Quadrature demodulation technique for self-mixing interferometry displacement sensor

A new, to the best of our knowledge, signal processing method based on quadrature demodulation technique is presented for laser diode self-mixing interferometry(LDSMI) displacement sensor. Phase modulation of the laser beam is obtained by an electro-optic modulator (EOM) in the external cavity. Detection of the target's displacement can be easily achieved by sampling the interference signal at those times which satisfied certain conditions. The major advantage of the technique is that it does not involve any complicated calculation and insensitive to the sampling error. Experimental results show that the proposed method can effectively improve the displacement measurement resolution of the laser diode self-mixing displacement sensor to a few nanometers.     

基于低通滤波的相位掩模干涉图相位解调方法

 传统的四步相移算法在分析相位掩模干涉图时存在相移值误差、分辨力降低和相干噪声等不足,从而影响了动态干涉仪的性能。为了提高相位掩模干涉图的分析精度,提出了一种基于低通滤波的相位解调方法。该方法根据相位掩模引入相位的空间频率远大于被测相位空间频率的最大值,采用低通滤波的方法提取相位信息。数值分析结果表明,该方法的精度高于传统四步相移算法。分析干涉图的信噪比,合理选择低通滤波器,可进一步提高相位解调的精度。     

Noise-induced synchronous stochastic oscillations small scale cultured heart-cell networks

This paper reports that the synchronous integer multiple oscillations of heart-cell networks or clusters are observed in the biology experiment. The behaviour of the integer multiple rhythm is a transition between super- and sub- threshold oscillations, the stochastic mechanism of the transition is identified. The similar synchronized oscillations are theoretically reproduced in the stochastic network composed of heterogeneous cells whose behaviours are chosen as excitable or oscillatory states near a Hopf bifurcation point. The parameter regions of coupling strength and noise density that the complex oscillatory rhythms can be simulated are identified. The results show that the rhythm results from a simple stochastic alternating process between super- and sub-threshold oscillations. Studies on single heart cells forming these clusters reveal excitable or oscillatory state nearby a Hopf bifurcation point underpinning the stochastic alternation. In discussion, the results are related to some abnormal heartbeat rhythms such as the sinus arrest.     

一类耦合非线性振子同步混沌Hopf分岔及其电路仿真

对于一类同时存在扩散耦合和梯度耦合的非线性振子系统, 通过空间傅里叶变换，得到具有不同波矢的各运动模式的相互独立的运动方程. 计算各横截模的Lyapunov指数, 可在耦合参数平面上确定同步混沌的稳定区域. 在稳定区域边界, 一对共轭横截模式失稳，导致同步混沌的Hopf分岔. 对耦合Lorenz振子系统进行了数值模拟，并设计了耦合Lorenz振子系统的电路, 进行耦合振子系统同步混沌Hopf分岔的电路仿真实验. 计算和仿真的结果表明，Hopf分岔的特征频率等于失稳横截模式的振荡频率. 关键词： 耦合非线性振子 同步混沌 横截模式 电路仿真     

导数-同步荧光光谱法直接测定尿样中的洛美沙星

研究了洛美沙星在不同 pH值介质中的荧光特性 ,发现在 pH =3 3时 ,洛美沙星荧光波长有 4 0nm红移 ,据此建立了一种导数 同步荧光光谱法直接测定尿样中洛美沙星的新方法。经样品测定 ,其检测限为0 35mg·L- 1 ,平均回收率为 97%～ 10 4 % ,相对标准偏差为 0 83%～ 1 6 2 %。     

咔唑、蒽和苝的同时恒能量同步荧光法分析

建立了咔唑、蒽和苝的同时恒能量同步荧光分析法。对方法的优越性进行了比较说明。实验是在自制带恒能量扫描多功能荧光分光计上进行的。咔唑、蒽和苝的检测限分别为1ng/ml、0.8ng/ml和0.5ng/ml,相对标准偏差不大于4.4％。     

Signal enhancement of a novel multi-address coding lidar backscatters based on a combined technique of demodulation and wavelet de-noising

Multi-address coding (MAC) lidar is a novel lidar system recently developed by our laboratory. By applying a new combined technique of multi-address encoding, multiplexing and decoding, range resolution is effectively improved. In data processing, a signal enhancement method involving laser signal demodulation and wavelet de-noising in the downlink is proposed to improve the signal to noise ratio (SNR) of raw signal and the capability of remote application. In this paper, the working mechanism of MAC lidar is introduced and the implementation of encoding and decoding is also illustrated. We focus on the signal enhancement method and provide the mathematical model and analysis of an algorithm on the basis of the combined method of demodulation and wavelet de-noising. The experimental results and analysis demonstrate that the signal enhancement approach improves the SNR of raw data. Overall, compared with conventional lidar system, MAC lidar achieves a higher resolution and better de-noising performance in long-range detection.     

盐酸吡格列酮与BSA反应机理的荧光猝灭法、同步荧光法比较研究

利用荧光猝灭法和同步荧光法进行了盐酸吡格列酮(PGH)与牛血清白蛋白(BSA)之间反应机理的比较研究.结果表明:两种方法在研究PGH-BSA体系的猝灭类型、结合常数、结合位置、作用力类型、协同性及能量转移距离等方面,得到了相同的结果,表明同步荧光光谱法可以用于药物与蛋白反应机理的研究.     

基于跳频通信的移动机械遥控系统

为解决在同一个工作区域多个无线通信网络容易彼此干扰无法协同工作的问题,以移动机械工程车辆为控制对象,设计JRCJ500无线遥控系统,由用户手持终端接收用户操作指令,现场控制器驱动远程设备动作,实现对车辆的远程操作控制与状态信息反馈;采用STM32L低功耗处理器设计无线通信模块,选择公用通信频段,基于跳频扩频通信技术,约定无线通信数据格式与跳频频点,通过简单高效的跳频图案生成算法,在每一帧数据中添加同步字头,实现了无线通信网络的快速同步组网,保证了通信的稳定性,实现了在限定工作区域多台无线通信设备同时工作的应用需求。由于采用模块化设计,系统具有良好的通用性和可移植性,能够在多种复杂恶劣工作场合实现远程控制功能。     

A fluorescence anisotropy method for measuring protein concentration in complex cell culture media

The rapid, quantitative analysis of the complex cell culture media used in biopharmaceutical manufacturing is of critical importance. Requirements for cell culture media composition profiling, or changes in specific analyte concentrations (e.g. amino acids in the media or product protein in the bioprocess broth) often necessitate the use of complicated analytical methods and extensive sample handling. Rapid spectroscopic methods like multi-dimensional fluorescence (MDF) spectroscopy have been successfully applied for the routine determination of compositional changes in cell culture media and bioprocess broths. Quantifying macromolecules in cell culture media is a specific challenge as there is a need to implement measurements rapidly on the prepared media. However, the use of standard fluorescence spectroscopy is complicated by the emission overlap from many media components. Here, we demonstrate how combining anisotropy measurements with standard total synchronous fluorescence spectroscopy (TSFS) provides a rapid, accurate quantitation method for cell culture media. Anisotropy provides emission resolution between large and small fluorophores while TSFS provides a robust measurement space. Model cell culture media was prepared using yeastolate (2.5 mg mL^–1) spiked with bovine serum albumin (0 to 5 mg mL^–1). Using this method, protein emission is clearly discriminated from background yeastolate emission, allowing for accurate bovine serum albumin (BSA) quantification over a 0.1 to 4.0 mg mL^–1range with a limit of detection (LOD) of 13.8 μg mL^–1.