| 导数-同步荧光光谱法直接测定尿样中的洛美沙星 |
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| 引用本文： | 许庆琴,杜黎明,王静萍.导数-同步荧光光谱法直接测定尿样中的洛美沙星[J].光谱学与光谱分析,2002,22(3):444-445. |
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| 作者姓名： | 许庆琴 杜黎明 王静萍 |
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| 作者单位： | 山西师范大学分析测试中心,山西,临汾,041004 |
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| 基金项目： | 山西省自然科学基金 (No 2 0 0 1 1 0 1 6)资助课题 |
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| 摘 要： | 研究了洛美沙星在不同 pH值介质中的荧光特性 ,发现在 pH =3 3时 ,洛美沙星荧光波长有 4 0nm红移 ,据此建立了一种导数 同步荧光光谱法直接测定尿样中洛美沙星的新方法。经样品测定 ,其检测限为0 35mg·L- 1 ,平均回收率为 97%～ 10 4 % ,相对标准偏差为 0 83%～ 1 6 2 %。
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| 关 键 词： | 直接测定 洛美沙星 导数－同步荧光光谱法 尿样 抗菌素 血药浓度 |
| 文章编号： | 1000-0593(2002)03-0444-02 |
| 修稿时间： | 2000年12月30 |
Study on the Direct Determination of Lomefloxacin in Urine by Derivative-synchronous Fluorescence |
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| XU Qing-qin,DU Li-ming,WANG Jing ping Center of Analysis and Test,Shanxi Normal University,Linfen ,China.Study on the Direct Determination of Lomefloxacin in Urine by Derivative-synchronous Fluorescence[J].Spectroscopy and Spectral Analysis,2002,22(3):444-445. |
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| Authors: | XU Qing-qin DU Li-ming WANG Jing ping Center of Analysis and Test Shanxi Normal University Linfen China |
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| Institution: | Center of Analysis and Test, Shanxi Normal University, Linfen 041004, China. |
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| Abstract: | The LMX fluorescence properties at various pH of media were studied. The experiments indicated that LMX fluorescence emission wavelength is red shifted 40 nm at pH value of 3.3. The background interference was eliminated effectively. Under this condition, a new method for the determination of lomefloxacin in human urine by derivative-synchronous fluorescence has been developed. The linear equation of LMX can be obtained as dF/d lambda = -6.642c-24.143, of which the correlation coefficient is 0.9996. When concentrations of LMX were within the range of 0.35-28.10 mg.L-1, there exists a good linear relationship between the concentration and fluorescence intensity of LMX. The detection limit was 0.35 mg.L-1. The average recoveries of LMX were 97%-104% and the RSD were 0.83%-1.62%. |
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| Keywords: | Lomefloxacin Derivative synchronous fluorescences Urine |
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