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991.
992.
研究了姜黄素–邻二氮菲–镍配合物修饰电极对葡萄糖的催化氧化行为。在pH 6.0的磷酸盐缓冲液条件下,姜黄素与邻二氮菲–镍配合物聚合,附着在铂碳电极上制得姜黄素–邻二氮菲–镍配合物修饰电极,该修饰电极对葡萄糖的催化效果优于姜黄素修饰电极。在优化测试条件下,姜黄素–邻二氮菲–镍配合物修饰电极对葡萄糖测定的线性范围为6.0×10–5~3.0×10–3 mol/L,相关系数r=0.997 2。该修饰电极可用于测定血液中的葡萄糖,测定结果的相对标准偏差为2.16%~4.44%(n=10),回收率在98.2%~103.2%之间。该方法准确可靠,可用于测定血液中的葡萄糖。 相似文献
993.
通过静态实验探讨了表面活性剂十二烷基硫酸钠(SDS)改性沸石吸附碱性品红的影响因素。实验结果表明在投入量为8 g/L、吸附时间为45 min、p H值为6、温度为25℃时,对初始质量浓度100 mg/L碱性品红溶液的去除率可达到96.16%,吸附量为12.02 mg/g;SDS改性沸石吸附碱性品红的吸附性能符合Langmuir型吸附等温线和一级反应动力学模型的特征;热力学分析结果表明,吸附符合自发吸热过程。 相似文献
994.
995.
温敏材料由于优异的性能和潜在的应用价值而具有良好的发展前景.利用超分子自组装单层(SAM)与表面引发聚合(SIP)技术将2-(2-甲氧乙氧基)甲基丙烯酸乙酯(MEO2MA)与聚乙二醇甲基丙烯酸酯(OEGMA526)的共聚物poly(MEO2MAco-OEGMA526)接枝于金表面,探索了不同引发剂溶液浓度(χIsol)、单体OEGMA526摩尔浓度(C526)与干态膜厚度(d)对该高分子刷性质的影响.应用石英晶体微天平(QCM)对其温敏行为进行研究,结果表明:在χIsol=1%与C526=5%条件下制备的高分子刷,最低临界溶解温度(LCST)为34℃;其LCST由OEGMA526的单体摩尔浓度决定,不受膜厚的影响.该高分子刷在接枝生物素后其与链霉亲和素的结合实验证明,高分子刷末端的羟基为其官能团化提供了契机.该易衍生化温敏高分子刷为发展新型温敏材料提供了研究基础. 相似文献
996.
Oksana V. Gnedenko Yury V. MezentsevAndrey A. Molnar Andrey V. LisitsaAlexis S. Ivanov Alexander I. Archakov 《Analytica chimica acta》2013
A highly sensitive reverse sandwich immunoassay for the detection of human cardiac myoglobin (cMb) in serum was designed utilizing a gold nanoparticle (AuNP)-enhanced surface plasmon resonance (SPR) biosensor. First, a monoclonal anti-cMb antibody (Mab1) was covalently immobilized on the sensor surface. AuNPs were covalently conjugated to the second monoclonal anti-cMb antibody (Mab2) to form an immuno-gold reagent (Mab2-AuNP). The reverse sandwich immunoassay consists of two steps: (1) mixing the serum sample with Mab2-AuNP and incubation for the formation of cMb/Mab2-AuNP complexes and (2) sample injection over the sensor surface and evaluation of the Mab1/cMb/Mab2-AuNP complex formation, with the subsequent calculation of the cMb concentration in the serum. The biosensor signal was amplified approximately 30-fold compared with the direct reaction of cMb with Mab1 on the sensor surface. The limit of detection of cMb in a human blood serum sample was found to be as low as 10 pM (approx. 0.18 ng mL−1), and the inter-assay coefficient of variation was less than 3%. Thus, the developed SPR-based reverse sandwich immunoassay has a sensitivity that is sufficient to measure cMb across a wide range of normal and pathological concentrations, allowing an adequate estimation of the disease severity and the monitoring of treatment. 相似文献
997.
Nanoparticles with desirable properties not exhibited by the bulk material can be readily synthesized because of rapid technological developments in the fields of materials science and nanotechnology. In particular their highly attractive electrochemical properties and electrocatalytic activity have facilitated achievement of the high level of signal amplification needed for the development of ultrasensitive electrochemical affinity biosensors for the detection of proteins and DNA. This review article explains the basic principles of nanoparticle based electrochemical biosensors, highlights the recent advances in the development of nanoparticle based signal amplification strategies, and provides a critical assessment of the likely drawbacks associated with each strategy. Finally, future perspectives for achieving advanced signal simplification in nanoparticles based biosensors are considered. 相似文献
998.
A method of interference fringe processing by finding the average and the extremum gray levels of image points within a fixed or varying window and then computing the modified image intensity with a simple algorithm is proposed. This method can yield a uniform brightness and high (1 or near to 1) contrast at the same time over the whole image area for an input pattern with low contrast and considerable luminance variation, when a relatively large window is used. It can also perform extremum extraction if a small window is employed. This technique is simple, effective and convenient for use, as has been verified by a series of examples of simulated and experimental fringe processing. 相似文献
999.
We have developed a highly sensitive analytical technique for detecting the distribution of surfactants existing on a polymer surface. We studied the chemical modification of surfactants with the hydroxyl group by using amine-containing compounds in the gaseous phase at 23 °C; then, we performed measurements by using time-of-flight secondary ion mass spectrometry (TOF-SIMS). We found that 4-(dimethylamino) phenyl isocyanate as a modification reagent is capable of modifying the hydroxyl group when 1,8-diazabicyclo[5,4,0]undec-7-ene (DBU) is used as a catalytic agent. We demonstrated this modification with stearyl alcohol on a polymer material surface. The signal sensitivity of stearyl alcohol after the modification increased considerably in comparison with that of the unmodified stearyl alcohol. Moreover, we confirmed that this modification method can be used for the distribution analysis of surfactants. The distribution conditions of traces of surfactants with the hydroxyl group can be observed clearly by using this modification method. 相似文献
1000.
Md. Mosarraf Hossain Gyanendra Sheoran Dalip Singh Mehta Chandra Shakher 《Optics and Lasers in Engineering》2007,45(6):684-689
A simple method for contouring of diffused objects by using lensless Fourier transform digital holography (LFTDH) and dual-index immersion method is presented. Depth contour interval up to minimum of 0.12 mm could be achieved without making the interference phase fringes over crowded. 相似文献