硬程度和岩石完整程度决定岩体的基本质量,然后考虑地下水状态、初始应力状态、工程轴线方位与主要软弱结构面产状的组合关系等修正因素,确定坝基工程岩体的级别。针对隔河岩工程考虑岩溶、层间剪切带、应力水平、岩体所处工程部位等因素,给出隔河岩工程坝基工程岩体分级。     

锚杆锚固质量无损检测的研究

本文介绍了一种无损检测锚杆锚固质量的新技术。通过模型试验,分析了声频应力波在锚固体系中的反射相位特征和能量衰减变化规律,探讨了测定锚固力的无损拉拔试验,并将结果成功应用于潘一矿巷道锚杆锚固工程的施工质量检测。