全文获取类型
收费全文 | 217篇 |
免费 | 12篇 |
国内免费 | 56篇 |
专业分类
化学 | 224篇 |
综合类 | 12篇 |
物理学 | 49篇 |
出版年
2023年 | 2篇 |
2022年 | 1篇 |
2021年 | 7篇 |
2020年 | 1篇 |
2019年 | 3篇 |
2018年 | 7篇 |
2017年 | 10篇 |
2016年 | 6篇 |
2015年 | 7篇 |
2014年 | 5篇 |
2013年 | 16篇 |
2012年 | 21篇 |
2011年 | 12篇 |
2010年 | 20篇 |
2009年 | 13篇 |
2008年 | 21篇 |
2007年 | 21篇 |
2006年 | 10篇 |
2005年 | 13篇 |
2004年 | 14篇 |
2003年 | 8篇 |
2002年 | 10篇 |
2001年 | 3篇 |
2000年 | 9篇 |
1999年 | 5篇 |
1998年 | 7篇 |
1997年 | 4篇 |
1996年 | 5篇 |
1995年 | 6篇 |
1994年 | 2篇 |
1993年 | 2篇 |
1992年 | 3篇 |
1991年 | 1篇 |
1990年 | 1篇 |
1989年 | 1篇 |
1988年 | 1篇 |
1987年 | 1篇 |
1984年 | 4篇 |
1978年 | 1篇 |
1976年 | 1篇 |
排序方式: 共有285条查询结果,搜索用时 15 毫秒
81.
Yusuke Inaba 《Tetrahedron》2004,60(13):3097-3107
Mono(imidazolyl)-substituted Co(II) porphyrin dimer with a ‘picket fence’ structure was synthesized as a new artificial hemoglobin model containing two binding sites. The dimer was confirmed by UV-vis, resonance Raman and ESR spectral measurements to bind two dioxygen molecules reversibly. The dioxygen binding affinity of the dimer was lower than that of the corresponding monomer. The decrease in this affinity is discussed in terms of steric hindrance and orientational effect of the axial ligand. 相似文献
82.
83.
根据基体电极的不同,对血红蛋白电化学分析检测进行了综述,包括:血红蛋白的银电极检测和玻碳修饰电极检测等方法的应用和各方法对血红蛋白检测的研究现状。 相似文献
84.
Jeong-Yeol Yoon Jung Hun Lee Jung-Hyun Kim Woo-Sik Kim 《Colloids and surfaces. B, Biointerfaces》1998,10(6):628-377
Submicron microspheres were used directly without ligand coupling for the batch and continuous separations of proteins. In the batch experiments for separating BSA (bovine serum albumin) from BHb (bovine hemoglobin), introducing both hydrophobic effects for BSA and electrostatic repulsion for BHb (and vice versa) was required for high selectivity, and microspheres with low number density of surface groups were advantageous. For the continuous experiments, the utilization of a stirred cell was successful, where the microspheres were in the form of latex with good dispersion of particles. The flow rate without a pump was 0.5–1.3 ml min−1, and the ratio of BSA and BHb was varied. In the experiments for eliminating BHb from BSA, elution curves of BHb corresponded to the single component breakthrough curves, while those for BSA did not. The latter is believed to be due to the interference by BHb in the adsorption of BSA. 相似文献
85.
纳米ZnO修饰电极的制备及其对血红蛋白, 细胞色素c的直接电化学研究 总被引:2,自引:0,他引:2
A novel electrochemical method as a sensitive and convenient technique for the determination of heme proteins based on their interaction with ZnO nanorods was developed. A ZnO nanorod modified glassy carbon electrode (ZnO/GCE) was prepared and the electrochemical behaviors of heme proteins, such as hemoglobin (HB) and cytochrome c (Cyt-c), on this modified electrode have been studied. The results showed that both HB and Cyt-c could be oxidized on the modified electrode and the oxidation currents were linear to the concentrations of the analytes in aqueous solutions. In addition, the results of flow injection analysis (FIA) further suggested the high stability and reproducibility of the ZnO nanorod modified electrode. So this method can be applied to the determination of HB and Cyt-c in biological systems. 相似文献
86.
The redox reaction of poly(ethylene oxide) (PEO)-modified hemoglobin (PEO–Hb) was analyzed in PEO oligomers with cyclic voltammetry. The PEO–Hb was made soluble in PEO with molecular weight of 200 (PEO200) containing 0.5 M KCI. Quasi-reversible redox signals of PEO–Hb were obtained by using an indium tin oxide (ITO) glass working electrode. PEO–Hb, cast on the ITO electrode, also showed the redox response in PEO with molecular weight of 400 (PEO400). The peak current of PEO–Hb on the ITO electrode gradually increased during potential cycling. The effect of the scan rate on the quantity of electricity (Q) was analyzed after the peak current reached a constant value. The constant Q value was observed at the scan rate ranging from 30 to 500 mV/sec. The number of reactive PEO–Hb molecules was estimated from this constant Q-value. It was suggested that the electron transfer was carried out at the first layer of the PEO–Hb which was in direct contact with the ITO electrode. The quantity of electricity of PEO–Hb increased when the ITO electrode was first washed in an aqueous medium with ultrasonicator. This strongly suggested that the more effective surface area of the ITO electrode turned to be covered with PEO–Hb when the microporous region of the ITO particles was more hydrated. 相似文献
87.
StudyofPreparationandCharacteristicsofPyridoxalated PolyhemoglobinLiangShuguang;TongMingrong;PanJilunandYuYaoting(Institutefo... 相似文献
88.
89.
90.
We have studied the fluorescent properties of flavonols (quercetin, fisetin, morin, rutin) with the aim of studying possible
interaction with hemoglobin and bovine serum albumin (BSA). We observed an increase in the intensity of intrinsic fluorescence
for all the flavonols except rutin in the presence of BSA. From the changes in the fluorescence spectra, we concluded that
tautomeric forms are formed on interaction with hemoglobin. We determined the interconnection between the structure of related
flavonols and their fluorescent properties on interaction with proteins, and we determined the binding constants for binding
with BSA and hemoglobin.
__________
Translated from Zhurnal Prikladnoi Spektroskopii, Vol. 74, No. 5, pp. 659–664, September–October, 2007. 相似文献