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21.
徐凯宇 《应用数学和力学(英文版)》1995,16(10):951-959
ANALYSISOFSTABILITYONELASTICPLATESWITHINITIALIMPERFECTIONSXuKaiyu(徐凯宇)(ReceivedOct.5.1994;CommunicatedPaiLizhou)ANALYSISOFSTA... 相似文献
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以内源荧光光谱和荧光相图法研究了脲和盐酸胍诱导的卵清溶菌酶分子的去折叠过程,结果表明,当变性液中脲和盐酸胍的浓度分别约为4.0和3.0 mol/L时,卵清溶菌酶分子的去折叠过程均存在一个折叠中间态,这两个去折叠过程均符合"三态模型".在卵清溶菌酶分子"三态"去折叠过程的基础上,通过变性剂分子和卵清溶菌酶分子之间的缔合一... 相似文献
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Yan YAN Rui Xian LIU Yin Mao WEI Ye Hua SHEN Xin Du GENG Institute of Modern Separation Science Northwest University Xi’an Contemporary Educational Technology Center Northwest University Xi’an 《中国化学快报》2006,17(1):105-108
Hydrophobic interaction chromatography (HIC) is widely used for the separation and purification of biopolymers in their native state, and now also becomes a new method to refold the denatured proteins1. The effect of salt or water concentration2 on the pr… 相似文献
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Nile red bound to human serum albumin (HSA) shows an order of magnitude increase in the probe's fluorescence intensity. Here, we report on the fluorescence characteristics of the probe-protein complex in Trizma buffer (pH 7.1), urea, guanidine hydrochloride, and AOT/isooctane/buffer reverse micelles using both steady—state and time-resolved fluorescence techniques. With a view to illustrating the use of extrinsic probe fluorescence spectroscopy in protein research, we demonstrate that protein unfolding can be observed through measurements of the probe's time-resolved anisotropy and steady-state fluorescence spectrum. Moreover, this shows that thermal unfolding is fundamentally different from using denaturant, with respect to changes in both the nanosecond diffusional rotation of the probe at intermediate stages and in the denatured protein's structure. Also, the large Stokes shift of Nile red allows the changes in the environment of the probe-protein complex in reverse micelles of varying waterpool size to be easily identified in the steady-state fluorescence. This was not seen in earlier work exploiting the intrinsic tryptophan fluorescence of HSA and further demonstrates the complementary information that extrinsic fluorescence probe studies can offer protein science. We discuss the complex acrylamide quenching characteristics of Nile red bound to HSA in terms of the possibility of at least two binding sites for the probe and the effect of acrylamide on the probe-protein structure at very high quencher concentrations. 相似文献
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Abstract The formation of protein aggregates after pressure treatment was investigated by Fourier Transform Infrared Spectroscopy (FTIR). The results show that the pressure unfolded protein ends in a conformation, after the release of the pressure, which has an increased tendency to aggregate, even at temperatures lower than the denaturation temperature of the untreated protein. After pressure pretreatment the infrared spectrum shows the same intermolecular antiparallel β-structure features as observed after a temperature treatment that gives rise to protein gelation. on the other hand, this structure can be destabilized by applying moderate pressures, which are significantly lower than the corresponding denaturation pressure. 相似文献
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Small angle neutron scattering (SANS) has been used to study conformational changes in protein bovine serum albumin (BSA)
as induced by varying temperature and in the presence of protein denaturating agents urea and surfactant. BSA has prolate
ellipsoidal shape and is found to be stable up to 60°C above which it denaturates and subsequently leads to aggregation. The
protein solution exhibits a fractal structure at temperatures above 64°C, with fractal dimension increasing with temperature.
BSA protein is found to unfold in the presence of urea at concentrations greater than 4 M and acquires a random coil Gaussian
chain conformation. The conformation of the unfolded protein in the presence of surfactant has been determined directly using
contrast variation SANS measurements by contrast matching surfactant molecules. The protein acquires a random coil Gaussian
conformation on unfolding with its radius of gyration increasing with increase in surfactant concentration
相似文献
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A dendrimeric trimer undergoes folding and unfolding in response to a chemical stimulus. The trimer of interest contains a central dendrimer with a butadiyne‐linked zinc porphyrin dimer ((ZnP)2) core, in addition to two terminal dendrimers with zinc porphyrin (ZnP) cores. The obtained absorption spectra indicate that the unfolded form is the exclusive conformer in chloroform, while the addition of 1,4‐diazabicyclo[2.2.2]octane (DABCO) in chloroform leads to transformation from the unfolded to the folded structure containing two DABCO units per trimer; the folded structure originates from the cross‐linking of (ZnP)2 and ZnP with DABCO. Moreover, the addition of excess DABCO promotes the generation of the unfolded structure containing four DABCO units. 相似文献