纳米CoHCF修饰电极的制备及其对血红蛋白的电化学测定研究

A cobalt hexacyanoferrate （CoHCF） nanoparticle （size ca. 60 nm） chemically modified electrode （CME） was fabricated and the electrochemical behavior of hemoglobin （Hb） at this nanosized CoHCF CME was studied. In comparison with a bare glassy carbon electrode （GCE） and a general CoHCF CME electrodeposited in a traditional manner, the present nanosized CoHCF CME performed efficiently electrocatalytic reduction for Hb with relatively high sensitivity, stability, and longlife, Combined with liquid chromatography （LC）, the nanosized CoHCF CME was used as the electrochemical detector of Hb in the established flow injection analysis-electrochemical determination （FIA-ECD） system. The peak current was a linear function of concentrations in the range from 2.5×10^-8 to 5.0×10^-6mol/L for Hb, with detection limit of 1.4×10^-8 mol/L. The FIA-ECD system has been successfully applied to assess the Hb content of clinic blood samples with advantages of sensitiveness, speediness, easy control and small sample-consumption.     

纳米TiO2膜用于光催化氧化测定化学需氧量的研究

A photocatalytic oxidation method for determination of chemical oxygen demand （COD） using nano-TiO2 film, based on the use of a nano-TiO2-Ce（SO4）2 system and electrochemical detection, was proposed. The technique was originated from the direct determination of the Ce（Ⅲ） concentration change resulting from photocatalytic oxidation of organic compounds. Ce（Ⅲ）, which was produced by photocatalytic reduction of Ce（SO4）2, could be measured at a multi-walled carbon nanotubes （MWNT） chemically modified electrode （CME）. The COD values by this method were calculated from the differential pulse voltammetry （DPV） current of Ce（Ⅲ） at the CME. Under the optimal operation conditions, the detection limit of 0.5 mg·L^-1 COD with the linear range of 1-600 mg·L^-1 was achieved. This method was also applied to determination of various COD of ground water and wastewater samples. The resuits were in good agreement with those from the conventional COD methods, i.e., permanganate and dichromate ones.     

PVP/Pd/IrO_2/Nafion修饰微电极用于成纤维细胞中一氧化氮释放的研究

采用PVP/Pd/IrO_2/Nafion修饰电极对成纤维细胞中NO的释放情况进行了研究 。结果表明,在正常状态下,采用NO前体L-精氨酸和乙酰胆碱对成纤维细胞进行刺 激后没有NO的释放;当用脂多糖进行诱导后,则释放出高浓度的NO,加入L-精氨酸 和乙酰胆碱都促进了NO的合成,而L-NNA的加入则逆转了L-精氨酸和乙酰胆碱的作 用。     

过氧化聚吡咯膜修饰电极色谱电化学用于帕金森试验动物的研究

研制了一种新型的过氧化聚吡咯膜修饰电极（OPPy/CME)作为液相色谱电化学检测器,该电极可以用来检测生物体内的单胺类神经递质及其代谢产物。用药物建立了帕金森动物试验模型,对不同情况下的试验动物脑内单胺类神经递质及其代谢产物通过微渗析取样和液相色谱电化学检测联用技术进行了测定。初步探讨了试验动物产生帕金森病的机理,为研制和筛选更有效的治疗帕金森病的新药提供了一种准确、可靠的分析手段。     

纳米ZnO修饰电极的制备及其对血红蛋白, 细胞色素c的直接电化学研究

A novel electrochemical method as a sensitive and convenient technique for the determination of heme proteins based on their interaction with ZnO nanorods was developed. A ZnO nanorod modified glassy carbon electrode (ZnO/GCE) was prepared and the electrochemical behaviors of heme proteins, such as hemoglobin (HB) and cytochrome c (Cyt-c), on this modified electrode have been studied. The results showed that both HB and Cyt-c could be oxidized on the modified electrode and the oxidation currents were linear to the concentrations of the analytes in aqueous solutions. In addition, the results of flow injection analysis (FIA) further suggested the high stability and reproducibility of the ZnO nanorod modified electrode. So this method can be applied to the determination of HB and Cyt-c in biological systems.     

分层次电化学实验教学改革

在教学实践的基础上,提出了分层次、多模块的电化学实验教学模式,并从教学改革理念、教学内容设计以及教学方法和培养模式等方面进行了阐述。     

电聚合制备分子印迹电极及其特异性表征

本实验设计以邻苯二胺为功能单体,以双酚A为目标分子,采用简单快速的电聚合方法,制备了对双酚A具有特异性识别能力的分子印迹电极。采用差分脉冲伏安法和石英晶体微天平对印迹电极的特异识别性能进行表征。结果表明,印迹电极对双酚A表现出较高的特异性识别能力。与非印迹电极相比,识别能力提高一个数量级。同时,该电极对100倍浓度的阿特拉津、17β-雌二醇的信号响应仅为十分之一,表明该电极在复杂体系中具有良好的抗干扰能力。此外,该实验方案目标分子选择灵活,能制备针对不同目标分子的印迹电极,可满足自主设计实验的需求。通过该创新实验,可以有效帮助学生深入了解分子间的相互作用,体验在分子层次设计材料的过程。     

Direct and Simultaneous Determination of Phenol, Hydroquinone and Nitrophenol at Boron-Doped Diamond Film Electrode

The electrochemical characteristics of multi-component phenolic pollutants, such as phenol （Ph）, hydroquinone （HQ） and 4-nitrophenol （4-NP）, were investigated on boron-doped diamond （BDD） film electrode by differential pulse voltammetry （DPV） technique. A simple and feasible platform was accordingly established for the direct and simultaneous determination of these three phenolic pollutants. Results showed that, Ph, HQ and 4-NP gave obvious oxidation peaks on BDD electrode at the potential of 1.24, 0.76 and 1.52 V, respectively. Each of them displayed good linear relationship between their oxidation peak currents and their corresponding concentrations in a rather wide range coexisting with one or two of the other phenolic pollutants. The detection limits of Ph, HQ and 4-NP were estimated to be as low as 1.82×10^-6, 1.67×10^-6 and 1.44×10^-6 mol·L^-1, respectively. Therefore, a promising direct and simultaneous electrochemical determination method of multi-component phenolic pollutants in wastewater samples was constructed successfully on BDD electrode with advantages being rapid, simple, convenient, sensitive, in situ and inexpensive.     

掺硼金刚石膜电极表面产生羟基自由基的原位ESR研究

建立了原位电化学-电子顺磁共振(ESR)方法,系统研究了掺硼金刚石(BDD)膜电极表面在水溶液中的羟基自由基(·OH)产生规律.结果表明,在高于析氧电位时,·OH的生成速率随着阳极电位的升高、电流密度的增加而增大,增加速率逐渐减缓.与此同时,与氢终端的BDD膜电极相比,氧终端的BDD膜电极表面因具有较好的亲水性而具有更高的·OH产生能力.在电化学氧化处理有机废水的过程中,电极表面处于氧终端的状态,有利于·OH产生并保持较高的活性.溶液pH值也在一定程度上影响·OH生成反应,酸性溶液中,BDD膜电极表面的·OH产生能力强于在中性或碱性溶液中.进一步研究发现,BDD膜电极表面还可能生成O3-·0自由基.本研究为深入探索BDD膜电极表面·OH的产生机制提供了研究手段,为阐述BDD膜电极电极在污染物处理中高效性的本质提供了有效的证据.     

中性红掺杂SiO2纳米粒子修饰的酶阵列传感器同时测定葡萄糖、乳酸和L-谷氨酸的研究

本文以中性红为核，二氧化硅为壳，利用反相微乳液技术，通过正硅酸四乙酯的水解制备了掺杂有中性红的二氧化硅纳米粒子，并用TEM技术进行了表征。核中性红能够催化测定葡萄糖，乳酸和L-谷氨酸的反应，而壳二氧化硅不仅克服了电活性物质中性红易流失的缺点，且具有高的生物亲和性。分别与葡萄糖氧化酶、乳酸氧化酶以及L-谷氨酸氧化酶混合后，修饰在碳阵列电极表面。最后在该酶阵列电极表面滴加一层Nafion, 防止电活性物质抗坏血酸、尿酸等的干扰。该酶阵列传感器与流动注射分析技术(FIA)相结合，可应用于同时检测大鼠血样中的葡萄糖，乳酸和L-谷氨酸浓度。该方法无需通过传统的色谱柱的分离，大大简化了实验条件，为这一领域的研究提供了有效的分析方法。