| 脲和盐酸胍诱导的卵清溶菌酶分子去折叠过程中各稳定构象态的分布和过渡 |
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| 引用本文： | 张潭,边六交.脲和盐酸胍诱导的卵清溶菌酶分子去折叠过程中各稳定构象态的分布和过渡[J].高等学校化学学报,2011,32(7):1497-1505. |
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| 作者姓名： | 张潭 边六交 |
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| 作者单位： | 西北大学生命科学学院,西安,710069 |
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| 基金项目： | 国家自然科学基金(批准号:21075097)资助 |
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| 摘 要： | 以内源荧光光谱和荧光相图法研究了脲和盐酸胍诱导的卵清溶菌酶分子的去折叠过程,结果表明,当变性液中脲和盐酸胍的浓度分别约为4.0和3.0 mol/L时,卵清溶菌酶分子的去折叠过程均存在一个折叠中间态,这两个去折叠过程均符合"三态模型".在卵清溶菌酶分子"三态"去折叠过程的基础上,通过变性剂分子和卵清溶菌酶分子之间的缔合一...
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| 关 键 词： | 卵清溶菌酶 去折叠 脲 盐酸胍 特征展开参数 |
| 收稿时间： | 2010-09-13 |
Distribution and Transition of Stable Conformations of Hen Egg White Lysozymes in Their Unfolding Induced by Urea and Guanidine Hydrochloride Solutions |
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| ZHANG Tan,BIAN Liu-Tao.Distribution and Transition of Stable Conformations of Hen Egg White Lysozymes in Their Unfolding Induced by Urea and Guanidine Hydrochloride Solutions[J].Chemical Research In Chinese Universities,2011,32(7):1497-1505. |
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| Authors: | ZHANG Tan BIAN Liu-Tao |
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| Institution: | ZHANG Tan,BIAN Liu-Jiao(College of Life Science,Northwest University,Xi'an 710069,China) |
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| Abstract: | The unfolding of hen egg white lysozymes induced by urea and guanidine hydrochloride was studied by intrinsic fluorescence emission spectra and fluorescence phase diagram.The results show that during these two unfolding procedures,a stable intermediate of hen egg white lysozymes separately existed at about 4.0 mol/L of urea or 3.0 mol/L of guanidine hydrochloride in denaturation solution,and both of these two unfolding followed a three-state model.Based on the three-state model,an equation which described t... |
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| Keywords: | Hen egg white lysozyme Unfolding Urea Guanidine hydrochloride Characteristic unfolding parameter |
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