非放射性碘标记-电感耦合等离子体质谱用于免疫分析研究

研究了非放射性碘标记-电感耦合等离子体质谱免疫分析体系,该体系以兔抗大肠杆菌作为模型抗原,羊抗兔IgG蛋白为抗体,建立了一种新的免疫分析方法。实验中采用溴代琥珀酰胺(NBS)为氧化剂,实现非放射性127I标记羊抗兔IgG蛋白,探索了标记的最佳条件,标记率为63.12％; 标记物在Sephadex G50柱上分离纯化后,研究了I-羊抗兔蛋白的稳定性,结果表明,标记物在4 ℃放置96 h后几乎没有碘脱落,并保持一定的活性。实验中采用聚苯乙烯96孔板作为固相载体进行免疫反应,以电感耦合等离子体质谱为检测手段,方法的检出限为0.12 mg·L-1,RSD(n=9)为3%; 该体系也可适用于其他活性蛋白、核酸等的标记和分析。     

孝感市1996年碘缺乏病防治监测结果分析

1996年9月采用PPS法在孝感市范围内对碘缺乏病(IDD)防治工作作了抽样监测。8～10岁儿童甲状腺肿大率(TGR)842%,其中查出Ⅱ°肿大3人,TGR≤5%的点占5333%;尿碘值<100μg/L占417%;M值为3855μg/L;居户盐碘2388×10-6±1216×10-6,<20×10-6的点占1667%,非碘盐占1167%。监测结果表明,本市8～10岁儿童TGR较1994年有下降;碘营养缺乏状况得到改善,碘代谢水平已达到IDD消除标准;碘盐的合格率仅7658%,离IDD消除标准还差近14个百分点。在本市IDD防治工作中实施的以供应加碘盐为主的综合防治措施是行之有效的,并取得了一定效果。揭示在今后的防治中,要进一步抓紧以供应加碘盐为主的综合防治措施。     

微波辐射下分子碘催化合成双吲哚甲烷衍生物

在无溶剂微波辐射下,芳醛或亚胺在中性三氧化二铝吸附的单质碘催化下快速地与吲哚发生亲电取代反应制得双吲哚甲烷衍生物(收率70％一97％),其结构经^1H NMR,IR和元素分析表征。     

无溶剂条件下碘催化不对称双吲哚的合成

在室温以及无溶剂条件下,吲哚与烷基-3-吲哚基甲醇在碘的催化下以73％～99％的高收率得到不对称双吲哚烷基化合物。其结构经^1H NMR,IR及MS表征。     

Convenient Synthesis of Benziodazolone: New Reagents for Direct Esterification of Alcohols and Amidation of Amines

Hypervalent iodine heterocycles represent one of the important classes of hypervalent iodine reagents with many applications in organic synthesis. This paper reports a simple and convenient synthesis of benziodazolones by the reaction of readily available iodobenzamides with m-chloroperoxybenzoic acid in acetonitrile at room temperature. The structure of one of these new iodine heterocycles was confirmed by X-ray analysis. In combination with PPh₃ and pyridine, these benziodazolones can smoothly react with alcohols or amines to produce the corresponding esters or amides of 3-chlorobenzoic acid, respectively. It was found that the novel benziodazolone reagent reacts more efficiently than the analogous benziodoxolone reagent in this esterification.     

Studies on the Interaction of Poly(I:C) with Heterocyclic Compounds Containing Iodine

The iodine heterocvclic compound with trivalent-iodine as the cation has a specially remarkable antitumor activityii^[1]. Although 3, 6-bis (dimethylamino)-dibenzopyriodonium formate([I⁺]) inhibits DNA biosynthesis of cancer cells in vitro, the way of [I⁺] depressing DNA synthesis has not been studied. It is significant to investigate the mechanism of the interaction of [I⁺] with nucleic acids.     

混合表面活性剂在非极性溶剂中的聚集行为

表面活性剂在非极性溶剂中的聚集行为比在水溶液中复杂得多. 水溶液中表面活性剂有一明确的临界胶束浓度(CMC),而在非极性溶剂中至今对CM C概念仍有怀疑^[1], 但已有多种手段如染料增溶法、水增溶法、光散射法、荧光偏振、紫外和核磁共振谱等证实并测定了非极性溶剂中 CMC 的存在^[1～5]. 表面活性剂在非极性溶剂中以非离子化状态存在, 其缔合主要靠两亲分子之间的偶极-偶极以及离子对相互作用, 那么在一种表面活性剂溶液中加入另一种表面活性剂, 即表面活性剂的复配, 必然对其聚集行为产生重大影响, 但迄今为止, 尚未见关于混合表面活性剂在非极性溶剂中聚集行为的报道. 本文采用碘光谱法和水增溶法测定了阴离子表面活性剂AOT 和非离子表面活性剂 Brij30 混合后在正庚烷中形成反胶束的 CMC, 以期考察表面活性剂的复配对其聚集行为的影响。     

Visible absorption of iodine-doped polysilane with p-N,N-dialkylaminophenyl substituents

The doping effects on optical and electrical properties of polysilanes were investigated by in-situ measurement. When polysilanes bearing a p-N,N-dialkylaminophenyl substituent were doped with iodine vapor, drastic spectral changes were observed. The evolved visible absorption up to 700 nm was found to be strong and stable compared to that of iodine-doped polysilanes such as poly(methylphenylsilane). On the basis of the studies concerning iodine doping of the related p-N,N-dimethylaminophenyl-substituted silane and disilane, and also theoretical calculations, this new absorption can be best interpreted by the result of charge transfer consisting of the strong interaction between iodine and dialkylamino substituents in the polysilanes. © 1996 John Wiley & Sons, Inc.     

Cobaloximes as Environmentally Advantageous Alternatives to Organotin Hydrides in Iodine Atom Abstraction Routes to Benzyl Radicals

High yields of alkyl radicals derived from alkylcobaloximes have been achieved using tungsten light (or in some cases ultrasound) radiation in both organic and aqueous media. These improved yields are obtained when pyridine (the usual base ligand) is replaced by suitably bulky lone-pair donors or water. The alkyl radicals so generated take part in iodine abstraction reactions with benzyl iodide giving benzyl radicals which may be trapped in near-quantitative yield with 2,2,6,6-tetramethylpiperidinyloxy (TEMPO) or in good yield with lepidinium (4-methylquinolinium) trifluoroethanoate or lepidinium camphor-10-sulphonate. The usual drawbacks of cost and tedious work-up procedures associated with the more commonly used organotin hydride reagents are avoided.     

碘掺杂聚苯胺催化合成环己酮1,2-丙二醇缩酮

合成了聚苯胺-碘(PAn-I2)催化剂,并对其结构进行了表征。并通过环己酮与1,2-丙二醇合成环己酮1,2-丙二醇缩酮,探讨了PAn-I2对缩酮反应的催化活性。实验结果表明,PAn-I2是缩酮反应的良好催化剂,在环己酮100 mmol,n(环己酮)∶n(1,2-丙二醇)=1∶1.5,催化剂用量为反应物总质量的3%,环己烷12 mL,反应时间2 h的优化条件下,环己酮1,2-丙二醇缩酮的收率可达92.1%。