Empirical predictor of conditions that support ideal-filter capillary electrophoresis

Ideal-filter CE (IFCE) is a method for the selection of affinity binders for protein targets from oligonucleotide libraries, for example, random-sequence oligonucleotide libraries and DNA-encoded libraries, in a single step of partitioning. In IFCE, protein–oligonucleotide complexes and unbound oligonucleotides move in the opposite directions, facilitating very high efficiency of their partitioning. For any given protein target and oligonucleotide library, protein–oligonucleotide complexes and unbound oligonucleotides move in the opposite directions only for a limited range of EOF mobilities, which, in turn, corresponds to a limited range of pH and ionic strength values of the running buffer. Rational design of IFCE-based partitioning requires a priori knowledge of this range of pH and ionic strength values, and here we introduce an approach to predict this range for a given type of the running buffer. The approach involves measuring EOF mobilities for a relatively wide range of pH and ionic strength (I) values and finding an empirical predictor function that related the EOF mobility with pH and ionic strength. In this work, we developed a predictor function for a running buffer (Tris-HCl) that is commonly used in CE-based partitioning of affinity binders for protein targets. This predictor function can be immediately used for the rational design of IFCE-based partitioning in this running buffer, while the described approach will be used to develop predictor functions for other types of running buffer if needed.     

Analytic Invariant Charge in QCD with Suppression of Nonperturbative Contributions at Large <Emphasis Type="Italic">Q</Emphasis><Superscript>2</Superscript>

Based on the analytic invariant charge obtained from the results of the standard perturbation theory up to the four-loop approximation, we construct a “synthetic” model of the invariant charge in quantum chromodynamics. In the suggested model, the perturbative discontinuity on the timelike semiaxis in the complex Q² plane is preserved, and nonperturbative contributions not only cancel nonphysical perturbation theory singularities in the infrared region but also rapidly decrease in the ultraviolet region. On one hand, the effective coupling function in this model is enhanced at zero (the dual superconductivity property of the quantum chromodynamics vacuum); on the other hand, a dynamical gluon mass appears. In our approach, fixing the parameter corresponding to the string tension parameter and normalizing (for example, at the point M_τ) entirely fix the synthetic invariant-charge model. The dynamical gluon mass m_g is then fixed and is stable as the number of loops of the original perturbative approximation increases. __________ Translated from Teoreticheskaya i Matematicheskaya Fizika, Vol. 145, No. 2, pp. 221–239, November, 2005.     

长寿命斯特林制冷机膨胀机无摩擦运行技术

论述了长寿命斯特林制冷机膨胀机的结构,重点对排出器活塞与冷指气缸无接触运行控制方法进行论述.制冷机正在进行加速寿命跑和,运行很好.     

评定磨合表面的最佳形貌参数

磨合是新机器设备投入使用之前的必经过程，而且对摩件的表面形貌都要发生明显的变化，因此，如何评定磨合表面形貌值得深入研究，实际上，磨合是对摩件的表面形貌由初始状态逐渐趋于相互吻合的过程，因而磨合之后配对表面的形貌具有相关性，在此基础上上，研究提出了利用互相关结构函数评定磨合，这种评定方法具有能够同时反映两个表面形貌和对表面形貌变化反映敏感，以及能够将表面轮廓的峰和谷分开考虑等优点，理论分析和应用实例     

毛细管电泳迁移时间重现性影响因素的探讨

以电泳介质为研究对象,在毛细管区带电泳(CZE)和胶束电动毛细管色谱(MECC或MEKC)两种电泳模式下,探讨了电泳 介质中各组分浓度、冲洗程序、电泳介质在实验中发生的变化对溶质迁移时间重现性的影响。根据描述缓冲液中各组分 浓度与电渗流迁移时间和溶质迁移时间的关系式,证明利用电导值可准确地表征电泳缓冲液的浓度;通过控制缓冲液的电 导值可提高迁移时间的重现性。运行缓冲液pH值的变化影响毛细管壁上的硅羟基的电离,因此需选择合理的冲洗程序使硅 羟基的电离达到平衡,以提高迁移时间的重现性。毛细管入口端缓冲液是影响溶质迁移行为的主要因素,其在电泳中发生 的变化是影响电泳重现性的重要原因。目前在实验中可通过提高运行缓冲液的更换频率来保证迁移时间的重现性。     

Error analysis of corner cutting algorithms

Corner cutting algorithms are used in different fields and, in particular, play a relevant role in Computer Aided Geometric Design. Evaluation algorithms such as the de Casteljau algorithm for polynomials and the de Boor–Cox algorithm for B‐splines are examples of corner cutting algorithms. Here backward and forward error analysis of corner cutting algorithms are performed. The running error is also analyzed and as a consequence the general algorithm is modified to include the computation of an error bound. This revised version was published online in June 2006 with corrections to the Cover Date.     

磨合过程对流体润滑性能影响的数值分析数学模型

建立了非牛顿流体热弹流润滑数学模型,对重载条件下磨合过程进行数值分析,在考虑具有粘塑性本构的非牛顿流体时,采用了高效粘度法对建立的方程进行了解算。结果表明,热解的摩擦系数明显低于等温解的摩擦系数,当进一步考虑流体的非牛顿效应时,不仅温升的结果更为符合实际,摩擦系数也更接近试验结果。     

服役导线的力学性能退化研究

为更清楚地了解阳江35kV服役导线的运行状态,从分析导线断线原因出发,通过对老旧导线的单丝断裂强度、弹性模量、应力应变曲线、拉断力、抗拉强度等综合指标的测试与分析,研究服役导线的力学性能,由试验结果得出:35kV老旧导线钢芯的直径变化对于架空导线的外径变化有直接的影响,老旧导线的铝线直径变化较小,其外径增大1.67%~4.24%;钢单线的拉断应力仍能达到标准中规定值的106%,且伸长率也满足要求;部分铝线的抗拉强度低于95%;绞线拉断力仍能达到95%的计算拉断力。但是相同规格下的老旧导线的抗拉强度比新导线的抗拉强度低2%~18%。结果表明:阳江地区服役三十年以上的老旧导线在力学性能上仍能继续承载。     

Update of g-2 of the muon and Δα

We update our Standard Model predictions for g-2 of the muon and for the hadronic contributions （5） 2 to the running of the QED coupling, Δαbad^5）（MZ^2）. Particular emphasis is put on recent changes in the hadronic contributions from new data in the 2π channel and from the energy region just below 2 GeV.